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Molecular detection of ovine gammaherpesvirus 2 in free ranging wild boars (Sus scrofa) from Southern Brazil

  • Veterinary and Animal Production - Research Paper
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Abstract

Ovine gammaherpesvirus 2 (OvGHV2) is a member of Macavirus genus, subfamily Gammaherpesvirinae, family Herpesviridae, and causes sheep associated-malignant catarrhal fever (SA-MCF) in a wide range of ungulates. However, no descriptions of SA-MCF and/or infections due to OvGHV2 were identified in the wild boar (Sus scrofa). This study investigated the occurrence of OvGHV2 in the lungs (n = 44) of asymptomatic, free ranging wild boars captured in several regions of Paraná State, Southern Brazil. A PCR assay targeting the OvGHV2 tegument protein gene amplified OvGHV2 DNA in 4.55% (2/44) of the pulmonary tissues evaluated. Sequence analysis confirmed that the OvGHV2 strains herein identified have 98.4% deduced amino acid (aa) sequence identity with the prototype strain of OvGHV2 and 96.4–100% aa identity with similar strains of OvGHV2 detected in several animal species from diverse countries. These findings confirmed that these two wild boars were infected by OvGHV2, represent the first description of this infection in these animals, and add to the number of pathogens identified in this animal species. Furthermore, these findings contrast earlier descriptions of OvGHV2 in swine since in all previous reports the infected pigs demonstrated clinical manifestations of disease. Consequently, these wild boars from Southern Brazil were subclinically infected or suffered asymptomatic infections by OvGHV2.

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Data availability

The OvGHV2 sequences derived from this study are deposited in GenBank https://www.ncbi.nlm.nih.gov/genbank/ (Accession # OQ511653 and OQ511654).

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Acknowledgements

Headley, S.A., Alfieri, F.A. and Alfieri, A.A are recipients of the Brazilian National Council of Scientific and Technological Development (CNPq; Brazil) fellowships.

Funding

Brazilian National Council of Scientific and Technological Development (CNPq; Brazil); Coordination for the Improvement of Higher Education Personnel (CAPES; Brazil); National Institute of Science and Technology of Dairy Production Chain (INCT‑Leite).

Conselho Nacional de Desenvolvimento Científico e Tecnológico

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Authors and Affiliations

  1. Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil

    Selwyn Arlington Headley

  2. Multi‑User Animal Health Laboratory (LAMSA), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil

    Selwyn Arlington Headley, Alice Fernandes Alfieri & Amauri Alcindo Alfieri

  3. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil

    Carolina Yuka Yasumitsu, Alais Maria Dall Agnol, Tatiana Carolina Gomes Dutra de Souza, Gisele da Silva Porto, Raquel Arruda Leme, Alice Fernandes Alfieri & Amauri Alcindo Alfieri

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Correspondence to Selwyn Arlington Headley.

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This study was approved by the Ethics Committee on Animal Experiments of the Universidade Estadual de Londrina (# 22831.2017.40).

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Headley, S.A., Yasumitsu, C.Y., Dall Agnol, A.M. et al. Molecular detection of ovine gammaherpesvirus 2 in free ranging wild boars (Sus scrofa) from Southern Brazil. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01302-4

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