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Shifts in microbiota and gene expression of nutrient transporters, mucin and interleukins in the gut of fast-growing and slow-growing chickens infected by Salmonella Enteritidis

  • Veterinary Microbiology - Research Paper
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Abstract

Slow-growing breeds are more resistant to Salmonella infection compared to fast-growing broilers. However, it is unclear whether that is associated with innate resistance or rather rely on differences in Salmonella-induced gut responses. We investigated the microbial composition and gene expression of nutrient transporters, mucin, and interleukin in the gut of a fast-growing (Cobb500) and a slow-growing naked neck (NN) chicken breeds challenged with Salmonella Enteritidis. Hatchlings were inoculated at two days of age using sterile broth (sham) or Salmonella Enteritidis (SE) and distributed according to a completely randomized design into four treatments: Cobb-sham; Cobb-SE; NN-sham; and NN-SE. Cecal SE counting and microbial composition by 16 S rRNA sequencing were determined at 24-, 96-, and 168-hours post-inoculation (hpi). Gene expression of amino acid (Asct1) and peptide transporters (PepT1), glucose transporters (Sglt1, Glut2 and Glut5) and mucin (Muc2) in the jejunum and expression of interleukins (IL1 beta, IL8, IL17 and IL22) in the cecum was assessed by qPCR at 24 and 168 hpi. NN birds were colonized by SE just as Cobb birds but showed innate upregulation of Muc2, IL8 and IL17 in comparison to Cobb. While nutrient transporter mRNA expression was impaired in SE-challenged Cobb birds, the opposite was observed in NN. There were no differences in microbial diversity at different sampling times for Cobb-SE, whereas the other groups had higher diversity and lower dominance at 24 hpi compared with 96 hpi and 168 hpi. NN birds apparently develop earlier gut microbial stability, have higher basal level of mucin gene expression as well as differential nutrient transporter and interleukin gene expression in the presence of SE which might mitigate the effects of SE infection compared to Cobb birds.

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The datasets generated during and/or analyzed during the current study are not publicly available but can be made available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Finance code 001) for scholarships granted to MRBS, ALBMF, MFSA, NVMS, GFCS and MLPL; Conselho Nacional de Pesquisa e Desenvolvimento (CNPq) for scholarship (311860/2016-8) and research funding granted to PENG (458340/2014-6) and Financiadora de Estudos e Projetos (FINEP) for equipment supply in the scope of MCT-Infra institutional projects.

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Authors and Affiliations

  1. Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB, 58397-000, Brazil

    Maylane Rayane Brito dos Santos, Maria de Fátima de Souza Andrade, Gustavo Felipe Correia Sales, Celso José Bruno de Oliveira & Patrícia Emília Naves Givisiez

  2. Departamento de Ciência Animal, Centro de Ciências Humanas Sociais e Agrárias, Universidade Federal da Paraíba (UFPB), Bananeiras, PB, 58220-000, Brazil

    Alexandre Lemos de Barros Moreira Filho

  3. Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, 31710-220, Brazil

    Oliveiro Caetano Freitas Neto

  4. Instituto Federal do Sertão Pernambucano (IF-PE), BR-232, Km 508, s/n - Zona Rural, Salgueiro, PE, 56000-000, Brazil

    Núbia Michelle Vieira da Silva

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  1. Maylane Rayane Brito dos Santos
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Contributions

ALBMF, PENG, MFSA and CJBO conceived and designed research. MRBS, MFSA, NMVS, MLPL, GFCS conducted experiment, laboratorial and bioinformatic analyses. CJBO and OCFN contributed with reagents. MRBS, PENG, ALBMF, OCFN, MFSA, NMVS, GS, MLPL and CJBO analyzed data. MRBS, PENG, CJBO and GFCS wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Patrícia Emília Naves Givisiez.

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All management practices, as well as slaughter and sampling procedures were approved ty the Ethical Committee for the use of Animals from Universidade Federal da Paraiba (protocol 186/15) in compliance with the National Council for Animal Experimentation Control – CONCEA [Conselho Nacional de Controle de Experimentação Animal - CONCEA] (Federal Law nº 11.794/08, Lei Arouca) as established in art. 225 of the Brazilian National Constitution on the guidance for the use of animals for scientific purposes.

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Santos, M.R.B., Moreira Filho, A.L., Freitas Neto, O.C. et al. Shifts in microbiota and gene expression of nutrient transporters, mucin and interleukins in the gut of fast-growing and slow-growing chickens infected by Salmonella Enteritidis. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01297-y

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