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Prosthetic joint infection caused by an imipenem-resistant Mycobacterium senegalense

  • Clinical Microbiology - Short Communication
  • Published:
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Abstract

Periprosthetic joint infection (PJI) remains one of the most common complications of total knee arthroplasty. Although mainly caused by Staphylococcus aureus and other Gram-positive microorganisms, occasionally, commensal or environmental bacteria are reported as causative agents of these infections. The present work aimed to report a case of PJI caused by an imipenem-resistant Mycobacterium senegalense strain. A bacterial strain isolated from the culture of intraoperative samples was observed by optical microscopy after Gram and Ziehl–Neelsen staining. The species identification was performed by mass spectrometry analysis and partial sequencing of the heat shock protein 65 (hsp65) gene. The antimicrobial profile of the clinical isolate was determined according to the Clinical and Laboratory Standards Institute. Mass spectrometry and gene sequencing analysis identified the bacterial isolate as Mycobacterium fortuitum complex and M. senegalense, respectively. The isolated was found exhibiting an imipenem-resistant profile. The accurate and timely identification, as well as investigation of the antimicrobial susceptibility profile, of fast-growing nontuberculous mycobacteria species are crucial for establishing the prompt and correct treatment of the infection, particularly in cases of patients at greater risk for opportunistic and severe infections.

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Fig. 1

Data Availability

The hsp65 gene sequence of the clinical isolate M. senegalense HP 5643050 was deposited into the GenBank® (NCBI, USA) database under accession number ON000834 and are available at the following URL: https://www.ncbi.nlm.nih.gov/nuccore/ON000834.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Rio de Janeiro, Rio de Janeiro, Brazil

    Louisy Sanches dos Santos & Lincoln de Oliveira Sant’Anna

  2. Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Rafael Theodoro

  3. Operational Technical Nucleus, Microbiology, Hermes Pardini Institute, Vespasiano, Minas Gerais, Brazil

    Nadir Nayara Carvalho dos Santos, Luisa Ferreira Seabra & Max Roberto Batista Araújo

  4. Operational Technical Nucleus, Molecular Biology, Hermes Pardini Institute, Vespasiano, Minas Gerais, Brazil

    Bruna Karoline Lopes Armond

  5. Operational Technical Nucleus, Research and Development, Hermes Pardini Institute, Minas Gerais, Vespasiano, Brazil

    Luige Biciati Alvim

Authors
  1. Louisy Sanches dos Santos
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  2. Lincoln de Oliveira Sant’Anna
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  3. Rafael Theodoro
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  4. Nadir Nayara Carvalho dos Santos
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  5. Bruna Karoline Lopes Armond
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  6. Luisa Ferreira Seabra
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  7. Luige Biciati Alvim
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  8. Max Roberto Batista Araújo
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Contributions

Conceptualization: Louisy Sanches dos Santos and Max Roberto Batista Araújo. Methodology: Lincoln de Oliveira Sant’Anna, Rafael Theodoro, Luige Biciati Alvim, and Max Roberto Batista Araújo. Formal analysis: Louisy Sanches dos Santos, Lincoln de Oliveira Sant’Anna, and Max Roberto Batista Araújo. Investigation: Luisa Ferreira Seabra, Nadir Nayara Carvalho dos Santos, Rafael Theodoro, and Luige Biciati Alvim. Writing—original draft preparation: Louisy Sanches dos Santos and Lincoln de Oliveira Sant’Anna. Writing—review and editing: Louisy Sanches dos Santos, Lincoln de Oliveira Sant’Anna, Nadir Nayara Carvalho dos Santos, Rafael Theodoro, Bruna Karoline Lopes Armond, Luisa Ferreira Seabra, Luige Biciati Alvim, and Max Roberto Batista Araújo. Resources: Bruna Karoline Lopes Armond, Luige Biciati Alvim, and Max Roberto Batista Araújo. Visualization: Louisy Sanches dos Santos. Supervision: Louisy Sanches dos Santos. Project administration: Louisy Sanches dos Santos and Max Roberto Batista Araújo.

Corresponding author

Correspondence to Louisy Sanches dos Santos.

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Ethical approval

The study was a retrospective analysis of laboratory data. No ethical approval was applied.

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The authors declare no competing interests.

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Responsible Editor: Fernando R. Pavan

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dos Santos, L.S., de Oliveira Sant’Anna, L., Theodoro, R. et al. Prosthetic joint infection caused by an imipenem-resistant Mycobacterium senegalense. Braz J Microbiol 54, 929–934 (2023). https://doi.org/10.1007/s42770-023-00960-0

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  • DOI: https://doi.org/10.1007/s42770-023-00960-0

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