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Corynebacterium guaraldiae sp. nov.: a new species of Corynebacterium from human infections

  • Clinical Microbiology - Research Paper
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Abstract

Non-diphtheria Corynebacterium species (NDC) belonging to the human skin and mucosa microbiota are frequently neglected as contaminants. However, reports of human infections by Corynebacterium spp. have increased considerably in recent years. In this study, a group of six NDC isolates of urine (n = 5) and sebaceous cyst (n = 1) from two South American countries were identified at genus level or misidentified based on API® Coryne and genetic/molecular analyses. The 16S rRNA (99.09–99.56%) and rpoB (96.18–97.14%) gene sequence similarities of the isolates were higher when compared with Corynebacterium aurimucosum DSM 44532 T. Multilocus sequence analysis (MLSA) indicated that these six NDC isolates compose a distinctive phylogenetic clade. Genome-based taxonomic analysis with the whole-genome sequences was able to separate these six isolates from other known Corynebacterium type strains. Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA–DNA hybridization (dDDH) values between closely related type strains and the six isolates were considerably lower than the currently recommended threshold values for species circumscription. Phylogenetic and genomic taxonomy analyses indicated these microorganisms as a novel Corynebacterium species, for which we formally propose the name Corynebacterium guaraldiae sp. nov. with isolate 13T (= CBAS 827T = CCBH 35012T) as type strain.

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Acknowledgements

We offer our deepest thanks to Raphael Hirata Jr. (In Memoriam), our eternal teacher and source of inspiration and to Rafael Sanches for his availability in advising on editing the image of this study. In addition, we thank CNPq for the research fellowship granted to LGCP.

Funding

This work was supported by Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES) # 88882.450376/2019–01, and # 88882.315426/2019–01, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) # E-26/202.087/2020, # E-26/210.889/2019, and # E-26/211.629/2015, Fundação Oswaldo Cruz (INOVA/FIOCRUZ) # 7481297331, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) # 309948/2018–5, and Secretaria de Ciencia y Técnica de la Universidad de Buenos Aires (UBACyT) # 20020170100109 BA.

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Authors and Affiliations

  1. Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, Brazil

    Lincoln de Oliveira Sant’Anna, Louisy Sanches dos Santos, Juliana Nunes Ramos & Ana Luíza Mattos-Guaraldi

  2. Hermes Pardini Institute, Vespasiano, Brazil

    Max Roberto Batista Araújo

  3. Department of Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil

    Danilo Jobim Passos Gil da Rocha & Luis Gustavo Carvalho Pacheco

  4. Interdisciplinary Laboratory of Medical Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil

    Juliana Nunes Ramos & Verônica Viana Vieira

  5. Army Chemical and Pharmaceutical Laboratory, Ministry of Defense, Brasília, Brazil

    Paulo Victor Pereira Baio

  6. Richet Laboratory, Rio de Janeiro, Brazil

    Pedro Fernandez Del Peloso & Cassiana da Costa Ferreira Leite

  7. Professur Fuer Mikrobiologie, Friedrich-Alexander-Universität Erlagen-Nürnberg, Erlangen, Germany

    Renata Stavrakakis Peixoto & Andreas Burkovski

  8. Faculty of Pharmacy and Biochemistry and Bacteriology, Department of Clinical Biochemistry, University of Buenos Aires, Autonomous City of Buenos Aires, Buenos Aires, Argentina

    Marisa Almuzara, Carlos Vay & Claudia Barberis

  9. Faculty of Health and Life Sciences, Northumbria University, Newcastle Upon Tyne, UK

    Vartul Sangal

  10. Virus Bioinformatics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Ilhéus, Brazil

    Eric Roberto Guimarães Rocha Aguiar

Authors
  1. Lincoln de Oliveira Sant’Anna
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  2. Louisy Sanches dos Santos
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  3. Max Roberto Batista Araújo
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Contributions

LOS: Data curation; Formal analysis; Writing-original draft; Writing-review and editing; Investigation; Visualization. LSS: Data curation; Formal analysis; Writing-review and editing. MRBA: Writing-original draft; Investigation; Visualization. DJPR: Data curation; Formal analysis; Methodology. JNR: Data curation; Formal analysis; Writing-review and editing. PVPB: Data curation; Formal analysis; Writing-review and editing. PFDP: Writing-review and editing; Investigation; Resources. CCFL: Writing-review and editing; Investigation. RPS: Writing-original draft; Investigation. MA: Writing-review and editing; Investigation; Validation. CV: Data curation; Formal analysis; Writing-review and editing; Investigation; Methodology; Resources. CB: Data curation; Formal analysis; Writing-review and editing; Investigation; Methodology. VS: Data curation; Formal analysis; Writing-review and editing; Resources; Visualization; Validation. AB: Data curation; Formal analysis; Writing-review and editing; Resources; Visualization; Validation; Funding acquisition. ERGRA: Data curation; Formal analysis; Methodology. ALMG: Conceptualization; Writing-review and editing; Resources; Visualization; Funding acquisition. LGCP: Conceptualization; Data curation; Formal analysis; Writing-review and editing; Investigation; Methodology; Resources; Visualization; Validation; Funding acquisition; Project administration. VVV: Conceptualization; Data curation; Formal analysis; Writing-review and editing; Investigation; Methodology; Resources; Visualization; Validation; Funding acquisition; Project administration; Supervision.

Corresponding author

Correspondence to Lincoln de Oliveira Sant’Anna.

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Ethical approval

This study was approved by Research Ethics Committee of Hospital Universitário Pedro Ernesto (CEP/HUPE– CAAE: 25847614.8.0000.5259).

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de Oliveira Sant’Anna, L., dos Santos, L.S., Araújo, M.R.B. et al. Corynebacterium guaraldiae sp. nov.: a new species of Corynebacterium from human infections. Braz J Microbiol 54, 779–790 (2023). https://doi.org/10.1007/s42770-023-00938-y

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