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Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

N-Acetyl-glucosaminidases (GlcNAcases) are exoenzymes found in a wide range of living organisms, which have gained great attention in the treatment of disorders related to diabetes, Alzheimer’s, Tay-Sachs’, and Sandhoff’s diseases; the control of phytopathogens; and the synthesis of bioactive GlcNAc-containing products. Aiming at future industrial applications, in this study, GlcNAcase production by marine Aeromonas caviae CHZ306 was enhanced first in shake flasks in terms of medium composition and then in bench-scale stirred-tank bioreactor in terms of physicochemical conditions. Stoichiometric balance between the bioavailability of carbon and nitrogen in the formulated culture medium, as well as the use of additional carbon and nitrogen sources, played a central role in improving the bioprocess, considerably increasing the enzyme productivity. The optimal cultivation medium was composed of colloidal α-chitin, corn steep liquor, peptone A, and mineral salts, in a 5.2 C:N ratio. Optimization of pH, temperature, colloidal α-chitin concentration, and kLa conditions further increased GlcNAcase productivity. Under optimized conditions in bioreactor (i.e., 34 °C, pH 8 and kLa 55.2 h−1), GlcNAcase activity achieved 173.4 U.L−1 after 12 h of cultivation, and productivity no less than 14.45 U.L−1.h−1 corresponding to a 370-fold enhancement compared to basal conditions.

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Funding

This work was supported by the Sao Paulo Research Foundation (FAPESP), Brazil, [grant numbers 2012/16824–0 and 2013/18773–6].

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Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brasil

    Flávio Augusto Cardozo

  2. Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brasil

    Valker Feitosa, Carlos Miguel Nóbrega Mendonça, Francisco Vitor Santos da Silva, Attilio Converti, Ricardo Pinheiro de Souza Oliveira & Adalberto Pessoa Jr

  3. Departamento de Medicina e Enfermagem, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa, Brasil

    Valker Feitosa

  4. CICECO – Instituto de Materiais de Aveiro, Departamento de Química, Universidade de Aveiro, Aveiro, Portugal

    Carlos Miguel Nóbrega Mendonça

  5. Dipartimento di Ingegneria Civile, Chimica e Ambientale, Università di Genova, Genova, Italia

    Attilio Converti

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Cardozo, F.A., Feitosa, V., Mendonça, C.M.N. et al. Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor. Braz J Microbiol 54, 1533–1545 (2023). https://doi.org/10.1007/s42770-023-01088-x

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