Abstract
Recurrent implantation failure (RIF) is one of the major obstacles in IVF. Transcriptomic literature has revealed the various biological processes involved in endometrial receptivity (ER) under different physiological circumstances, especially in natural cycle. We intended to determine the function-specific ER profile under controlled ovarian stimulation (COS) cycle. This can help to back trace the genomic impairment in RIF patients during the IVF cycle and to validate the genes involved in enriched pathways. In our study, retrospective gene expression microarray dataset was reanalysed after the follow-up, in classic non pregnant RIF (cases) vs fertile women (controls) under COS (n = 5/group). Reanalysis of microarray revealed significant downregulation of cell adhesion function (P:3.11E-05) with the maximum gene count. For validation purpose, downregulation of eight genes (COMP, HABP2, ITGAD, CDH3, COL22A1, MFAP4, THBS1and CD300A) involved in enriched cell adhesion pathway having fold change > 3 were assessed by real-time PCR in independent cohorts of cases and controls (n = 24, each). Downregulation of six out of eight genes (COMP, HABP2, ITGAD, CDH3, MFAP4 and THBS1) were confirmed by real-time PCR (P < 0.05) with fold change > 2. This indicates the importance of analysed genes in the ER mechanism under COS, thus mimicking the fresh embryo transfer. The further analysis in larger cohorts would substantiate the study findings in RIF patients undergoing IVF cycle.
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Acknowledgements
We are grateful to National Health and Education Society of P. D Hinduja Hospital and Medical Research Centre for funding the study (Grant No: 1050-16-IH). We are thankful to Late Dr Kusum Zaveri for her considerable contribution in the study.
Funding
This study was funded by “National Health and Education Society” of P.D. Hinduja Hospital and Medical Research centre (Grant number 1050–16-IH).
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This study was performed in line with the principles of the Declaration of Helsinki. The study was approved by the Institutional Review Board of P.D. Hinduja Hospital and Medical research centre (Date: 27th December 2016, No. 1050–16-IH[MRC]).
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Pathare, A.D.S., Hinduja, I. Endometrial Expression of Cell Adhesion Genes in Recurrent Implantation Failure Patients in Ongoing IVF Cycle. Reprod. Sci. 29, 513–523 (2022). https://doi.org/10.1007/s43032-021-00708-x
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DOI: https://doi.org/10.1007/s43032-021-00708-x