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Procyanidin B2 Improves Oocyte Maturation and Subsequent Development in Type 1 Diabetic Mice by Promoting Mitochondrial Function

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Abstract

Type 1 diabetes (T1D) results in decreased oocyte quality and compromised early embryonic development. Procyanidin B2 (PB2) is a natural compound extracted from grape seeds and has strong antioxidant activity in vivo. This study evaluated the effect of PB2 on oocyte maturation in diabetic mice. Diabetic mice were induced by streptozotocin (STZ) injection. PB2 was supplemented in the in vitro maturation medium, and the ratio of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE), reactive oxygen species (ROS) levels, mitochondrial function, developmental ability, as well as crotonylation at H4K5 were determined in oocytes. PB2 can promote the extrusion of PBE (88.34% vs. 75.02%, P < 0.05); reduce the generation of ROS (1.12 vs. 1.96, P < 0.05); and improve the level of mitochondrial membrane potential (0.87 vs. 0.79 Δφm, P < 0.05), ATP level (1.31 vs. 0.71 pmol, P < 0.05), and mitochondria temperature (618.25 vs. 697.39 pixels, P < 0.05). The addition of PB2 also improved the level of oocyte crotonylation at H4K5 (crH4K5) (47.26 vs. 59.68 pixels, P < 0.05) and increased the blastocyst rate (61.51% vs. 36.07%, P < 0.05) after parthenogenetic activation. Our results are the first to reveal a role for PB2 in promoting the viability of oocytes by regulating the mitochondrial function. Moreover, we uncover that PB2 can improve the level of crH4K5, which provides a new strategy to combat the decline in oocyte quality of diabetic.

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Acknowledgments

We thank Professor Yong-Tae Chang from POSTECH for giving the MTY. We thank Dr. Keren Cheng for giving the valuable suggestion concerning the manuscript.

Funding

This work was supported by the National Nature Science Foundation Project of China (No. 31101714, 81901562&31372307).

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Author notes

  1. Yuxi Luo and Qingrui Zhuan contributed equally to this work.

Authors and Affiliations

  1. National Engineering Laboratory for Animal Breeding, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China

    Yuxi Luo, Qingrui Zhuan, Xingzhu Du & Xiangwei Fu

  2. Department of Reproductive Medicine, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, Hebei, China

    Jun Li

  3. Chelsea and Westminster Hospital, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW10 9NH, UK

    Zhengyuan Huang

  4. State Key Laboratory of Agro biotechnology, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Rd 2, Haidian District, Beijing, 100193, China

    Yunpeng Hou

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  1. Yuxi Luo
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Contributions

This research was designed by Xiangwei Fu, Yuxi Luo, and Qingrui Zhuan. The in vitro maturation was performed by Yuxi Luo, the immunofluorescence staining (ROS, MT, H4K5 crotonylation) was performed by Yuxi Luo and Qingrui Zhuan. The cell culture and live imaging were performed by Yuxi Luo and Xingzhu Du. The obtained data were analyzed by Yuxi Luo and Xiangwei Fu. The results were interpreted by Jun Li, Zhengyuan Huang, and Yunpeng Hou. The manuscript was written by Yuxi Luo and Xiangwei Fu.

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Correspondence to Xiangwei Fu.

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This research did not involve human participants. All animal experiments were performed in compliance with the principles and guidelines for the use of laboratory animals and ratified by the Institutional Animal Care and Use Committee of China Agricultural University (AW01040202-1). This ethics statement was also mentioned in the manuscript.

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Luo, Y., Zhuan, Q., Li, J. et al. Procyanidin B2 Improves Oocyte Maturation and Subsequent Development in Type 1 Diabetic Mice by Promoting Mitochondrial Function. Reprod. Sci. 27, 2211–2222 (2020). https://doi.org/10.1007/s43032-020-00241-3

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