Abstract
Cancer is one of the world’s major causes of death. The aim of this study is to examine the acute effects of resveratrol on testicular toxicity, oxidative stress, and apoptosis caused by MTX, which is widely used in the treatment of many diseases, especially cancer, histochemically, immunohistochemically, and biochemical methods using different parameters. A total of 32 Wistar albino male rats were randomly divided into 4 groups: control, resveratrol (RES), MTX, and MTX + RES, with 8 animals in each group. At the end of the experiment, tissue and blood samples were taken, and histochemical, immunohistochemical, and biochemical parameters were examined. In this study, where parameters were compared for the first time, total thiol (TT) and native thiol (NT) are the highest in the RES group, disulfide (DS), and ischemia-modified albumin (IMA) are the highest in the MTX group. Total oxidant status (TOS) and oxidative stress index (OSI) are the highest in the MTX group, and total antioxidant status (TAS) is the highest in the RES group. Separation and deterioration in the tunica albuginea, congestion and edema in the interstitial region, vacuolization in the seminiferous epithelium, and spermatogenic serial cells spilling into the lumen without completing their maturation were observed. When examined in terms of histochemical, immunohistochemical, and biochemical examinations, our study revealed that resveratrol has positive effects on methotrexate-induced acute testicular damage, oxidative stress, and apoptosis.
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The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Research reported in this publication was supported by a grant from this work was supported by the Research Fund of Suleyman Demirel University. Suleyman Demirel University Faculty of Medicine (Ethics Committee No. 13–2/2019), and it was supported by the SDU Scientific Research Projects Unit with the project number TDK-2020–8034. The content is solely the responsibility of the authors and does not necessarily represent the official views of Suleyman Demirel University.
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Sarman, E., Gulle, K. & Ilhan, I. Histochemical, Immunohistochemical, and Biochemical Investigation of the Effect of Resveratrol on Testicular Damage Caused by Methotrexate (MTX). Reprod. Sci. 30, 3315–3324 (2023). https://doi.org/10.1007/s43032-023-01269-x
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DOI: https://doi.org/10.1007/s43032-023-01269-x