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Isolation and identification of the endophytic fungus J2-3 and its disease-preventive and growth-promoting effects on cucumber

  • Soil and Agricultural Microbiology - Research Paper
  • Published:
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Abstract

There are many problems that result from the use of a large number of chemical pesticides to control plant diseases, including pathogenic bacteria resistance, environmental contamination, and human health effects. Recently, endophytic fungi have become a significant source of bioactive fungicide products and an invaluable resource for excavating microbial pesticides. In this study, endophytic fungi with biocontrol potential were isolated and screened from Mikania micrantha leaves, stems, and roots. Fifty endophytic fungi were isolated and their antagonistic activity was studied in vitro using the confrontation culture method. The J2-3 strains from stems exhibit broad-spectrum and high activity. The strain’s biological characteristics were determined by various culture conditions, and it was identified as Fusarium proliferatum by both morphological and ITS sequence analysis. Biological characteristics of the J2-3 strain were also tested. The optimum temperature for mycelium growth and sporulation was 25 °C and 30 °C, respectively. For mycelium growth, starch was the optimum carbon source, and peptone was the optimum nitrogen source for sucrose, mycelium growth, and sporulation. Mycelium growth was killed by a temperature of 60 °C, and sporulation was killed by a temperature of 55 °C. The light aided mycelium growth, and the light alternated between light and dark cycles for sporulation. Further, pot experiments were conducted to determine the antagonistic and viable effects of highly antagonistic strains on cucumber. The spore suspension’s final control efficacy on cucumber wilt disease was up to 62.79% and it also promoted cucumber growth significantly. The results show that the entophytic fungus J2-3 from M. micrantha can protect cucumbers from wilt disease and promote growth.

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References

  1. Ristaino JB, Anderson PK, Bebber DP et al (2021) The persistent threat of emerging plant disease pandemics to global food security. PNAS 118(23):e2022239118

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Delgado L, Schuster M, Torero M (2017) “The reality of food losses: a new measurement methodology” (IFPRI Discussion Paper 1686, International Food Policy Research Institute, Washington, DC). http://ebrary.ifpri.org/cdm/ref/collection/p15738coll2/id/131530

  3. Yang SH, Wang D, Chen C, Xu CL, Xie H (2020) Evaluation of Stratiolaelaps scimitus (Acari: Laelapidae) for controlling the root-knot nematode, Meloidogyne incognita (Tylenchida: Heteroderidae). Sci Rep 10:5645

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Bernauer OM, Gaines-Day HR, Steffan SA (2015) Colonies of bumble bees (Bombus impatiens) produce fewer workers, less bee biomass, and have smaller mother queens following fungicide exposure. Insects 6:478–488

    Article  PubMed  PubMed Central  Google Scholar 

  5. Gupta PK (2018) Toxicity of fungicides. In: Gupta RC (ed) Veterinary toxicology: basic and clinical principles. Academic Press 845:569–580

  6. Vielba-Fernández A, Polonio Á, Ruiz-Jiménez L, De Vicente A, Pérez-García A, Fernández-Ortuño D (2020) Fungicide resistance in powdery mildew fungi. Microorganisms 8(9):1431

    Article  PubMed  PubMed Central  Google Scholar 

  7. Kubheka SF, Tesfay SZ, Mditshwa A, Magwaza LS (2020) Evaluating the efficacy of edible coatings incorporated with Moringa leaf extract on postharvest of ‘Maluma’avocado fruit quality and its biofungicidal effect. HortScience 55:410–415

    Article  CAS  Google Scholar 

  8. Lu H, Wei T, Lou H, Shu X, Chen Q (2021) A critical review on communication mechanism within plant-endophytic fungi interactions to cope with biotic and abiotic stresses. J Fungi 7(9):719

    Article  CAS  Google Scholar 

  9. Manrique V, Diaz R, Cuda JP, Overholt WA (2011) Suitability of a new plant invader as a target for biological control in Florida. Invasive Plant Sci Manag 4:1–10

    Article  Google Scholar 

  10. Shen J, Wang Z, Su Y, Wang T (2021) Associations between population epigenetic differentiation and environmental factors in the exotic weed mile-a-minute (Mikania micrantha). Weed Sci 69:307–332

    Article  Google Scholar 

  11. Day MD, Clements DR, Gile C, Senaratne WKAD, Shen S, Weston LA et al (2016) Biology and impacts of pacific islands invasive species. 13. Mikania micrantha Kunth (Asteraceae). Pac Sci 70:257–285

    Article  Google Scholar 

  12. Jiang YZ, Wang YT, Zheng YP, Cai ML, Peng CL, Li WH (2021) Physiological and transcriptomic responses of Mikania micrantha stem to shading yield novel insights into its invasiveness. Biol Invasions 23(9):2927–2943

    Article  Google Scholar 

  13. Zhang LY, Ye WH, Cao HL, Feng HL (2004) Mikania micrantha H. B. K. in China - an overview. Weed Res 44(1): 42–49

  14. Deori C, Dutta G, Das S, Phukan D (2016) Analgesic activity of ethanolic extract of leaves of Mikania micrantha on experimental animal models. Pharma Sci Monit 7(3):168–173

    CAS  Google Scholar 

  15. Rios VE, Leon A, Chavez MI, Torres Y, Ramirez-Apan MT, Toscano RA, Bravo-Monzon AE, Espinosa-Garcia FJ, Delgado G (2014) Sesquiterpene lactones from Mikania micrantha and Mikania cordifolia and their cytotoxic and antiinflammatory evaluation. Fitoterapia 94:155–163

    Article  PubMed  Google Scholar 

  16. Jyothilakshmi M, Helen LR, Jyothis M, Sankunni LM (2015) Trypsin inhibiting activity of methanolic extract of aerial parts of mikania micrantha. Int J Pharma Sci Res 6(2):259–261

    CAS  Google Scholar 

  17. Khatun R, Roy S, Rahman MAA (2017) In vitro comparative evaluation of anti-inflammatory and thrombolytic activity of three Mikania species available in Bangladesh. J Pharmacogn Phytochem 6(5):1007–1011

    CAS  Google Scholar 

  18. Li Y, Li J, Li Y, Wang XX, Cao AC (2013) Antimicrobial constituents of the leaves of Mikania micrantha H B K. PLoS One 8(10):e76725

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Tamang D, Phukan BC, Dutta P, Devi U, Malik V (2016) Phytochemical and antimicrobial screening of some weeds of asteraceae family and widely known medicinal herb Paederia foetida L. Int J Pharm Sci Rev Res 40(2):103–108

    CAS  Google Scholar 

  20. Harahap NI, Nainggolan M, Harahap U (2018) Formulation and evaluation of herbal antibacterial gel containing ethanolic extract of mikania micrantha kunth leaves. Asian J Pharm Clin Res 11(3):429

    Article  Google Scholar 

  21. But PPH, He ZD, Ma SC, Chan YM, Shaw PC, Ye WC, Jiang RW (2009) Antiviral constituents against respiratory viruses from Mikania micrantha. J Nat Prod 72(5):925–928

    Article  CAS  PubMed  Google Scholar 

  22. Gansau JA, Matawali A, Chin LP, Eng HS, Boon LH (2016) In-vitro evaluation of anti-kinase, anti-phosphatase and cytotoxic activities of mikania micrantha h.b.k. (asteraceae) from malaysia. J Chem Pharm Sci 9(2):696–701

  23. Sharma HK, Mishra S, Kumar A (2011) Evaluation of in vitro antioxidant activity of the methanolic extract of the leaves of Mikania micrantha Kunth. Asian J Chem 23(10):4525–4527

    CAS  Google Scholar 

  24. Lallianchhunga MC, Ali MA, Lalchhandama C, Lalmuanthanga C, Devi L (2016) Antioxidant activity of methanolic extract of Mikania micrantha leaves. World J Pharm Res 5(4):879–886

    CAS  Google Scholar 

  25. Ishak AH, Shafie NH, Esa NM, Bahari H, Ismail A (2018) From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha. Int J Agric Biol 20(3):561–568

    Article  CAS  Google Scholar 

  26. Xu Q, Xie H, Xiao H, Lin L, Wei X (2013) Two new ent-kaurene diterpene glucosides from the roots of Mikania micrantha. Phytochem Lett 6(3):425–428

    Article  CAS  Google Scholar 

  27. Dong LM, Jia XC, Luo QW, Peng YM, Zhang Q, Luo B, Tan JW (2017) Four new ent-kaurene diterpene glucosides from Mikania micrantha. Phytochem Lett 20:155–159

    Article  CAS  Google Scholar 

  28. Laurella LC, Cerny N, Bivona AE, Andres Sanchez A, Giberti G, Malchiodi EL, Martino VS, Catalan CA, Alonso MR, Cazorla SI, Sülsen VP (2017) Assessment of sesquiterpene lactones isolated from Mikania plants species for their potential efficacy against Trypanosoma cruzi and Leishmania sp. PLoS Neglected Trop Dis 11(9):e0005929

    Article  Google Scholar 

  29. Zhang Y, Zeng YM, Xu YK, Wu SQ, Shen L, Tian HY, Sheng Y (2020) New cadinane sesquiterpenoids from Mikania micrantha. Nat Prod Res 34(19):2729–2736

    Article  CAS  PubMed  Google Scholar 

  30. Xu Q, Xie H, Xiao H, Wei X (2013) Phenolic constituents from the roots of mikania micrantha and their allelopathic effects. J Agric Food Chem 61(30):7309–7314

    Article  CAS  PubMed  Google Scholar 

  31. Han D, Wang L, Luo Y (2018) Isolation, identification, and the growth promoting effects of two antagonistic actinomycete strains from the rhizosphere of Mikania micrantha Kunth. Microbiol Res 208:1–11

    Article  CAS  PubMed  Google Scholar 

  32. Linnakoski R, Puhakka-tarvainen H, Pappinen A (2012) Endophytic fungi isolated from Khaya anthotheca in Ghana. Fungal Ecol 5(3):298–308

    Article  Google Scholar 

  33. Sun G, Yao T, Feng C, Chen L, Li J, Wang L (2017) Identification and biocontrol potential of antagonistic bacteria strains against sclerotinia sclerotiorum and their growth-promoting effects on brassica napus. Biol Control 104:35–43

    Article  CAS  Google Scholar 

  34. Manganyi MC, Regnier T, Kumar A, Bezuidenhout CC, Ateba CN (2018) Phylogenetic analysis and diversity of novel endophytic fungi isolated from medicinal plant Sceletium tortuosum. Phytochem Lett 27:36–43

    Article  CAS  Google Scholar 

  35. Tamura K, Stecher G, Peterson D et al (2013) MEGA 6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Mellon JE, Dowd MK, Beltz SB (2013) Effects of temperature and medium composition on inhibitory activities of gossypol-related compounds against aflatoxigenic fungi[J]. J Appl Microbiol 115(1):179–186

    Article  CAS  PubMed  Google Scholar 

  37. Nowakowska M, Wrzesi ´nska M, Kami´ nski P, Szczechura W, Lichocka M, Tartanus M, Kozik EU, Nowick M (2019) Alternaria brassicicola–Brassicaceae pathosystem: insights into the infection process and resistance mechanisms under optimized artificial bio-assay. Eur J Plant Pathol 153: 131–151

  38. Dong J, Xu J, Xu X, Xu Q, Chen X (2019) Inheritance and quantitative trait locus mapping of Fusarium Wilt resistance in cucumber. Front Plant Sci 10:01425

    Article  Google Scholar 

  39. He J, Shi Y, Zhao J, Yu Z (2019) Strip rotary tillage with a two-year subsoiling interval enhances root growth and yield in wheat. Sci Rep 9(1):11678

    Article  PubMed  PubMed Central  Google Scholar 

  40. Xu Y, Yuan Y, Du N, Wang Y, Shu S, Sun J, Guo S (2018) Proteomic analysis of heat stress resistance of cucumber leaves when grafted onto Momordica rootstock. Hortic Res 5(1):1–18

    Article  PubMed  PubMed Central  Google Scholar 

  41. Gerlach W, Nirenberg H (1983) The genus Fusarium: a pictorial atlas. Mycol Soc Am 75(6):1110

    Article  Google Scholar 

  42. Leslie JF, Summerell BA (2008) The Fusarium laboratory manual. John Wiley & Sons

    Google Scholar 

  43. Bogdal D, Prociak A (2007) Microwave-enhanced polymer chemistry and technology. Blackwell Publishing Professional, Ames, IA, USA, pp 3–31

    Google Scholar 

  44. Balajee SA, Borman AM, Brandt ME (2009) Sequence-based identification of Aspergillus, Fusarium, and Mucorales species in the clinical mycology laboratory: where are we and where should we go from here? J Clin Microbiol 47(4):877–884

    Article  CAS  PubMed  Google Scholar 

  45. Cheng Z, Tang W, Su Z (2008) Identification of mangrove endophytic fungus 1403 (Fusarium proliferatum) based on morphological and molecular evidence. J For Res (Harbin, China) 19(3):219–224

    Google Scholar 

  46. Singh A, Kumar J, Sharma VK, Singh DK, Kumari P, Nishad JH et al (2021) Phytochemical analysis and antimicrobial activity of an endophytic Fusarium proliferatum (ACQR8), isolated from a folk medicinal plant Cissus quadrangularis L. S Afr J Bot 140:87–94

    Article  CAS  Google Scholar 

  47. Jiang CX, Li J, Zhang JM, Jin XJ, Yu B, Fang J, Wu QX (2019) Isolation, identification, and activity evaluation of chemical constituents from the soil fungus Fusarium avenaceum SF-1502 and endophytic fungus Fusarium proliferatum AF-04. J Agric Food Chem 67:1839–1846

    Article  CAS  PubMed  Google Scholar 

  48. Guo Z, Zhang X, Wu J, Yu J, Xu M, Chen D et al (2020) In vitro inhibitory effect of the bacterium Serratia marcescens on Fusarium proliferatum growth and fumonisins production. Biol Control 143:104188

    Article  CAS  Google Scholar 

  49. Reyes Gaige A, Todd T, Stack JP (2020) Interspecific competition for colonization of maize plants between Fusarium proliferatum and Fusarium verticillioides. Plant Dis 104(8):2102–2110

    Article  PubMed  Google Scholar 

  50. Mondani L, Chiusa G, Pietri A, Battilani P (2020) Monitoring the incidence of dry rot caused by Fusarium proliferatum in garlic at harvest and during storage. Postharvest Biol Technol 173:111407

    Article  Google Scholar 

  51. Li T, Wu Y, Wang Y, Gao H, Gupta VK, Duan X et al (2019) Secretome profiling reveals virulence-associated proteins of Fusarium proliferatum during interaction with banana fruit. Biomolecules 9(6):246

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Bhatt P, Zhang W, Lin Z, Pang S, Huang Y, Chen S (2020) Biodegradation of allethrin by a novel fungus Fusarium proliferatum strain CF2, isolated from contaminated soils. Microorganisms 8(4):593

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding

This study was supported by the Hainan Province Science and Technology special fund (ZDYF2022XDNY274) and Guangzhou Science and Technology Project (202002020044).

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Authors and Affiliations

  1. School of Plant Protection, Hainan University, Haikou, 570228, Hainan, China

    Jiantao Fu, Yuejie Wu, Xiangnan Yan, Lanying Wang, Shujing Zhang & Yanping Luo

  2. Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou, 510316, Guangdong, China

    Jiantao Fu

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  1. Jiantao Fu
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Contributions

Jiantao Fu wrote the manuscript for the article; Yanping Luo designed this work and revised the manuscript. Yuejie Wu and Xiangnan Yan conducted the laboratory assays. Lanying Wang and Shujing Zhang conducted the pot experiment and analyzed the data regarding the growth-promoting effect on cucumber. All authors read and approved the final version of the manuscript.

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Correspondence to Yanping Luo.

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Fu, J., Wu, Y., Yan, X. et al. Isolation and identification of the endophytic fungus J2-3 and its disease-preventive and growth-promoting effects on cucumber. Braz J Microbiol 54, 1115–1125 (2023). https://doi.org/10.1007/s42770-023-00979-3

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