Our previous study evaluated the in vitro and in vivo antioxidant activities of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme (HFPS). The results indicate that HFPS possesses potent antioxidant activity and suggest the potential use of HFPS to combat photoaging. In this study, we investigated the ultraviolet (UV) protective effect of HFPS in vitro in keratinocytes (HaCaT cells) and in vivo in zebrafish. The results indicate that HFPS significantly reduced the level of intracellular reactive oxygen species (ROS) and improved the viability of UVB-irradiated HaCaT cells. In addition, HFPS remarkably decreased apoptosis formation in UVB-irradiated HaCaT cells in a dose-dependent manner. The in vivo test results also demonstrate that HFPS significantly reduced intracellular ROS levels, cell death, NO production, and lipid peroxidation levels in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that HFPS possesses strong in vitro and in vivo UV-protective effects, making it a potential ingredient in the cosmeceutical industry.
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Ariede MB, Candido TM, Jacome ALM, Velasco MVR, De Carvalho JCM, Baby AR (2017) Cosmetic attributes of algae—a review. Algal Res 25:483–487
Cha S-H, Ko S-C, Kim D, Jeon Y-J (2011) Screening of marine algae for potential tyrosinase inhibitor: those inhibitors reduced tyrosinase activity and melanin synthesis in zebrafish. J Dermatol 38:354–363
Cha S-H, Ko C-I, Kim D, Jeon Y-J (2012) Protective effects of phlorotannins against ultraviolet B radiation in zebrafish (Danio rerio). Vet Dermatol 23:51–62
Dobashi K, Nishino T, Fujihara M, Nagumo T (1989) Isolation and preliminary characterization of fucose-containing sulfated polysaccharides with blood-anticoagulant activity from the brown seaweed Hizikia fusiforme. Carbohydr Res 194:315–320
Heo S-J, Jeon Y-J (2009) Protective effect of fucoxanthin isolated from Sargassum siliquastrum on UV-B induced cell damage. J Photochem Photobiol B Biol 95:101–107
Jeong SC, Jeong YT, Lee SM, Kim JH (2015) Immune-modulating activities of polysaccharides extracted from brown algae Hizikia fusiforme. Biosci Biotechnol Biochem 79:1362–1365
Ji D, You L, Ren Y, Wen L, Zheng G, Li C (2017) Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes. J Funct Foods 36:332–340
Kim Y-I, Oh W-S, Song PH, Yun S, Kwon Y-S, Lee YJ, Ku S-K, Song C-H, Oh T-H (2018) Anti-photoaging effects of low molecular-weight fucoidan on ultraviolet B-irradiated mice. Mar Drugs 16:286
Ko S-C, Cha S-H, Heo S-J, Lee S-H, Kang S-M, Jeon Y-J (2011) Protective effect of Ecklonia cava on UVB-induced oxidative stress: in vitro and in vivo zebrafish model. J Appl Phycol 23:697–708
Li B, Wei X-J, Sun J-L, Xu S-Y (2006) Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme. Carbohydr Res 341:1135–1146
Park HM, Moon E, Kim A-J, Kim MH, Lee S, Lee JB, Park YK, Jung H-S, Kim Y-B, Kim SY (2010) Extract of Punica granatum inhibits skin photoaging induced by UVB irradiation. Int J Dermatol 49:276–282
Ruocco N, Costantini S, Guariniello S, Costantini M (2016) Polysaccharides from the marine environment with pharmacological, cosmeceutical and nutraceutical potential. Molecules 21:551
Wang P, Zhao X, Lv Y, Liu Y, Lang Y, Wu J, Liu X, Li M, Yu G (2012) Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS. Carbohydr Polym 90:602–607
Wang L, Ryu B, Kim W-S, Kim GH, Jeon Y-J (2017) Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish. Algae 32:379–388
Wang L, Lee W, Oh JY, Cui YR, Ryu B, Jeon Y-J (2018a) Protective effect of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced skin damage by regulating NF-κB, AP-1, and MAPKs signaling pathways in vitro in human dermal fibroblasts. Mar Drugs 16:239
Wang L, Oh JY, Kim HS, Lee W, Cui Y, Lee HG, Kim Y-T, Ko JY, Jeon Y-J (2018b) Protective effect of polysaccharides from Celluclast-assisted extract of Hizikia fusiforme against hydrogen peroxide-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish. Int J Biol Macromol 112:483–489
Wang L, Park Y-J, Jeon Y-J, Ryu B (2018c) Bioactivities of the edible brown seaweed, Undaria pinnatifida: a review. Aquaculture 495:873–880
Wijesekara I, Pangestuti R, Kim S-K (2011) Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohydr Polym 84:14–21
Wijesinghe WAJP, Jeon YJ, Ramasamy P, Wahid MEA, Vairappan CS (2013) Anticancer activity and mediation of apoptosis in human HL-60 leukaemia cells by edible sea cucumber (Holothuria edulis) extract. Food Chem 139:326–331
Zaid MA, Afaq F, Syed DN, Dreher M, Mukhtar H (2007) Inhibition of UVB-mediated oxidative stress and markers of photoaging in immortalized HaCaT keratinocytes by pomegranate polyphenol extract POMx. Photochem Photobiol 83:882–888
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1A6A1A03033553).
Conflict of interest
The authors declare that they have no conflict of interest.
We declare that all applicable international, national, and/or institutional guidelines for sampling, care, and experimental use of organisms for the study have been followed and all necessary approvals have been obtained.
Edited by Xin Yu.
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Wang, L., Oh, J.Y., Yang, HW. et al. Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish. Mar Life Sci Technol 1, 104–111 (2019). https://doi.org/10.1007/s42995-019-00006-1
- Hizikia fusiforme
- Sulfated polysaccharides
- Ultraviolet B