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In vitro nematicidal and acaricidal effect of biosurfactants produced by Bacillus against the root-knot nematode Nacobbus aberrans and the dust mite Tyrophagus putrescentiae

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Abstract

In the present study, the nematicidal and acaricidal activity of three biosurfactants (BS) produced by strains of the Bacillus genus was evaluated. The BS produced by the Bacillus ROSS2 strain presented a mortality of 39.29% in juveniles (J2) of Nacobbus aberrans at a concentration of 30 mg/mL, this same strain is the one that presented the highest mortality in Tyrophagus putrescentiae, which was 57.97% at a concentration of 39 mg/mL. The BS were qualitatively identified by thin layer chromatography and are lipid in nature based on the retention factor (Rf). While the GC-MS analysis identified two main compounds that are 4,7-Methano-1H-indene-2,6-dicarboxylic acid, 3a,4,7,7a-tetrahydro-1, and Methyl 4-(pyrrol-1-yl)-1,2,5-oxadiazole-3-carboxylate1, which is the polar part indicated by the presence of dicarboxylic acid and carboxylate groups; while the non-polar portion can be interpreted as a hydrocarbon chain of variable length. Based on the present results, BS can be an alternative for the biocontrol of the root-knot nematode N. aberrans and the mite T. putrescentiae.

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Acknowledgements

The authors thank M.C. Susan Yaracet Páez-León and IBT. Angelita Morales-Morales for assisting with the research. This study was partially financed by the Fiscal Resources Project of INIFAP with the number: 139335341.

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Authors and Affiliations

  1. Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos Cuernavaca, Cuernavaca, Morelos, C.P, 62209, México

    Jaime Adriel Gómez-Gutiérrez, Juan Manuel Caspeta-Mandujano & Alejandro García-Flores

  2. División Agroalimentaria, Universidad Tecnológica de la Selva, C.P, 29950, Ocosingo, Mexico

    Arnoldo Wong-Villarreal & Julio Cruz-Arévalo

  3. National Center for Disciplinary Research in Animal Health and Safety (INIFAP), Km 11 Federal Road Cuernavaca-Cuautla, 62550, Jiutepec, MR, Mexico

    Liliana Aguilar-Marcelino & Patricia Vargas-Uriostegui

  4. Laboratorio de edafología y ambiente. Facultad de ciencias, Universidad Autónoma del estado de Mexico, Campus El Cerrillo, Carretera Toluca-Ixtlahuaca Km 15.5, Piedras Blancas, C.P, 50200, Toluca de Lerdo, México

    Gustavo Yañez-Ocampo

  5. Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Recursos del Mar, Unidad Mérida, Mérida, Yucatán, México

    Emanuel Hernández-Nuñéz

  6. Programa de Fitopatología, Colegio de Postgraduados-Campus Montecillo, km. 36.5 Carretera México-Texcoco, 56230, Texcoco, Estado de México, México

    Olga Gomez-Rodríguez

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  1. Jaime Adriel Gómez-Gutiérrez
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Gómez-Gutiérrez, J.A., Wong-Villarreal, A., Aguilar-Marcelino, L. et al. In vitro nematicidal and acaricidal effect of biosurfactants produced by Bacillus against the root-knot nematode Nacobbus aberrans and the dust mite Tyrophagus putrescentiae. Braz J Microbiol 54, 1127–1136 (2023). https://doi.org/10.1007/s42770-023-00981-9

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