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High-risk clones of carbapenem resistant Klebsiella pneumoniae recovered from pediatric patients in Southern Brazil

  • Clinical Microbiology - Short Communication
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Abstract

Carbapenem-resistant Klebsiella pneumoniae (CRKP) exhibit high mortality rates in pediatric patients and usually belong to international high-risk clones. This study aimed to investigate the molecular epidemiology and carbapenem resistance mechanisms of K. pneumoniae isolates recovered from pediatric patients, and correlate them with phenotypical data. Twenty-five CRKP isolates were identified, and antimicrobial susceptibility was assessed using broth microdilution. Carbapenemase production and β-lactamase genes were detected by phenotypic and genotypic tests. Multilocus sequence typing was performed to differentiate the strains and whole-genome sequencing was assessed to characterize a new sequence type. Admission to the intensive care unit and the use of catheters were significantly positive correlates of CRKP infection, and the mortality rate was 36%. Almost all isolates showed multidrug-resistant phenotype, and most frequent resistant gene was blaKPC. We observed the dissemination of ST307 and clones belonging to CG258, which are considered high risk. In pediatric patients, these clones present with high genomic plasticity, favoring adaptation of the KPC and NDM enzymes to healthcare environments.

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Fig. 1

Data Availability

The draft genome of Kpn10 was submitted to GenBank under the accession number JASEKY000000000.2.

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Acknowledgements

We thank the Institute Pasteur teams for the curation and maintenance of BIGSdb-Pasteur databases at https://bigsdb.pasteur.fr/, and the Centro de Diagnóstico Avançado Pequeno Príncipe (CDAPP) for Sanger Sequencing.

Funding

This study was financed in part by the Fundação Araucária, and CEDCA PR – Conselho Estadual dos Direitos da Criança e do Adolescente do Paraná. The funders had no role in the study design, data collection and interpretation, or decision to submit this manuscript for publication.

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Authors and Affiliations

  1. Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil

    Damaris Krul, Adriele Celine Siqueira, Dany Mesa, Érika Medeiros dos Santos, Thaís Muniz Vasconcelos, Regiane Nogueira Spalanzani, Danieli Conte & Libera Maria Dalla-Costa

  2. Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 –Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil

    Damaris Krul, Luiza Souza Rodrigues, Adriele Celine Siqueira, Dany Mesa, Érika Medeiros dos Santos, Thaís Muniz Vasconcelos, Regiane Nogueira Spalanzani, Danieli Conte & Libera Maria Dalla-Costa

  3. Hospital Pequeno Príncipe (HPP), Curitiba, Paraná, Brazil

    Érika Medeiros dos Santos, Marinei Campos Ricieri & Fábio de Araújo Motta

  4. 4Biomics.Com, Curitiba, Paraná, Brazil

    Rodrigo Cardoso

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  1. Damaris Krul
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Contributions

Conceptualization: L.S.R., F.A.M., L.M. D-C.; Methodology: D.K., L.S.R., A.C.S., É. M.S., T.M.V., R.N.S.; Formal analysis and investigation: D.K., L.S.R, A.C.S., D.M., R.C., M.C.R., F.A.M., D.C.; Funding acquisition: L.M.D-C. Writing—original draft preparation: D.K., D.C.; Writing—review and editing: D.K, L.S.R., D.M., D.C., L.M.D-C; Supervision: L.S.R., D.C., L.M.D-C.

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Correspondence to Libera Maria Dalla-Costa.

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Krul, D., Rodrigues, L.S., Siqueira, A.C. et al. High-risk clones of carbapenem resistant Klebsiella pneumoniae recovered from pediatric patients in Southern Brazil. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01299-w

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