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Modulation of the Notch System in Response to Wnt Inhibition Induces Restoration of the Rat Luteal Function

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Abstract

The aim of this study was to investigate whether the Notch pathway is modulated in response to the downregulation of the Wnt/Β-catenin system in corpora lutea (CLs) from superovulated rats. To this end, we analyzed the effect of in vitro CL Wnt/Β-catenin inhibition on the expression of Notch members and on luteal function. Mechanically isolated rat CLs were cultured with ICG-001, a Wnt/B-catenin inhibitor. In this system, Wnt/B-catenin inhibition reduced progesterone production and decreased StAR protein levels. Besides, Wnt/B-catenin inhibition stimulated the Notch system, evidenced by an increase in Hes1 expression, and promoted the expression of selected Notch family members. At long incubation times, StAR levels and progesterone concentration reached the control values, effects probably mediated by the Notch pathway. These results provide the first evidence of a compensatory mechanism between Wnt/B-catenin signaling and the Notch system, which contributes to the homeostasis of luteal cells.

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Funding

This work was supported by Universidad de Buenos Aires (Grant number: UBA 01/Q502), Fondo para la Investigación Científica y Tecnológica (Grant number: PICT 2014-0429), Consejo Nacional de Investigaciones Científicas y Técnicas (Grant number: PIP 177), and Rene Baron Foundation, Argentina; Williams Foundation, Argentina.

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Author notes

  1. Paula Accialini and Andrés Bechis contributed equally to this work.

Authors and Affiliations

  1. Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina

    Paula Accialini, Andrés Bechis, Griselda Irusta, Maria Silvia Bianchi, Fernanda Parborell, Dalhia Abramovich & Marta Tesone

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  1. Paula Accialini
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  2. Andrés Bechis
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  7. Marta Tesone
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Correspondence to Marta Tesone.

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Accialini, P., Bechis, A., Irusta, G. et al. Modulation of the Notch System in Response to Wnt Inhibition Induces Restoration of the Rat Luteal Function. Reprod. Sci. 27, 503–512 (2020). https://doi.org/10.1007/s43032-019-00043-2

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  • DOI: https://doi.org/10.1007/s43032-019-00043-2

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