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Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil

  • Clinical Microbiology - Research Paper
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Abstract

Klebsiella pneumoniae strains are globally associated with a plethora of opportunistic and severe human infections and are known to spread genes conferring antimicrobial resistance. Some strains harbor virulence determinants that enable them to cause serious disease in any patient, both in the hospital and in the community. The aim of this study was to determine the frequency of antimicrobial resistance and virulence traits (by gene detection and string test) among 83 K. pneumoniae isolates obtained from patient cultures of a scholar tertiary hospital in the Midwestern Brazil (Brasília, DF). Antimicrobial susceptibility analysis showed that 94% (78/83) of the isolates presented one of the following resistance profiles: resistant (R, 39), multidrug-resistant (MDR, 29), or extensively drug-resistant (XDR, 10). Several MDR and XDR strains harbored multiple virulence genes and displayed hypermucoviscous phenotype. These characteristics were observed among isolates obtained throughout all the sample collection period (2013 – 2017). The K2 serotype gene, a molecular marker of hypervirulence, was detected in three isolates, one of which classified as XDR. Sequence typing revealed the occurrence of isolates belonged to high-risk (ST13) and multiple resistance-spreading clones (ST105). Thus, our findings showed the occurrence of virulent potential isolates that also presented MDR/XDR phenotypes from 2013 to 2015. This study also indicates the probable convergence of virulence and resistance since at least 2013 in Brazil.

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Funding

This work was supported by the São Paulo State Research Support Foundation (FAPESP) (Grant no. 2013 / 22581–5).

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação Em Biologia Microbiana, Universidade de Brasília, Brasília, Distrito Federal, Brasil

    Ana Paula Cardoso Almeida & Tatiana Amabile de Campos

  2. Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Brasilia, DF, CEP70910-900, Brazil

    Ana Paula Cardoso Almeida, Georgios Joannis Pappas Junior & Tatiana Amabile de Campos

  3. Faculdade de Ciências Farmacêuticas, Universidade de Ribeirão Preto, Ribeirão Preto, São Paulo, Brasil

    Miguel Augusto de Moraes, Amanda Kamyla Ferreira da Silva & André Pitondo-Silva

  4. Programa de Pós-Graduação Em Tecnologia Ambiental, Universidade de Ribeirão Preto, Ribeirão Preto, São Paulo, Brasil

    Miguel Augusto de Moraes, Amanda Kamyla Ferreira da Silva, Mariana Oliveira-Silva, Rafael Nakamura-Silva & André Pitondo-Silva

  5. Programa de Pós-Graduação Em Odontologia, Universidade de Ribeirão Preto, Ribeirão Preto, São Paulo, Brasil

    André Pitondo-Silva

  6. Programa de Pós-Graduação Em Biologia Molecular, Universidade de Brasília, Brasília, DF, Brasil

    Felipe Marques de Almeida & Georgios Joannis Pappas Junior

Authors
  1. Ana Paula Cardoso Almeida
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  2. Miguel Augusto de Moraes
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  3. Amanda Kamyla Ferreira da Silva
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  4. Mariana Oliveira-Silva
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  6. Felipe Marques de Almeida
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  7. Georgios Joannis Pappas Junior
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  8. André Pitondo-Silva
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  9. Tatiana Amabile de Campos
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Contributions

TAC e APS designed this study, contributed to the data analysis, write, and review the manuscript. APC isolated the bacteria and write the manuscript. AKFS performed antimicrobial susceptibility tests, MAM, MOS, RNS carried out experiments related to PCR and hypermucoviscosity. FMA and GJ.

Corresponding author

Correspondence to Tatiana Amabile de Campos.

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Ethics statement

The studies involving human participants were reviewed and approved by the ethical from the Faculdade de Medicina, Universidade de Brasília, Brasília, DF, Brazil (approval no. CEP/FMUnB 1.131.054; CAEE: 44867915.1.0000.558).

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The authors declare no competing interests.

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Responsible Editor: Cristiano Gallina Moreira

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Cardoso Almeida, A.P., de Moraes, M.A., da Silva, A.K.F. et al. Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01358-2

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