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Exploration of non-phytopathogenic Aspergillus spp. isolates recovered from soil and compost as potential source of bioactive metabolites for potato Fusarium dry rot control

  • Soil and Agricultural Microbiology - Research Paper
  • Published:
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Abstract

During storage, infected potato tubers by Fusarium species leads to significant losses. Searching natural-based alternatives to chemical fungicides for the control of tuber dry rot pathogens is becoming essential. Nine Aspergillus spp. (A. niger, A. terreus, A. flavus, and Aspergillus sp.) isolates, recovered from soil and compost samples, were explored and evaluated for their ability to suppress Fusarium sambucinum the main causal agent of potato tuber dry rot disease in Tunisia. All conidial suspensions of Aspergillus spp. tested and their cell-free culture filtrates had significantly inhibited the in vitro pathogen growth by 18.5 to 35.9% and by 9 to 69% compared to control, respectively. A. niger CH12 cell-free filtrate was the most active against F. sambucinum at the three concentration tested (10, 15, and 20% v v−1). Chloroform and ethyl acetate extracts from four Aspergillus spp., tested at 5% v v−1, had limited F. sambucinum mycelial growth by 34–60% and 38–66%, respectively, compared to control, with A. niger CH12 ethyl extract being the most active. Tested on potato tubers inoculated with F. sambucinum, all tested Aspergillus spp. isolates, their cell-free filtrates and organic extracts had significantly decreased the external diameter of dry rot lesion compared to pathogen-inoculated and untreated control tubers. For the rot penetration, all Aspergillus spp. isolates, their organic extracts and only filtrates from A. niger CH12 and MC2 isolates had significantly limited dry rot severity compared to pathogen-inoculated and untreated control. The highest reductions in the external diameter of dry rot lesion (76.6 and 64.1%) and the average rot penetration (77.1 and 65.1%) were achieved using chloroform and ethyl acetate extracts from A. niger CH12, respectively. These results clearly demonstrated the presence of bioactive compounds in Aspergillus spp. that can be extracted and explored as an eco-friendly alternative for the control of the target pathogen.

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References

  1. Vipul G, Singh A, Vimal M, Ashwini P, Chhatpar H (2006) Bioprospecting and antifungal potential of chitinolytic microorganisms. Afr J Biotechnol 5:54–72

    Google Scholar 

  2. Khanzada MA, Tanveer M, Maitlo SA, Hajano J, Ujjan AA, Syed RN, Lodhi AM, Rajput AQ (2016) Comparative efficacy of chemical fungicides, plant extracts and bio-control agents against Fusarium solani under laboratory conditions. Pak J Phytopathol 28:133–139

    Google Scholar 

  3. Daami-Remadi M, Hibar K, Jabnoun-Khiareddine H, Ayed F, El Mahjoub M (2006) Effect of two Trichoderma species on severity of potato tuber dry rot caused by Tunisian Fusarium complex. Int J Agri Res 1:432–441

    Article  Google Scholar 

  4. 2022. www.guidephytosanitaire.tn. Accessed 10/09/2022

  5. Ferreira FV, Musumeci MA (2021) Trichoderma as biological control agent: scope and prospects to improve efficacy. World J Microbiol Biotechnol 37:90. https://doi.org/10.1007/s11274-021-03058-7

    Article  PubMed  Google Scholar 

  6. Buddhika UVA, Abeysinghe S (2021) Secondary metabolites from microbes for plant disease management. In: Singh, K.P., Jahagirdar, S., Sarma, B.K. (Eds), Emerging Trends in Plant Pathology. Springer, Singapore, 331–342. https://doi.org/10.1007/978-981-15-6275-4_15

  7. Gawai DU (2018) Role of fungi as biocontrol agents for the control of plant diseases in sustainable agriculture. In: Gehlot P, Singh J (eds) Fungi and their role in sustainable development: current perspectives. Springer, Singapore, pp 283–291

    Chapter  Google Scholar 

  8. Hiltunen LH, Tarvainen O, Kelloniemi J, Tanskanen J, Karhu J, Valkonen JPT (2021) Soil bacterial community in potato tuberosphere following repeated applications of a common scab suppressive antagonist. Appl Soil Ecol 167:e104096. https://doi.org/10.1016/j.apsoil.2021.104096

    Article  Google Scholar 

  9. Bian JY, Fang YL, Song Q, Sun ML, Yang JY, Ju YW, Li DW, Huang L (2021) The fungal endophyte Epicoccum dendrobii as a potential biocontrol agent against Colletotrichum gloeosporioides. Phytopathol 111:293–303. https://doi.org/10.1094/PHYTO-05-20-0170-R

    Article  CAS  Google Scholar 

  10. Hassine M, Aydi Ben Abdallah R, Jabnoun-Khiareddine H, Daami-Remadi M (2022) Soil-borne and compost-borne Penicillium sp and Gliocladium spp as potential microbial biocontrol agents for the suppression of anthracnose-induced decay on tomato fruits. Egyptian J Biol Pest Control 32:20. https://doi.org/10.1186/s41938-022-00519-5

    Article  Google Scholar 

  11. Bhattacharyya PN, Jha DK (2011) Optimization of cultural conditions affecting growth and improved bioactive metabolite production by a subsurface Aspergillus strain TSF 146. Int J Appl Biol Pharm Technol 2:133–143

    Google Scholar 

  12. Fu J, Zhou Y, Li HF, Ye YH, Guo JH (2011) Antifungal metabolites from Phomopsis sp. BY 254, endophytic fungus in Gossypium hirsutum. Afr J Microbiol Res 5:1231–1236

    Article  Google Scholar 

  13. Senthilkumar G, Madhanraj P, Panneerselvam A (2011) Studies on the compounds and its antifungal potentiality of fungi isolated from paddy fields soils of Jenbagaparam village, Thanjavar District, and south India. Asian J Pharm biological Res 1:19–21

    Google Scholar 

  14. Ben Mrid R, Benmrid B, Hafsa J, Boukcim H, Sobeh M, Yasri A (2021) Secondary metabolites as biostimulant and bioprotectant agents. Sci Tot Environ 777:146–204. https://doi.org/10.1016/j.scitotenv.2021.146204

    Article  CAS  Google Scholar 

  15. Daami-Remadi M, Jabnoun-Khiareddine H, Ayed F, Hibar K, Znaïdi IEA, El Mahjoub M (2006) In vitro and in vivo evaluation of individually compost fungi for potato Fusarium dry rot biocontrol. J Biol Sci 6:572–580

    Article  Google Scholar 

  16. Segaran G, Sathiavelu M (2019) Fungal endophytes: a potent biocontrol agent and a bioactive metabolites reservoir. Biocatalysis Agri Biotechnol 21:101–284. https://doi.org/10.1016/j.bcab.2019.101284

    Article  Google Scholar 

  17. Aydi Ben Abdallah R, Hassine M, Jabnoun-Khiareddine H, Haouala R, Daami-Remadi M (2014) Antifungal activity of culture filtrates and organic extracts of Aspergillus spp. against Pythium ultimum. Tun J Plant Prot 9:17–30

    Google Scholar 

  18. Mejdoub Trabelsi B, Aydi Ben Abdallah R, Ammar N, Daami Remadi M (2017) Antifungal potential of extracellular metabolites from Penicillium spp. and Aspergillus spp. naturally associated to potato against Fusarium species causing tuber dry rot. J Microb Biochem Technol 9:181–190. https://doi.org/10.4172/1948-5948.1000364

    Article  CAS  Google Scholar 

  19. Moragrega C, Carmona A, Llorente I (2021) Biocontrol of Stemphylium vesicarium and Pleospora allii on Pear by Bacillus subtilis and Trichoderma spp.: preventative and curative effects on inoculum production. Agronomy 11:1455. https://doi.org/10.3390/agronomy11081455

    Article  Google Scholar 

  20. Daami-Remadi M (2012) Potato Fusarium dry rot in Tunisia: current status and future prospects. Pest Technol 6(Special Issue 1):15–22

    Google Scholar 

  21. Mejdoub-Trabelsi B, Jabnoun-Khiareddine H, Daami-Remadi M (2015) Interactions between four Fusarium species in potato tubers and consequences for fungal development and susceptibility assessment of five potato cultivars under different storage temperature. J Plant Pathol Microbiol 6:293

    Article  Google Scholar 

  22. Atoui AK (2006) Approche de la mycotoxinogenèse chez Aspergillus ochraceus et Aspergillus carbonarius. Etudes moléculaires et physiologiques. Thèse, Institut National Polytechnique de Toulouse, Toulouse, France, p 245

    Google Scholar 

  23. Seneviratne CJ, Fong PHL, Wong SSW, Lee VHF (2015) Antifungal susceptibility and phenotypic characterization of oral isolates of a black fungus from a nasopharyngeal carcinoma patient under radiotherapy. BMC Oral Health 15:39–46. https://doi.org/10.1186/s12903-015-0023-9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Ordaz JJ, Fente CA, Va’zquez BI, Francoa CM, Cepeda A (2003) Development of a method for direct visual determination of aflatoxin production by colonies of the Aspergillus flavus group. Int J Food Microbiol 83:219–225. https://doi.org/10.1016/S0168-1605(02)00362-8

    Article  CAS  Google Scholar 

  25. Nawangsih AA, Damayanti I, Wiyono S, Kartika JG (2011) Selection and characterization of endophytic bacteria as biocontrol agents of tomato bacterial wilt disease. J Biosci 18:66–70

    Google Scholar 

  26. Barbosa MAG, Rehn KG, Menezes M, Mariano RLR (2001) Antagonism of Trichoderma species on Cladosporium herbarum and their enzymatic characterization. Braz J Microbiol 32:98–104

    Article  CAS  Google Scholar 

  27. Al-Shibli H, Dobretsov S, Al-Nabhani A, Maharachchikumbura SSN, Rethinasamy V, Al-Sadi AM (2019) Aspergillus terreus obtained from mangrove exhibits antagonistic activities against Pythium aphanidermatum-induced damping-off of cucumber. PeerJ 7:e7884. https://doi.org/10.7717/peerj.7884

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Kaewchai S, Soytong K (2010) Application of biofongicides against Rigidoporus microporus causing white root disease of rubber trees. J Agr Technol 6:349–363

    Google Scholar 

  29. Benítez T, Rincón MA, Limón MC, Codón CA (2004) Biocontrol mechanisms of Trichoderma strains. Int microbial 7:249–260

    Google Scholar 

  30. El-Katatny MH, Gudelj M, Robra KH, Elnaghy MA, Gubitz GM (2001) Characterization of a chitinase and an endo-β-1, 3- glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii. Appl Microbiol Biotechnol 56:137–143

    Article  CAS  PubMed  Google Scholar 

  31. Kusari S, Singh S, Jayabaskaran C (2014) Biotechnological potential of plant associated endophytic fungi: hope versus hyphe. Trends Biotech 32:297–303

    Article  CAS  Google Scholar 

  32. Vibha, (2010) Effect of fungal metabolites and amendments on mycelium growth of Rhizoctonia solani. J Plant Prot Res 50:93–97

    Article  Google Scholar 

  33. Sreedevi B, Charitha DM, Saigopal DVR (2011) Inoculation and screening of effective Trichoderma spp. against the root rot pathogen Macrophomina phaseolina. J Agri Technol 7:623–635

    Google Scholar 

  34. Chen LH, Cui YQ, Yang XM, Zhao DK, Shen QR (2012) An antifungal compound from Trichoderma harzianum SQR-T037 effectively controls Fusarium wilt of cucumber in continuously cropped soil. Aus Plant Pathol 41:239–245

    Article  CAS  Google Scholar 

  35. Abdel-Motaal FF, Nassar MSM, El-Zayat SA, El-Sayed MA, Ito SI (2010) Antifugal activity of endophytic fungi isolated from egyptian henbane (Hyoscyamus muticus L.). Pak J Bot 42:2883–2894

    Google Scholar 

  36. Theis T, Wedde M, Meyer V, Stahl U (2003) The antifungal protein from Aspergillus giganteus causes membrane permeabilization. Antimicrob Agents Chemother 47:588–593

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Mohammed GJ, Kadhim MJ, Hussein HM (2016) Characterization of bioactive chemical compounds from Aspergillus terreus and evaluation of antibacterial and antifungal activity. Int J Pharm Phytochem Res 8:889–905

    Google Scholar 

  38. Shemshura ON, Bekmakhanova NE, Mazunina MN, Meyer SLF, Rice CP, Masler EP (2016) Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus. FEMS Microbiol Lett 363:1–9

    Article  Google Scholar 

  39. Haggag WM, Mohamed HAA (2007) Biotechnological aspects of microorganisms used in plant biological control. American-Eurasian J Sust Agri 1:7–12

    Google Scholar 

  40. Howell CR (2003) Mechanisms employed by Trichoderma species in the biological control of plant diseases. The History and Evolution of Current Concepts. Plant Dis 87:4–10

    Article  CAS  PubMed  Google Scholar 

  41. Lin C, Yang J, Sun H, Huang X, Wang R, Zhang KO (2007) Purification and characterization of a β-1, 3 glucanase from the novel mycoparasite Periconia byssoides. Biotechnol Letter 29:617–622

    Article  CAS  Google Scholar 

  42. Haran S, Schisler H, Oppenheim A, Chet I (1996) Differential expression of Trichoderma harzianum chitinases during mycoparasitism. Phytopathol 86:980–985

    Article  CAS  Google Scholar 

  43. Kerkeni A, Daami-Remadi M, Tarchoun N, Ben Khedher M (2007) In vitro and in vivo suppression of Fusarium oxysporum f. sp. radicis-lycopersici the causal agent of Fusarium crown and root rot of tomato by some compost fungi. Int J Agr Res 2:1022–1029

    Article  Google Scholar 

  44. Cherif M, Sadfi N, Benhamou N, Boudabbous A, Boubaker A, Hajlaoui MR, Tirilly Y (2002) Ultrastructure and cytochemistry on in vitro interactions of the antagonistic bacteria Bacillus cereus X16 and B. thuringiensis 55T with Fusarium roseum var. sambucinum. J Plant Pathol 84:83–93

    Google Scholar 

  45. Sadfi N, Cherif M, Fliss I, Boudabbous A, Antoun H (2001) Evaluation of bacterial isolates from salty soils and Bacillus thuringiensis strains for the biocontrol of Fusarium dry rot of potato tubers. J Plant Pathol 83:101–118

    CAS  Google Scholar 

  46. Slininger PJ, Schisler DA, Burkhead KD, Bothast RJ (2003) Postharvest biological control of potato sprouting by Fusarium dry rot suppressive bacteria. Biological Sci Technol 13:477–497

    Article  Google Scholar 

  47. Slininger PJ, Dunlap CA, Schisler DA (2010) Polysaccharide production benefits dry storage survival of the biocontrol agent seudomonas fluorescens S11: P:12 effective against several maladies of stored potatoes. Biocontrol Sci Technol 20:227–244

    Article  Google Scholar 

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Funding

This work was funded by the Ministry of Higher Education and Scientific Research of Tunisia through the funding allocated to the research unit UR13AGR09-Integrated Horticultural Production in the Tunisian Centre East (evolved to LR21AGR03-Production and Protection for a Sustainable Horticulture (2PHD)), University of Sousse, Regional Research Centre on Horticulture and Organic Agriculture of Chott- Mariem, Tunisia.

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Authors and Affiliations

  1. LR21AGR03- Production and Protection for a Sustainable Horticulture (2PHD), Regional Research Centre On Horticulture and Organic Agriculture, University of Sousse, 4042, Chott-Mariem, Tunisia

    Rania Aydi Ben Abdallah, Hayfa Jabnoun-Khiareddine & Mejda Daami-Remadi

  2. LR14AGR01, Laboratory of Genetics and Cereal Breeding, National Agronomic Institute of Tunisia, University of Carthage, Avenue Charles Nicolle 43, 1082, Tunis, Tunisia

    Marwa Hassine

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  1. Rania Aydi Ben Abdallah
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Correspondence to Rania Aydi Ben Abdallah.

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Aydi Ben Abdallah, R., Hassine, M., Jabnoun-Khiareddine, H. et al. Exploration of non-phytopathogenic Aspergillus spp. isolates recovered from soil and compost as potential source of bioactive metabolites for potato Fusarium dry rot control. Braz J Microbiol 54, 1103–1113 (2023). https://doi.org/10.1007/s42770-023-00925-3

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