Abstract
This study aimed to investigate the use of leptin as a marker for gestational diabetes by analyzing any correlation between serum leptin levels versus oral glucose tolerance tests (at 24 to 28 weeks of pregnancy) and increased body weight (during pregnancy). A total of 110 female cases (81 pregnant and 29 non-pregnant) were included in the study. The 81 pregnant cases were divided into 3 groups according to their oral glucose tolerance test results. A chi-square test was used for categorical variables. The distribution of numerical variables was tested with the Shapiro–Wilk test. ANOVA and a post-hoc Bonferroni test was used for parametric data. Kruskal–Wallis variance analysis was used for non-parametric data. The Mann–Whitney U-test was used for pairwise comparisons. Spearman correlation analysis and multivariate regression analysis were performed for the evaluation of the correlation analysis between the parameters. Oral glucose tolerance test results were compared with leptin levels with a cut-off value of 11.43 for leptin. The ROC curve demonstrated an 83.3% sensitivity and 72.1% specificity for leptin. Leptin may play a role in the pathophysiology of gestational diabetes mellitus. However, the relationship between leptin levels and maternal weight gain during pregnancy is still unknown.
Similar content being viewed by others
References
Engelgau MM, Herman WH, Smith PJ, German RR, Aubert RE. The epidemiology of diabetes and pregnancy in the US., 1988. Diabetes Care. 1995;18(7):1029–33.
Yalçın HR, Zorlu CG. Threshold value of glucoses creening tests in pregnancy: could it be standardized for every population? Am J Perinatol. 1996;13(5):317–20.
Tanır HM, Sener T, Gürer H, Kaya M. A ten-year gestational diabetes mellitus cohort at a university clinic of mid-Anatolian region of Turkey. Clin Exp Obstet Gynecol. 2005;32(4):241–4.
Öncül M, Uludağ S, Şen C, et al. The role of maternal serum leptin and malondialdehyde levels in screening and diagnosis of gestational diabetes mellitus. Perinatol Journal. 2009;17(1). http://www.perinataljournal.com/20090171003
Uludağ S, Gezer A. Gebelik diyabeti. TJOD-Uzmanlık sonrası eğitim ve güncel gelişmeler. 2005;2:55–61.
American Diabetes Association. Standards of medical care in diabetes. Diabetes Care. 2010;33(Suppl 1):11–61.
Metzger BE, Buchanan TA, Coustan DR, et al. Summary and recommendations of the fifth international workshop-conference on gestational diabetes mellitus. Diabetes Care. 2007;30(Suppl 2):S251–60.
Fantuzzi G, Faggioni R. Leptin in the regulation of immunity, inflammation, and hematopoiesis. J Leukoc Biol. 2000;68(4):437–46.
American Diabetes Association. Standards of medical care in diabetes-2012. Diabetes Care. 2012;35(Suppl 1):11–63.
Metzger BE, Coustan DR. Summary and recommendations of the Fourth International Workshop-conference on Gestational Diabetes Mellitus. The Organizing Committee. Diabetes Care. 1998;21(Suppl 2):161–7.
O’Sullivan JB, Mahan CM. Criteria for the oral glucose tolerance test in pregnancy. Diabetes. 1964;13:278–85.
Sokup A, Ruszkowska-Ciastek B, Góralczyk K, et al. Insulin resistance as estimated by the homeostatic method at diagnosis of gestational diabetes: estimation of disease severity and therapeutic needs in a population-based study. BMC Endocr Disord. 2013;13:21.
Señarís R, Garcia-Caballero T, Casabiell X, et al. Synthesis of leptin in human placenta. Endocrinology. 1997;138(10):4501–4.
Saad MF, Khan A, Sharma A, et al. Physiological insulinemia acutely modulates plasma leptin. Diabetes. 1998;47(4):544–9.
Schubring C, Englaro P, Siebler T, et al. Longitudinal analysis of maternal serum leptin levels during pregnancy, at birth and up to six weeks after birth: relation to body mass index, skin-folds, sex steroids and umbilical cord blood leptin levels. Hormone Res. 1998;50(5):276–83.
Kautzky-Willer A, Pacini G, Tura A, et al. Increased plasma leptin in gestational diabetes. Diabetologia. 2001;44(2):164–72.
Qiu C, Willams MA, Vadachkoria S, Frederick IO, Luthy DA. Increased maternal plasma leptin in early pregnancy and risk of gestational diabetes mellitus. Obstet Gynecol. 2004;103(3):519–25.
Maghbooli Z, Hossein-Nezhad A, Rahmani M, Shafaei AR, Larijani B. Relationship between leptin concentration and insulin resistance. Horm Metab Res. 2007;39(12):903–7.
Soheilykhah S, Mojibian M, Rahimi-Saghand S, Rashidi M, Hadinedoushan H. Maternal serum leptin concentration in gestational diabetes. Taiwan J Obstet Gynecol. 2011;50(2):149–53.
Goel R, Chugh K, Duhan N, et al. Maternal serum leptin concentration in gestational diabetes mellitus. IJIR 2016;2(2):414–22. https://www.researchgate.net/publication/310353790_Maternal_serum_leptin_concentration_in_gestational_diabetes_mellitus. Accessed 27 Jun 2019.
Şengül ÖB, Mungan T, Erdemoğlu E, İslamoğlu G, Kıyak N. Investigation of the correlation between 100 gram oral glucose tolerance test results and maternal leptin levels during pregnancy. J Turk Ger Gynecol Assoc. 2009;10(3):158–61.
Mantzoros CS, Moschos SJ. Leptin: in search of role(s) in human physiology and pathophysiology. Clin Endocrinol (Oxf). 1998;49(5):551–67.
McGregor GP, Desega JF, Ehlenz K, et al. Radiommunological measurement of leptin in plasma of obese and diabetic human subjects. Endocrinology. 1996;137(4):1501–4.
Genova M, Todorova K, Tzatchev K. Level of leptin in pregnant women with risk for diabetes. 18th Meeting of Balkan Clinical Laboratory Federation, 22–25 September, 2010,Tirana, Albania. http://nt-cmb.musofia.bg:8080/jspui/bitstream/10861/173/1/Level%20of%20leptin%20in%20pregnant%20women%20with%20risk%20for%20diabetes.pdf.
Chen ZQ, Cheng Y, Su HY, et al. Clinical study of maternal serum leptin levels as a predictor of gestational diabetes mellitus and gestational impaired glucose tolerance. Zhonghua Fu Chan Ke ZaZhi. 2005;40(3):164–7.
Mao F, Niu XB, Gu S, Ji L, Wei BJ, Wang HB. Investigation of leptin G19A polymorphism with bladder cancer risk: a case-control study. J Clin Lab Anal. 2020;34(8):e23351.
Özlem Ş, Mungan T, Erdemoğlu E, İslamoğlu G, Cantürk F. Cord blood leptin levels in gestational diabetes. Gynecol Obstet Reprod Med. 2010;16(2):70–4.
Mertoglu C, Gunay M, Gungor M, Kulhan M, Kulhan NG. A study of inflammatory markers in gestational diabetes mellitus. Gynecol Obstet Reprod Med. 2018;25(1):1.
Arslan E, Gorkem U, Togrul C. Is there an association between kisspeptin levels and gestational diabetes mellitus? Gynecol Obstet Reprod Med. 2019;26(3):179–83.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Cengiz, T., Öncel, M., Toka Özer, T. et al. Association of Leptin with Glucose Intolerance and Gestational Diabetes Mellitus in Pregnant Women: Prospective Analytical Case–Control Study. Reprod. Sci. 31, 773–778 (2024). https://doi.org/10.1007/s43032-023-01354-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s43032-023-01354-1