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Effects of Aerobic Exercise Combined with Oyster Peptide Supplement on the Formation of CTX-induced Late-Onset Hypogonadism in Male Rats

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Abstract

The purpose of this study is to investigate the effect of aerobic exercise (AE) training and/or oyster peptide (OP) supplementation on the formation of late-onset hypogonadism (LOH). AE training and/or OP supplement was performed during Cytoxan (CTX)-induced LOH formation in male SD rats for 6 consecutive weeks. Low dose of CTX could decrease mating times, the levels of luteinizing hormone (LH), total testosterone (TT), free testosterone (FT) in serum and TT, androgen receptor (AR), androgen binding protein (ABP), and glutathione peroxidase (GSH-Px) in testicle, but increase capture latency, mating latency, and malondialdehyde, and downregulate the mRNA expression of steroidogenic acute regulatory (StAR), P450 cholesterol side chain cleavage enzyme (P450scc), and StAR-related lipid transfer domain 7 (StARD7) in testicle. Every change was altered by AE training combined with OP supplement significantly, except for serum LH. Moreover, the effect of AE training combined with OP supplement was better than that of AE training on serum TT, FSH, testicular TT, mating latency, capture times, and mating times. AE training combined with OP supplement during CTX-induced LOH formation can prevent the LOH development by enhancing pituitary-gonads axis’s function and reducing testicular oxidative stress to promote testosterone synthesis and spermatogenesis.

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Funding

This work was supported by National Key Research and Development project in China, Grant/Award Number: 2016YFD0400603, and Open Project of the Beijing Engineering Research Center of Protein and Functional Peptides, Grant/Award Number: 2017PFP002.

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  1. Wenting Shi, Yu Liu, and Qiguan Jin have contributed equally to this work.

Authors and Affiliations

  1. College of Physical Education, Yangzhou University, Yangzhou, 225127, China

    Wenting Shi, Yu Liu, Qiguan Jin, Meitong Wu, Qizheng Sun & Zheng Li

  2. Engineering Laboratory for Agro Biomass Recycling & Valorizing, College of Engineering, China Agricultural University, Beijing, 100083, China

    Wenying Liu

  3. China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing, 100015, China

    Wenying Liu

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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Wenting Shi, Yu Liu, Qiguan Jin, Meitong Wu, Qizheng Sun, Zheng Li, and Wenying Liu. The first draft of the manuscript was written by Wenting Shi and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Qiguan Jin or Wenying Liu.

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The study was conducted according to the guidelines of the Declaration of Helsinki. The experimental procedures were approved by the Experimental Animal Management Committee and Experimental Animal Ethics Committee of Yangzhou University (YzuDWLL-201804–001).

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Shi, W., Liu, Y., Jin, Q. et al. Effects of Aerobic Exercise Combined with Oyster Peptide Supplement on the Formation of CTX-induced Late-Onset Hypogonadism in Male Rats. Reprod. Sci. 30, 1291–1305 (2023). https://doi.org/10.1007/s43032-022-01068-w

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