Abstract
An in vitro pollen germination protocol for Triticale, the man-made cereal, has been reported. Triticale pollen may be classified as recalcitrant pollen as it is difficult to germinate on artificial medium. Using this pollen germination medium (PGM), viability and pollen vigour of triticale lines may be tested within 10–15 min. PGM used for triticale consisted of 19% maltose, 15% polyethylene glycol 4000, 60 mgl−1 boric acid, 20 mgl−1 calcium nitrate, 80 mgl−1 potassium nitrate, 160 mgl−1 magnesium sulphate and 1% agar. This PGM has been altered slightly which supported pollen germination of other genotypes. This complex medium contains many inorganic salts but in low concentration. The PGM showed > 93% pollen germination with intact pollen tubes at 17 ± 1 °C and pH 6.8. During the flowering period, a pollen sterility of less than 10% was recorded. Triticale pollen was viable more than 1.45 h which is much longer time as compared to wheat. This tool would be useful in triticale breeding especially in wide crosses with wheat or rye.
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PJ involved in conceptualization of research; PJ involved in designing of the experiments; PJ, VKV, and MS involved in contribution of experimental materials; PJ, PJ, PS, RN, VB, and MG involved in execution of field/laboratory experiments and data collection; PJ and PJ involved in analysis of data and interpretation; PJ, VKV, and MS involved in preparation of the manuscript.
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Communicated by J. Zimny.
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42976_2022_275_MOESM2_ESM.jpg
SM2: Photomicrograph showing triticale pollen germination of three genotypes (A) Coorong (B) TL 2969 (C) TL 2942 after 15 min after culture: Scale bar: 9.73µm (JPG 65 kb)
SM1 A-C .mpeg: Video clips showing triticale in vitro pollen germination (ZIP 13535 kb)
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Jayaprakash, P., Peter, J., Shajitha, P. et al. Development of in vitro pollen germination protocol for recalcitrant triticale pollen (X Triticosecale Wittmack). CEREAL RESEARCH COMMUNICATIONS 51, 189–196 (2023). https://doi.org/10.1007/s42976-022-00275-0
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DOI: https://doi.org/10.1007/s42976-022-00275-0