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Comparative genomic and phenotypic analyses of pathogenic fungi Neoscytalidium dimidiatum and Bipolaris papendorfii isolated from human skin scraping

  • Bacterial and Fungal Pathogenesis - Research Paper
  • Published:
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Abstract  

Neoscytalidium dimidiatum and Bipolaris species are fungal plant pathogens that have been reported to cause human diseases. Recently, we have isolated numerous N. dimidiatum and Bipolaris species from the skin scrapings and nails of different patients. In this work, we have sequenced the genome of one strain of N. dimidiatum. The sequenced genome was compared to that of a previously reported Bipolaris papendorfii genome for a better understanding of their complex lifestyle and broad host-range pathogenicity. Both N. dimidiatum UM 880 (~ 43 Mb) and B. papendorfii UM 226 (~ 33 Mb) genomes include 11,015–12,320 putative coding DNA sequences, of which 0.51–2.49% are predicted transposable elements. Analysis of secondary metabolism gene clusters revealed several genes involved in melanin biosynthesis and iron uptake. The arsenal of CAZymes related to plants pathogenicity is comparable between the species, including genes involved in hemicellulose and pectin decomposition. Several important gene encoding keratinolytic peptidases were identified in N. dimidiatum and B. papendorfii, reflecting their potential pathogenic role in causing skin and nail infections. In this study, additional information on the metabolic features of these two species, such as nutritional profiling, pH tolerance, and osmotolerant, are revealed. The genomic characterization of N. dimidiatum and B. papendorfii provides the basis for the future functional studies to gain further insights as to what makes these fungi persist in plants and why they are pathogenic to humans.

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Data availability

The data that support the results of this study are accessible via GenBank assembly accession: GCA_900092665.1. The genome of B. papendorfii UM 226 is accessible via GenBank assembly accession: GCA_000817285.1.

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Funding

This work was supported by High Impact Research MOE Grant (UM.C/625/1/HIR/MOHE/MED/31, account no: H-20001–00-E000070) from the Ministry of Education Malaysia and partly by Putra Grant—Putra Graduate Initiative (IPS) from Universiti Putra Malaysia (GP-IPS/2018/9613300).

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Chee Sian Kuan, Kee Peng Ng, Su Mei Yew, Rukumani Devi Velayuthan & Shiang Ling Na

  2. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Hadiza Umar Meleh, Siti Norbaya Masri & Leslie Thian Lung Than

  3. Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Heng Fong Seow

  4. Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Kang Nien How

  5. School of Biosciences, Taylor’s University Lakeside Campus, Subang Jaya, 47500, Selangor, Malaysia

    Siok Koon Yeo & Jap Meng Jee

  6. Codon Genomics Sdn Bhd, 43200 Seri Kembangan, Selangor, Malaysia

    Yung-Chie Tan, Wai-Yan Yee & Chee-Choong Hoh

  7. Department of Pathology and Pharmacology, School of Medicine, International Medical University, 57000, Kuala Lumpur, Malaysia

    Shu Yih Chew

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  1. Chee Sian Kuan
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Contributions

Conceptualization: CSK, KPN, and LTLT; data curation: CSK, SMY, YCT, WYY, CCH, and SYC; funding acquisition: KPN and LTLT; investigation: CSK, SMY, HUM, SKY, JMJ, and RDV; methodology: CSK, SMY, JMJ, RDV, SLN, and SYC; project administration: CSK; resources: KPN and LTLT; software: YCT, WYY, and CCH; supervision: KPN and LTLT; validation: CSK; visualization: CSK; writing—original draft: CSK and SKY; writing—review and editing: KPN, SMY, HUM, HFS, KNH, SNM, SYC, and LTLT.

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Correspondence to Leslie Thian Lung Than.

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Kuan, C.S., Ng, K.P., Yew, S.M. et al. Comparative genomic and phenotypic analyses of pathogenic fungi Neoscytalidium dimidiatum and Bipolaris papendorfii isolated from human skin scraping. Braz J Microbiol 54, 1351–1372 (2023). https://doi.org/10.1007/s42770-023-01032-z

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  • DOI: https://doi.org/10.1007/s42770-023-01032-z

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