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An investigation of the possible methods and potential benefits of de novo cloning of Nannochloropsis oceanica genes

Marine Life Science & Technology volume 1, pages 22–27 (2019)Cite this article

Abstract

Species in the microalgal genus Nannochloropsis are increasingly used as models for theoretical and applied studies. Here we attempt to generate InDel variations in the genome of Nannochloropsis oceanica, and then decipher the genetic basis of its economic and biological traits with bulked mutant analysis modified from bulked segregant analysis. In addition, we describe our efforts to construct site-tagged and gene-traceable mutant libraries to clone its genes through reverse genetic approaches. Currently, more than a half of N. oceanica protein-encoding genes are annotated against databanks. However, no functional gene has been de novo cloned from N. oceanica and no new function has been assigned to any of its annotatable genes. Here, we discuss the possible methods and potential benefits of de novo cloning of N. oceanica genes.

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Acknowledgements

This study was financially supported by National Key R&D Program of China (2018YFD0900305 and 2018YFD0901506), The Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0406-3), and The Fundamental Research Funds for The Central Universities (201762017).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China (OUC), Qingdao, 266003, China

    Guanpin Yang, Zhongyi Zhang, Hang Liu & Li Guo

  2. Institutes of Evolution and Marine Biodiversity, OUC, Qingdao, 266003, China

    Guanpin Yang

  3. Key Laboratory of Marine Genetics and Breeding of Ministry of Education, OUC, Qingdao, 266003, China

    Guanpin Yang

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  1. Guanpin Yang
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  2. Zhongyi Zhang
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  3. Hang Liu
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  4. Li Guo
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Contributions

GY suggested the general idea of the context of manuscript. ZZ collected and reviewed the papers published early, which report the genetic manipulations of N. oceanica. HL collected and reviewed the papers published that report the strategies and methods either applicable or referable to the genetic studies of N. oceanica. LG drafted the manuscript.

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Correspondence to Li Guo.

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The authors declare no competing and conflicting interest.

Animal and human rights statement

The material reviewed here and used in our previous studies are a microalgal species. This species and plants are comparable. The associating statements are not applicable.

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Edited by Jiamei Li.

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Yang, G., Zhang, Z., Liu, H. et al. An investigation of the possible methods and potential benefits of de novo cloning of Nannochloropsis oceanica genes. Mar Life Sci Technol 1, 22–27 (2019). https://doi.org/10.1007/s42995-019-00014-1

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  • DOI: https://doi.org/10.1007/s42995-019-00014-1

Keywords

  • Nannochloropsis oceanica
  • Bulked mutant analysis
  • Mutant library
  • De novo cloning
  • Reverse genetics