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Antimicrobial and antibiofilm activity of silver nanoparticles against Salmonella Enteritidis

  • Food Microbiology - Research Paper
  • Published:
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Abstract

Salmonella enterica serotype Enteritidis is one of the main pathogens associated with foodborne illnesses worldwide. Biofilm formation plays a significant role in the persistence of pathogens in food production environments. Owing to an increase in antimicrobial resistance, there is a growing need to identify alternative methods to control pathogenic microorganisms in poultry environments. Thus, this study aimed to synthesize silver nanoparticles (AgNPs) and evaluate their antibiofilm activity against poultry-origin Salmonella Enteritidis in comparison to a chemical disinfectant. AgNPs were synthesized, characterized, and tested for their minimum inhibitory concentration, minimum bactericidal concentration, and antibiofilm activity against S. Enteritidis strains on polyethylene surfaces. The synthesized AgNPs, dispersed in a liquid medium, were spherical in shape with a mean diameter of 6.2 nm. AgNPs exhibited concentration-dependent bactericidal action. The bacterial reduction was significantly higher with AgNPs (3.91 log10 CFU \(\bullet\) cm−2) than that with sanitizer (2.57 log10 CFU ∙ cm−2). Regarding the time of contact, the bacterial count after a contact time of 30 min was significantly lower than that after 10 min. The AgNPs exhibited antimicrobial and antibiofilm activity for the removal of biofilms produced by S. Enteritidis, demonstrating its potential as an alternative antimicrobial agent. The bactericidal mechanisms of AgNPs are complex; hence, the risk of bacterial resistance is minimal, making nanoparticles a potential alternative for microbial control in the poultry chain.

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Funding

This work was supported by the National Council for Scientific and Technological Development (CNPq) through a scholarship concession to Brunna Dias de Emery.

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Authors and Affiliations

  1. Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande Do Sul, Av. Bento Goncalves 9090, Porto Alegre, Rio Grande Do Sul, 91540-000, Brazil

    Brunna Dias de Emery, Gabriela Zottis Chitolina, Thales Quedi Furian, Karen Apellanis Borges, Hamilton Luiz de Souza Moraes, Carlos Tadeu Pippi Salle & Vladimir Pinheiro do Nascimento

  2. Laboratório de Catálise Molcular, Instituto de Química, Universidade Federal do Rio Grande Do Sul, Av. Bento Gonçalves 9500, Porto Alegre, Rio Grande Do Sul, 91501-570, Brazil

    Muhammad Irfan Qadir

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  1. Brunna Dias de Emery
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  2. Gabriela Zottis Chitolina
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  3. Muhammad Irfan Qadir
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Contributions

B. D. E., M. I. Q., T. Q. F., C. T. P. S., H. L. S. M., and V. P. N. conceived and designed the experiments. B. D. E., G. Z. C., and M. I. Q. performed experiments. B. D. E., K. A. B., and T. Q. F. analyzed the data. B. D. E., K. A. B., and T. Q. F. prepared the manuscript. All authors critically reviewed and approved the manuscript.

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Correspondence to Karen Apellanis Borges.

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Dias de Emery, B., Zottis Chitolina, G., Qadir, M.I. et al. Antimicrobial and antibiofilm activity of silver nanoparticles against Salmonella Enteritidis. Braz J Microbiol 54, 285–292 (2023). https://doi.org/10.1007/s42770-022-00868-1

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