Abstract
Four functional component content QTLs of brown rice were detected by association mapping with a total of 309 pairs of SSR markers using the Yunnan mini-core collection and its near-isogenic lines (BC6F6) in this study. There were 52 marker loci associated with resistant starch, total flavonoids, gamma-aminobutyric acid and alkaloid content in brown rice. In the mini-core collection, 13 marker loci associated with four functional component content . RM6054, GXRc , RM6063 and RM547 were detected for repetition within two years. RM6054 and RM6063 were markers associated with resistant starch content, and GXRc and RM547 were associated with total flavonoids content. In all, 41 markers were associated with four functional component contents in brown rice in the BC6F6 population, 23 of which, were detected by repetition. These marker loci contained some new QTL sites associated with functional components contents. Additionally, some of the detected markers were associated with multiple functional components of brown rice in the study. Moreover, correlation analysis of the four functional components revealed significant correlation between total flavonoids and resistant starch, gamma-aminobutyric acid and alkaloids. These results indicate that the synthesis of the four functional components may be correlated and provide new clues to reveal the genetic basis of their synthesis of the four functional components in brown rice. Furthermore, some important QTL regions may facilitate future identification of the genes related to the four functional components.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (31760376), Science and Technology Planning Project of Yunnan science and technology department (2018FB059).
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XL, XY, JD, ZC and MW contributed equally to this study. Conceptualization, XL and XY; Formal analysis, XL and ZC; Investigation, JD and MW; Methodology, ZC and MW; Resources, YW; Supervision, YW, JY, XP, LC; Visualization, XL and XY; Writing-original draft, XL and XY; Writing-review& editing, TY and MS.
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Communicated by A. Börner.
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42976_2021_191_MOESM1_ESM.jpg
FigureS1 Manhattan diagram of association analysis with RS content. Note: (A) shows the Manhattan diagram made by association analysis of mini-core collections in 2016 with RS content, (B) shows the Manhattan diagram made by association analysis in 2017 with RS content. In the red circle contains the association loci repeatedly detected in the two years of study (P value<0.01). (JPG 791 kb)
42976_2021_191_MOESM2_ESM.jpg
FigureS2 Manhattan diagram of association analysis with flavonoid content. Note: (A) shows the Manhattan diagram made by association analysis of mini-core collections with flavonoid content in 2016, (B) shows the Manhattan diagram made by the same association analysis in 2017. In the red circle contains the association loci repeatedly detected in the two years of study (P value<0.01). (JPG 1003 kb)
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Li, X., Yang, X., Du, J. et al. Identification of four functional component content QTLs of brown rice in the Yunnan mini-core collection and its near-isogenic lines using association mapping. CEREAL RESEARCH COMMUNICATIONS 50, 357–366 (2022). https://doi.org/10.1007/s42976-021-00191-9
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DOI: https://doi.org/10.1007/s42976-021-00191-9