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lncRNA HLA Complex Group 18 (HCG18) Facilitated Cell Proliferation, Invasion, and Migration of Prostate Cancer Through Modulating miR-370-3p/DDX3X Axis

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Abstract

Long non-coding RNAs (lncRNAs) have been reported to exert critical functions in the malignant development of many cancers. lncRNA HLA complex group 18 (HCG18) has been confirmed to have a promoting effect on various cancers. However, whether HCG18 functions in PC is still unclear. Therefore, the current study aimed at unveiling the role of HCG18 in PC progression and its regulatory mechanism on the biological behaviors of PC. Here, RT-qPCR was utilized to detect HCG18 expression, and then, functional experiments were conducted to verify the effects of HCG18 on PC cell proliferation, migration, invasion, and apoptosis. According to the results, HCG18 was significantly up-regulated in PC cells and it facilitated cell proliferation, migration, and invasion in PC. Furthermore, a series of mechanism experiments were carried out to verify the relationship among HCG18, miR-370-3p, and DEAD-box helicase 3 X-linked(DDX3X) in PC cells. Final rescue assays showed that DDX3X overexpression could reverse the inhibitory function of silencing HCG18 on PC progression. In summary, our study showed that lncRNA HCG18 accelerated cell proliferation, invasion, and migration of PC via up-regulating DDX3X through sponging miR-370-3p, providing a novel finding about PC-related regulatory mechanism.

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Authors and Affiliations

  1. Department of Urology, Yinzhou Hospital Affiliated to Medical School of Ningbo University, Ningbo, 315040, Zhejiang, China

    Xiaobo Pan, Guangyao Chen & Wenhao Hu

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  1. Xiaobo Pan
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  2. Guangyao Chen
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  3. Wenhao Hu
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Correspondence to Xiaobo Pan.

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Figure S1.

(A-B) Colony formation and EdU experiments assessed cell proliferative capability in different transfection groups. (C-D) Transwell assay was applied for detecting cell migration and invasion in different transfection groups. (E) Cell apoptosis was estimated through TUNEL assays in different transfection groups. (PNG 2663 kb)

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Pan, X., Chen, G. & Hu, W. lncRNA HLA Complex Group 18 (HCG18) Facilitated Cell Proliferation, Invasion, and Migration of Prostate Cancer Through Modulating miR-370-3p/DDX3X Axis. Reprod. Sci. 28, 3406–3416 (2021). https://doi.org/10.1007/s43032-021-00614-2

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