The European polecat (Mustela putorius) almost became extinct in Britain in the early twentieth century, but populations are now recovering. As seen in other endangered carnivore populations, disease is one potential threat to recovery. This study assessed exposure of wild polecats (n = 149) to three, multi-host pathogens which could limit reproduction and/or cause morbidity and mortality. Serum, lung and brain samples were collected from polecats which died from 2011 to 2016 across Britain. Exposure to Toxoplasma gondii and 12 Leptospira serovars was assessed serologically by antibody detection using the latex agglutination test and microscopic agglutination test, respectively, and the presence of canine distemper virus (CDV) RNA in lung and brain tissue samples was assessed using PCR. Generalised linear models were used to test for relationships between exposure to each pathogen and season, sex, age, and location. All organ samples tested PCR negative for CDV (95% CI 0.00–0.05%). There was evidence of frequent exposure to T. gondii with a recorded seroprevalence of 71.8% (95% CI 64.2–79.4%) and moderate exposure to Leptospira serovars, 14.5% (95% CI 8.6–20.4%). Season, sex, age, and location were not significantly associated with exposure to T. gondii or Leptospira serovars. Evidence of exposure to T. gondii and Leptospira serovars in European polecats could potentially affect mortality, longevity or fecundity. Further studies are warranted to assess the impact of these pathogens on polecat populations in Britain.
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Alexander KA, McNutt JW, Briggs MB, Standers PE, Funston P, Hemson G, Keet D, van Vuuren M (2010) Multi-host pathogens and carnivore management in southern Africa. Comp Immunol Microbiol Infect Dis 33(3):249–265
Barros M, Cabezón O, Dubey J, Almería S, Puig Ribas M, Escobar LE, Ramos B, Medina Vogel G (2018) Toxoplasma gondii infection in wild mustelids and cats across an urban-rural gradient. PLoS ONE 13(6):e0199085
Birks J (2015) Polecats the British natural history collection, vol 5. Whittet Books Ltd, Essex, pp 82–97
Birks JDS (1998) Secondary rodenticide poisoning risk arising from winter farmyard use by the European polecat Mustela putorius. Biol Cons 85:233–240
Birks JDS (2008) The Polecat Survey of Britain 2004–2006. A report on the Polecat's distribution, status and conservation. The Vincent Wildlife Trust, Ledbury
Burns R, Williams ES, O'Toole D, Dubey JP (2003) Toxoplasma gondii infections in captive black-footed ferrets (Mustela nigripes) 1992–1998: clinical signs, serology, pathology, and prevention. J Wildl Dis 39(4):787–797
Burrells A, Bartley PM, Zimmer IA, Roy S, Kitchener AC, Meredith A, Wright SE, Innes EA, Katzer F (2013) Evidence of the three main clonal Toxoplasma gondii Lineages from wild mammalian carnivores in the UK. Parasitology 140(14):1768–1776
Costa M, Fernandes C, Birks JDS, Kitchener AC, Santos-Reis M, Bruford M (2013) The genetic legacy of the 19th-century decline of the British polecat: evidence for extensive introgression from feral ferrets. Mol Ecol 22(20):5130–5147
Croose E (2016) The distribution and status of the polecat (Mustela putorius) in Britain 2014–2015. The Vincent Wildlife Trust Ledbury
Delahay R, Frölich K (2000) Absence of antibodies against canine distemper virus in free-ranging populations of the Eurasian badger in great Britain. J Wildl Dis 36(3):576–579
Ellis WA (2015) Animal leptospirosis. In: Adler B (ed) Leptospira and leptospirosis. Current topics in microbiology and immunology, Springer, Berlin, Heidelberg, pp 99–137
Frank RK (2001) An outbreak of toxoplasmosis in farmed mink (Mustela vison S.). J Vet Diagn Invest 13(3):245–249
Harrington LA, Gelling M, Simpson V, Harrington A, Macdonald DW (2012) Notes on the health status of free-living, non-native American mink, Neovison vison, in southern England. Eur J Wildl Res 58(5):875–880
Hollings T, Jones M, Mooney N, McCallum H (2013) Wildlife disease ecology in changing landscapes: mesopredator release and toxoplasmosis. Int J Parasitol Paras Wildl 2:110–118
Horton NJ, Kleinman K (2015) Using R and RStudio for data management, statistical analysis, and graphics, 2nd edn. Chapman and Hall/CRC, Boca Raton
Hughes JM, Thomasson D, Craig PS, Georgin S, Pickles A, Hide G (2008) Neospora caninum: Detection in wild rabbits and investigation of co-infection with Toxoplasma gondii by PCR analysis. Exp Parasitol 120(3):255–260
Johnson N, Mansfield KL, Marston DA, Wilson C, Goddard T, Selden D, Hemson G, Edea L, van Kesteren F, Shiferaw F, Stewart AE, Sillero−Zubiri C, Fooks AR (2010) A new outbreak of rabies in rare Ethiopian wolves (Canis simensis). Arch Virol 155(7):1175–1177
Kiupel M, Perpiñán D (2014) Viral diseases of ferret biology and diseases of the ferret, 3rd edn. Wiley, Ames, pp 439–517
Langley PJW, Yalden DW (1977) The decline of the rarer carnivores in Great Britain during the nineteenth century. Mammal Rev 7:95–116
Mathews F, Kubasiewicz M, Gurnell J, Harrower C, McDonald R, Shore R (2018) A review of the population and conservation status of British Mammals: technical summary. Natural, England, Peterborough
Matson GM, Van Daele L, Goodwin E, Aumiller L, Reynolds H, Hristienko H (1993) A laboratory manual for cementum age determination of Alaska Brown Bear PM1 teeth. Matson's Lab, Milltown, Montana
Moinet M, Fournier-Chambrillon C, André-Fontaine G, Aulagnier S, Mesplède A, Blanchard B, Descarsin V, Dumas P, Dumas Y, Coïc C, Couzi L, Fournier P (2010) Leptospirosis in free-ranging endangered European Mink (Mustela lutreola) and other small carnivores (mustelidae, viverridae) from SouthWestern France. J Wildl Dis 46(4):1141–1151
Sainsbury KA, Shore RF, Schofield H, Croose E, Pereira MG, Sleep D, Kitchener AC, Hantke G, McDonald RA (2018) Long-term increase in secondary exposure to anticoagulant rodenticides in European polecats Mustela putorius in Great Britain. Environ Pollut 236:689–698
Sainsbury KA, Shore RF, Schofield H, Croose E, Campbell RD, McDonald RA (2019) Recent history, current status, conservation and management of native mammalian carnivore species in Great Britain. Mammal Rev 49(2):171–188
Sainsbury KA, Shore RF, Schofield H, Croose E, Hantke G, Kitchener AC, McDonald RA (2020) Diets of European polecat Mustela putorius in Great Britain during fifty years of population recovery. Mammal Res 65(2):181–190
Sato JJ, Hosoda T, Wolsan M, Tsuchiya K, Yamamoto M, Suzuki H (2003) Phylogenetic relationships and divergence times among mustelids (Mammalia: Carnivora) based on nucleotide sequences of the nuclear interphotoreceptor retinoid binding protein and mitochondrial cytochrome b genes. Zool Sci 20(2):243–264
SAVSNET (2018) SAVSNET in realtime. https://www.liverpool.ac.uk/savsnet/real-time-data/. Accessed 23 May 2018
Schuller S, Francey T, Hartmann K, Hugonnard M, Kohn B, Nally JE, Sykes J (2015) European consensus statement on leptospirosis in dogs and cats. J Small Anim Pract 56(3):159–179
Sepúlveda MA, Muñoz-Zanzi C, Rosenfeld C, Jara R, Pelican KM, Hill D (2011) Toxoplasma gondii in feral American minks at the Maullín river, Chile. Vet Parasitol 175(1–2):60–65
Sobrino R, Cabezón O, Millán J, Pabón M, Arnal MC, Luco DF, Gortázar C, Dubey JP, Almeria S (2007) Seroprevalence of Toxoplasma gondii antibodies in wild carnivores from Spain. Vet Parasitol 148(3–4):187–192
Soulsbury CD, Iossa G, Baker PJ, Cole NC, Funk SM, Harris S (2007) The impact of sarcoptic mange Sarcoptes scabiei on the British fox Vulpes vulpes population. Mammal Rev 37(4):278–296
Swennes AG, Fox JG (2014) Bacterial and mycoplasmal diseases biology and diseases of the ferret, 3rd edn. Wiley, Ames, pp 519–552
Thorne ET, Williams ES (1988) Disease and endangered species: the black-footed ferret as a recent example. Conserv Biol 2(1):66–74
Thornton RN, Cook TG (1986) A congenital Toxoplasma-like disease in ferrets (Mustela putorius furo). NZeal Veterin J 34(3):31–33
Twigg GI (2008) A review of the occurrence in British mammals of the major organisms of zoonotic importance. Mammal Rev 10(4):139–149
Webster JP (2001) Rats, cats, people and parasites: the impact of latent toxoplasmosis on behaviour. Microbes Infect 3(12):1037–1045
Webster JP, Ellis WA, Macdonald DW (1995) Prevalence of Leptospira spp. in wild brown rats (Rattus norvegicus) on UK farms. Epidemiol Infect 114(1):195–201
The Vincent Wildlife Trust supported the study by providing the polecat specimens used in this research. The National Museums Scotland supported the study by collecting the blood and tissue samples, and ACK is grateful to the Negaunee Foundation for their continuing generous support of a preparatory at the National Museums Scotland. Veterinary Diagnostic Services at the University of Glasgow and the Leptospirosis laboratory at Agri-Food and Biosciences Institute Stormont Belfast carried out the serological assays reported in this study and the Animal and Plant Health Agency carried out the CDV PCR testing. The authors thank Colm Gilmore for his advice on the Leptospira serovar assay and Professor Brian Willet for advice on CDV serological assays. Matson’s Laboratory LLC USA supported the study by ageing the specimens. KAS was supported by the University of Exeter, Vincent Wildlife Trust and Centre for Ecology & Hydrology.
The diagnostic testing was supported by Dr. Johnny Birks, The Blodwen Lloyd Binns Bequest Fund of the Glasgow Natural History Society and The Zebra Foundation of the British Veterinary Zoological Society. The teeth ageing analysis was funded by a Peoples’ Trust for Endangered Species grant. The funders’ sole role for the project was to support it financially.
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Heald, KA., Millins, C., Kitchener, A.C. et al. Investigating infectious disease threats to the recovery of the European polecat in Britain. Mamm Biol 100, 439–444 (2020). https://doi.org/10.1007/s42991-020-00046-6
- Canine distemper virus
- Mustela putorius
- Toxoplasma gondii