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Detection of Sugarcane bacilliform virus in diseased sugarcane plants in Andhra Pradesh, India by serological and molecular methods

  • Bacterial Fungal and Virus Molecular Biology - Research Paper
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Abstract

Sugarcane leaf fleck incited by Sugarcane bacilliform virus is emerging as a major disease and affecting exchange of sugarcane germplasm and cultivation worldwide. Roving surveys conducted in 162 fields belonging to 81 villages spread over 14 sugarcane growing districts of Andhra Pradesh during 2021–2022 revealed 8 to 44% incidence of the disease. Mean maximum fleck disease incidence was reported in Anakapalli district (33.00%) followed by Srikakulam district (22.66%), whereas least incidence was observed in Alluri Sitharamaraju district (9.33%). The early and sensitive detection of pathogens is vital and necessary to reduce the danger of introducing new diseases or pathogen strains into sugarcane growing regions. Both serological and molecular methods were used in proposed investigation to identify the virus at the protein and nucleic acid levels. DAS-ELISA results were positive for 50 suspected SCBV infected sugarcane leaf samples out of 81, with mean absorbance (A405) values ranging from 0.50 to 2.20. Further PCR assays were performed using SCBV-specific primers targeting RT/RNase H coding region which is frequently employed as a taxonomic marker for species delineation in Badnaviruses. Out of 81 symptomatic samples collected, 61 samples gave positive results, and no amplification was observed in healthy control and negative control. Results made it evident that PCR was more sensitive than DAS-ELISA. Low virus concentration or variation in virus strains may be the reason for the low detection rate in DAS-ELISA in the current study. Extensive roving surveys conducted for the incidence of leaf fleck disease for the first time in the state of Andhra Pradesh revealed severe occurrence of leaf fleck disease under field conditions.

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Acknowledgements

The work has been undertaken as part of the of Ph.D research program in Agricultural college, Bapatla, ANGRAU. I am thankful to the Advisory committee for their support during Ph. D program.

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The research work was supported by the Institute grants of ANGRAU, and no separate funding has been received to the research work reported in the paper.

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Authors and Affiliations

  1. Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G Ranga Agricultural University, Guntur, Andhra Pradesh, India

    G. Vamsi Krishna & V. Manoj Kumar

  2. Department of Plant Pathology, Regional Agricultural Research Station, Lam, Guntur, Andhra Pradesh, India

    P. Kishore Varma

  3. Department of Entomology, DAATT Centre, Amalapuram, Acharya N.G Ranga Agricultural University, Guntur, Andhra Pradesh, India

    B. Bhavani

  4. Department of Crop Physiology, Agricultural College, Bapatla, Acharya N.G Ranga Agricultural University, Guntur, Andhra Pradesh, India

    G. Vijaya Kumar

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  1. G. Vamsi Krishna
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  4. B. Bhavani
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Contributions

PKV designed the study, GVK carried out the work, and wrote the manuscript. BB, VMK, and VK analyzed the data. All authors read and approved the submitted manuscript.

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Correspondence to G. Vamsi Krishna.

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Krishna, G.V., Kumar, V.M., Varma, P.K. et al. Detection of Sugarcane bacilliform virus in diseased sugarcane plants in Andhra Pradesh, India by serological and molecular methods. Braz J Microbiol 54, 1399–1409 (2023). https://doi.org/10.1007/s42770-023-01094-z

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