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Dynamic Autophagy Map in Mouse Female Germ Cells Throughout the Fetal to Postnatal Life

  • Reproductive Biology: Original Article
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Abstract

Autophagy plays vital roles in mouse female germ cells, but the potential mechanism is largely unknown. In this study, by interrogating single-cell RNA-seq dataset, we investigated the dynamic expression of autophagy-related genes in seven types of germ cells (mitosis, pre-leptotene, leptotene, zygotene, pachytene, diplotene, and dictyate) and discovered stage-specific autophagy-related genes. Using immunofluorescence (IF) and transmission electron microscopy (TEM), autophagy activity and autophagosome numbers were revealed from mitosis to follicular assembly (E12.5 (embryonic day 12.5) to P5 (postnatal day 5)). Furthermore, single-sample gene set enrichment analysis (ssGSEA) was performed to validate the autophagy kinetics from E12.5 to P5. Our study proved that the mitosis, diplotene, and dictyate female germ cells had relatively higher autophagy activity among the seven subtypes. In summary, our work provided an autophagy map, suggesting that autophagy was complicated in mouse female germ cell development from the fetal to postnatal life, which paved a new insight for deciphering the autophagy regulatory networks for cell-fate transition and female infertility issues like primary ovarian insufficiency (POI).

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Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 81971415), and the National Natural Science Foundation of China (No. 32070833).

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  1. Gurong Jiang, Li Zhang, and Jiexiang Zhao contributed equally to this study.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China

    Gurong Jiang, Zhenqin Huang & Zhijian Wang

  2. Department of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, Southern Medical University, Guangzhou, 510515, China

    Li Zhang & Lin Li

  3. Dongguan People’s Hospital, Southern Medical University, Dongguan, 523059, China

    Jiexiang Zhao

  4. Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China

    Jiexiang Zhao

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  1. Gurong Jiang
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Contributions

GR Jiang and JX Zhao performed experiments. GR Jiang and L Zhang performed bioinformatics analysis. GR Jiang, L Zhang, JX Zhao, L Li, and ZQ Huang wrote the manuscript. ZJ Wang conceived the study.

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Correspondence to Zhijian Wang.

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Jiang, G., Zhang, L., Zhao, J. et al. Dynamic Autophagy Map in Mouse Female Germ Cells Throughout the Fetal to Postnatal Life. Reprod. Sci. 30, 169–180 (2023). https://doi.org/10.1007/s43032-022-00940-z

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