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Enhanced laccase activity in Trametes versicolor (L.: Fr.) Pilát by host substrate and copper

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Laccases are appealing biocatalysts for various industrial utilizations. The fungus Trametes versicolor (L.: Fr.) Pilát causes white rot in wood and has been identified as an important fungal laccase producer. To investigate laccase production and activity in T. versicolor, the native isolate was collected from the host (Quercus castaneifolia) in the forests of Guilan province, northern Iran, and then purified and identified using the molecular marker. Its ability to produce laccase enzyme in the presence of different plant substrates including sawdust and wood chips of oak, poplar, and pine was evaluated. Also, the effect of copper as an enzyme inducer was investigated in vitro. The results showed that adding the wood to the culture medium increased laccase production, and among these, oak sawdust had the greatest effect, a 1.7-fold increase from that in the control (4.8 u/l vs. 2.8 u/l). Also, the enzyme extraction time effect on the optimal recovery yield showed that the 5-h enzyme extraction cycle resulted in the highest yield of the enzyme (18.97 u/l). Moreover, adding different concentrations of copper to the fungal culture medium increased the production of laccase, and the highest amount of enzyme (92.04 u/l) was obtained with 3.5 mM of CuSO4 along with oak sawdust. Based on the results, the addition of host wood sawdust (“oak” in this work) and copper particles together stimulates the fungal growth and the laccase production during submerged cultivation of T. versicolor. Therefore, it would be a safe and cheap strategy for the commercial production of laccase by filamentous fungi.

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References

  1. Wong AHH, Wilkes J (1988) Progressive changes in cell wall components of Pinus radiata during decay. Int Biodeterior 24(6):481–487. https://doi.org/10.1016/0265-3036(88)90036-X

    Article  CAS  Google Scholar 

  2. Janjušević L, Karaman M, Šibul F et al (2017) The lignicolous fungus Trametes versicolor (L.) Lloyd (1920): a promising natural source of antiradical and AChE inhibitory agents. J Enzyme Inhib Med Chem 32(1):355–362

    PubMed  PubMed Central  Google Scholar 

  3. Schmidt O (2006) Wood and tree fungi. Springer

    Google Scholar 

  4. Taremian A, Faizipour M, Karimi AN, Parsapjoh D (2008) Heterogeneity of the physical properties of Rohtan Noel pine wood (Picea abies) containing compressed wood. Pajouhesh and Sazandegi 20(4):158

    Google Scholar 

  5. Galhaup C, Haltrich D (2001) Enhanced formation of laccase activity by the white-rot fungus Trametes pubescens in the presence of copper. Appl Microbiol Biotechnol 56(1):225–232

    CAS  PubMed  Google Scholar 

  6. Vaithanomsat P, Sangnam A, Boonpratuang T et al (2013) Wood degradation and optimized laccase production by resupinate white-rot fungi in northern Thailand. BioResources 8(4):6342–6360

    Google Scholar 

  7. Chauhan R (2019) Nitrogen sources and trace elements influence Laccase and peroxidase enzymes activity of Grammothele fuligo. Vegetos 32(3):316–323

    Google Scholar 

  8. Sun K, Li S, Si Y, Huang Q (2021) Advances in laccase-triggered anabolism for biotechnology applications. Crit Rev Biotechnol 41(7):969–993

    CAS  PubMed  Google Scholar 

  9. Maciel MJM, Ribeiro HCT (2010) Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: a review. Electron J Biotechnol 13(6):14–15

    Google Scholar 

  10. Riva S (2006) Laccases: blue enzymes for green chemistry. TRENDS Biotechnol 24(5):219–226

    CAS  PubMed  Google Scholar 

  11. Curran LMCLK, Sale KL, Simmons BA (2021) Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts. Biotechnol Adv 54:107809

    Google Scholar 

  12. Pourkhanali K, Khayati G, Mizani F, Raouf F (2021) Isolation, identification and optimization of enhanced production of laccase from Galactomyces geotrichum under solid-state fermentation. Prep Biochem Biotechnol 51(7):659–668

    CAS  PubMed  Google Scholar 

  13. Baldrian P (2006) Fungal laccases–occurrence and properties. FEMS Microbiol Rev 30(2):215–242

    CAS  PubMed  Google Scholar 

  14. Dong JL, Zhang YZ (2004) Purification and characterization of two laccase isoenzymes from a ligninolytic fungus Trametes gallica. Prep Biochem Biotechnol 34(2):179–194

    CAS  PubMed  Google Scholar 

  15. Han SH, Lee JH (2005) An overview of peak-to-average power ratio reduction techniques for multicarrier transmission. IEEE Wirel Commun 12(2):56–65

    CAS  Google Scholar 

  16. D’Souza C, Taghian M, Lamb P (2006) An empirical study on the influence of environmental labels on consumers. Corp Commun Int J 11(2):162–173

    Google Scholar 

  17. Zouari-Mechichi H, Mechichi T, Dhouib A, Sayadi S, Martinez AT, Martinez MJ (2006) Laccase purification and characterization from Trametes trogii isolated in Tunisia: decolorization of textile dyes by the purified enzyme. Enzyme Microb Technol 39(1):141–148

    CAS  Google Scholar 

  18. Haibo Z, Yinglong Z, Feng H, Peiji G, Jiachuan C (2009) Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta. Biotechnol Lett 31(6):837–843

    PubMed  Google Scholar 

  19. Mishra VK, Tripathi BD (2009) Accumulation of chromium and zinc from aqueous solutions using water hyacinth (Eichhornia crassipes). J Hazard Mater 164(2-3):1059–1063

    CAS  PubMed  Google Scholar 

  20. Zapata-Castillo P, Villalonga-Santana M, Tamayo-Cortés J, Rivera-Muñoz G, Solís-Pereira S (2012) Purification and characterization of laccase from Trametes hirsuta Bm-2 and its contribution to dye and effluent decolorization. African J Biotechnol 11(15):3603–3611

    CAS  Google Scholar 

  21. de Araújo DG, Bezerra DS, de Queiroz DD et al (2019) Toxicological and pharmacologic effects of farnesol (C15H26O): a descriptive systematic review. Food Chem Toxicol 129:169–200

    PubMed  Google Scholar 

  22. Lassouane F, Aït-Amar H, Amrani S, Rodriguez-Couto S (2019) A promising laccase immobilization approach for Bisphenol A removal from aqueous solutions. Bioresour Technol 271:360–367

    CAS  PubMed  Google Scholar 

  23. Birhanli E, Yesilada O (2006) Increased production of laccase by pellets of Funalia trogii ATCC 200800 and Trametes versicolor ATCC 200801 in repeated-batch mode. Enzyme Microb Technol 39(6):1286–1293

    CAS  Google Scholar 

  24. Fonseca MI, Shimizu E, Zapata PD, Villalba LL (2009) WITHDRAWN: Laccase-producing ability and the inducing effect of copper on laccase production of white rot fungi native from Misiones (Argentina). Enzym Microb Technol. https://doi.org/10.1016/j.enzmictec.2009.07.003

  25. Jaber SM, Shah UKM, Asa'ari AZM, Ariff AB (2017) Optimization of laccase production by locally isolated Trichoderma muroiana IS1037 using rubber wood dust as substrate. BioResources 12(2):3834–3849

    CAS  Google Scholar 

  26. Větrovský T, Baldrian P, Gabriel J (2013) Extracellular enzymes of the white-rot fungus Fomes fomentarius and purification of 1, 4-β-glucosidase. Appl Biochem Biotechnol 169(1):100–109

    PubMed  Google Scholar 

  27. Backes E, Kato CG, de Oliveira Junior VA et al (2023) Overproduction of Laccase by Trametes versicolor and Pycnoporus sanguineus in farnesol-pineapple waste solid fermentation. Fermentation 9(2):188

    CAS  Google Scholar 

  28. Costa LG, Paes JB, Jesus Junior WC, Brocco VF, Pinho DB (2020) Isolation and identification of fungi with potential for biological stump removal of Eucalyptus spp. J Trop For Sci 32(2):154–160

    Google Scholar 

  29. Safaie N, Alizadeh AA, Saeidi A, Adam G, Rahimian H (2005) Molecular characterization and genetic diversity among iranian populations of Fusarium graminearum, the causal agent of wheat headblight. Iran J Plant Pathol 41(2):171–189

    Google Scholar 

  30. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc Guid Methods Appl 18(1):315–322

    Google Scholar 

  31. Justo A, Hibbett DS (2011) Phylogenetic classification of Trametes (Basidiomycota, Polyporales) based on a five–marker dataset. Taxon 60(6):1567–1583

    Google Scholar 

  32. Giorgio EM, Fonseca MI, Tejerina MR et al (2012) Chips and sawdust substrates application for lignocellulolytic enzymes production by solid state fermentation. Int Res J Biotechnol 3(7):120–127

    Google Scholar 

  33. Stoilova I, Krastanov A, Stanchev V (2010) Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation. Adv Biosci Biotechnol 1:208–215

    CAS  Google Scholar 

  34. Bertrand B, Martínez-Morales F, Tinoco-Valencia R, Rojas S, Acosta-Urdapilleta L, Trejo-Hernández MR (2015) Biochemical and molecular characterization of laccase isoforms produced by the white-rot fungus Trametes versicolor under submerged culture conditions. J Mol Catal B Enzym 122:339–347

    CAS  Google Scholar 

  35. Daâssi D, Zouari-Mechichi H, Frikha F, Rodríguez-Couto S, Nasri M, Mechichi T (2016) Sawdust waste as a low-cost support-substrate for laccases production and adsorbent for azo dyes decolorization. J Environ Heal Sci Eng 14(1):1–12

    Google Scholar 

  36. Niku-Paavola ML, Karhunen E, Kantelinen A, Viikari L, Lundell T, Hatakka A (1990) The effect of culture conditions on the production of lignin modifying enzymes by the white-rot fungus Phlebia radiata. J Biotechnol 13(2-3):211–221

    CAS  Google Scholar 

  37. Sabarathinam S, Jayaraman V, Balasubramanian M, Swaminathan K (2014) Optimization of culture parameters for hyper laccase production by Trichoderma asperellum by Taguchi design experiment using L-18 orthogonal array. Malaya j Biosci 1(4):214–225

    CAS  Google Scholar 

  38. Abd El Aty AA, Wehaidy HR, Mostafa FA (2014) Optimization of inulinase production from low cost substrates using Plackett–Burman and Taguchi methods. Carbohydr Polym 102:261–268

    CAS  PubMed  Google Scholar 

  39. Petlamul W, Prasertsan P (2014) Medium optimization for production of Beauveriabassiana BNBCRC spores from biohydrogen effluent of palm oil mill using Taguchi design. Int J Biosci Biochem Bioinforma 4(2):106

    CAS  Google Scholar 

  40. Azin M, Moravej R, Zareh D (2007) Production of xylanase by Trichoderma longibrachiatum on a mixture of wheat bran and wheat straw: optimization of culture condition by Taguchi method. Enzyme Microb Technol 40(4):801–805

    CAS  Google Scholar 

  41. Revankar MS, Lele SS (2006) Enhanced production of laccase using a new isolate of white rot fungus WR-1. Process Biochem 41(3):581–588

    CAS  Google Scholar 

  42. Saparrat MCN, Guillén F, Arambarri AM, Martínez AT, Martínez MJ (2002) Induction, isolation, and characterization of two laccases from the white rot basidiomycete Coriolopsis rigida. Appl Environ Microbiol 68(4):1534–1540

    CAS  PubMed  PubMed Central  Google Scholar 

  43. Zhu C, Bao G, Huang S (2016) Optimization of laccase production in the white-rot fungus Pleurotus ostreatus (ACCC 52857) induced through yeast extract and copper. Biotechnol Biotechnol Equip 30(2):270–276

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

    Mohammadreza Ensani

  2. Department of Horticulture and Plant Protection, Faculty of Agriculture, Shahrood University of Technology, P. O. Box: 3619995161, Shahrood, Iran

    Shideh Mojerlou

  3. Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Seyedeh Masoumeh Zamani

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  1. Mohammadreza Ensani
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  2. Shideh Mojerlou
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  3. Seyedeh Masoumeh Zamani
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Contributions

Mohammadreza Ensani: investigation, visualization, and writing—original draft. Shideh Mojerlou: conceptualization, supervision, methodology, formal analysis, and writing—review and editing. Seyedeh Masoumeh Zamani: conceptualization, resources, data curation, and writing—review and editing.

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Correspondence to Shideh Mojerlou.

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Ensani, M., Mojerlou, S. & Zamani, S.M. Enhanced laccase activity in Trametes versicolor (L.: Fr.) Pilát by host substrate and copper. Braz J Microbiol 54, 1565–1572 (2023). https://doi.org/10.1007/s42770-023-01096-x

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