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Diet Supplemented with Chrysophyllum albidum G. Don (Sapotaceae) Fruit Pulp Improves Reproductive Function in Hypertensive Male Rats

  • Male Reproduction: Original Article
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Abstract

Hypertension has been implicated as a risk factor of reproductive disorders. High blood pressure may trigger impaired sperm quality and biomarkers of reproductive disorders. This study aims to investigate the effect of diet supplemented with Chrysophyllum albidum fruit pulp (FP) on sperm parameters, reproductive hormones, and antioxidant markers in testes and epididymis of hypertensive rats. Male Wistar rats were divided into seven groups (n = 10): normotensive control rats [NC], cyclosporine (25 mg/kg)-induced hypertensive rats [Hypert], hypertensive rats treated with captopril (10 mg/kg/day) [Hypert + Capt], hypertensive [Hypert + 2%FP and Hypert + 4%FP], and normotensive [2%FP and 4%FP] rats treated with 2% and 4% of diet supplemented with African star apple fruit’s pulp [FP]. Hemodynamic parameters (arterial pressure, diastolic, and systolic pressure), sperm count, sperm motility, reproductive hormones, reactive oxygen species, and malondialdehyde levels were assessed. Diet supplemented with FP fed to hypertensive rats reduced mean arterial pressure, diastolic and systolic blood pressure, and heart rate. Furthermore, FP improved sperm quality in hypertensive rats by increasing sperm count, sperm motility with a concomitant reduction in sperm abnormality. FP also increased 3β and 17β-hydroxysteroid hydrogenase (3β-HSD and 17β –HSD) activities, as well as testosterone, luteinizing hormone, and follicle-stimulating hormone levels. Besides, FP triggered a significant increase in 3β-HSD, 17β –HSD, and STAR expression in rats’ testicular tissues. Diet supplemented with FP also reduced ROS and malondialdehyde levels and triggered an increase in thiol levels, catalase, and glutathione-S-transferase activities. This study revealed that FP supplemented diet improved sexual function in cyclosporine-induced hypertensive rats by reducing blood pressure and modulation of sperm parameters, steroidogenic enzymes, and reproductive hormones.

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Acknowledgements

The authors appreciate Dr. Nnaemeka T. Asogwa’s technical assistance at the Central Research and Diagnostic Laboratory, 132, University Road, Tanke, Ilorin, Kwara State, Nigeria. The authors did not received funding, grant, or any other support from any organization.

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Authors and Affiliations

  1. Department of Biochemistry, Ekiti State University, Private Mail Bag 5363, Ado-Ekiti, Nigeria

    Seun F. Akomolafe

  2. Department of Biochemistry and Microbiology, University of Fort Hare, Alice Eastern Cape, South Africa

    Tosin A. Olasehinde

  3. Nutrition and Toxicology Division, Food Technology Department, Federal Institute of Industrial Research Oshodi, Lagos, Nigeria

    Tosin A. Olasehinde

  4. Department of Basic Medical Science, College of Health Science and Technology, Ijero Ekiti, Nigeria

    Iyabo F. Oladapo

  5. Biomedical Technology Department, Federal University of Technology, Akure, Nigeria

    Sunday I. Oyeleye

  6. Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, Nigeria

    Sunday I. Oyeleye

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  1. Seun F. Akomolafe
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Correspondence to Seun F. Akomolafe.

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This study was performed with the Guide for the Care and Use of Laboratory Animals prepared by the Science National Academy and the Health National Institute. Approval was granted by the Ethics Committee of Ekiti State University ORD/AD/EAC/19/0072).

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The institutional ethics committee approved the study ORD/AD/EAC/19/0072). The authors also followed the Guide for the Care and Use of Laboratory Animals prepared by the Science National Academy and the Health National Institute.

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Akomolafe, S.F., Olasehinde, T.A., Oladapo, I.F. et al. Diet Supplemented with Chrysophyllum albidum G. Don (Sapotaceae) Fruit Pulp Improves Reproductive Function in Hypertensive Male Rats. Reprod. Sci. 29, 540–556 (2022). https://doi.org/10.1007/s43032-021-00746-5

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