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Eugenia uniflora (pitanga) juice as a new alternative vehicle for Limosilactobacillus fermentum ATCC 23271: evaluation of antioxidant and anti-infective effects

  • Biotechnology and Industry - Short Communication
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Abstract

Probiotic-containing foods are among the most appreciated functional foods; however, probiotic-based dairy products cannot be consumed by people who are lactose intolerant, allergic to milk, or vegetarian or vegan individuals. Thus, new non-dairy matrices have been tested for probiotics delivery. This study evaluated the growth and viability of Limosilactobacillus fermentum ATCC 23271 and Lacticaseibacillus rhamnosus ATCC 9595 in Pitanga juice (Eugenia uniflora L.). The effects of the fermentation on the antioxidant and anti-infective properties of the juice were also analyzed. The E. uniflora juice allowed lactobacilli growth without supplementation, reaching rates around 8.4 Log CFU/mL and producing organic acids (pH values < 4) after 72 h of fermentation. The strain remained viable after 35 days of refrigerated storage. Fermentation by these bacteria increases the antioxidant capacity of the juice. The central composite rotational design was employed to evaluate the effects of bacterial inoculum and pulp concentration on growth and organic acids production by L. fermentum ATCC 23271. The strain was viable and produced organic acids in all tested combinations. L. fermentum-fermented juice and its cell-free supernatant significantly increased the survival of Tenebrio molitor larvae infected by enteroaggregative Escherichia coli 042. The results obtained in this study provide more insights into the potential of Pitanga juice to develop a functional non-dairy probiotic beverage with antioxidant and anti-infective properties.

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Data availability

Data are available upon request.

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Acknowledgements

The authors would like to thank the staff of LACEN-MA.

Funding

This work was funded by Fundação de Amparo à Pesquisa e Desenvolvimento Científico do Maranhão (Processes numbers: POS-GRAD-02460/21, INFRA-02032/21; ACT 05691/21 FAPEMA/CAPES), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant number: 312349/2020-3).

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Authors and Affiliations

  1. Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil

    Gabrielle Damasceno Costa dos Santos, Carlos Drielson da Silva Pereira, Marcos Andrade Silva, Lucas dos Santos Silva, Simeone Júlio dos Santos Castelo Branco, Rita de Cássia Mendonça de Miranda & Luís Cláudio Nascimento da Silva

  2. Laboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, 65075-120, MA, Brazil

    Camila Caetano da Silva & Adrielle Zagmignan

  3. Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil

    Brenda da Silva Cutrim, Patrícia Maria Guedes Paiva & Maria Tereza dos Santos Correia

Authors
  1. Gabrielle Damasceno Costa dos Santos
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  2. Carlos Drielson da Silva Pereira
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  3. Camila Caetano da Silva
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  12. Luís Cláudio Nascimento da Silva
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Contributions

Gabrielle Damasceno Costa dos Santos: Conceptualization, Methodology, Investigation, Writing-Original draft preparation; Carlos Drielson da Silva Pereira: Writing-Original draft preparation; Camila Caetano da Silva: Investigation; Marcos Andrade Silva: Investigation; Lucas dos Santos Silva: Investigation; Brenda da Silva Cutrim: Investigation; Simeone Júlio dos Santos Castelo Branco: Investigation; Patrícia Maria Guedes Paiva: Supervision, Funding acquisition; Maria Tereza dos Santos Correia: Supervision, Funding acquisition; Rita de Cássia Mendonça de Miranda: Supervision, Investigation; Adrielle Zagmignan: Conceptualization, Supervision, Methodology, Funding acquisition; Luís Cláudio Nascimento de Silva: Conceptualization, Supervision, Methodology, Funding acquisition, Writing- Reviewing and Editing.

Corresponding author

Correspondence to Luís Cláudio Nascimento da Silva.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Costa dos Santos, G.D., da Silva Pereira, C.D., da Silva, C.C. et al. Eugenia uniflora (pitanga) juice as a new alternative vehicle for Limosilactobacillus fermentum ATCC 23271: evaluation of antioxidant and anti-infective effects. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01329-7

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