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Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020


Different approaches are in use to improve our knowledge about the causative agent of coronavirus disease (COVID-19). Cell culture-based methods are the better way to perform viral isolation, evaluate viral infectivity, and amplify the virus. Furthermore, next-generation sequencing (NGS) have been essential to analyze a complete genome and to describe new viral species and lineages that have arisen over time. Four naso-oropharyngeal swab samples, collected from April to July of 2020, were isolated and sequenced aiming to produce viral stocks and analyze the mutational profile of the found lineage. B.1.1.33 was the lineage detected in all sequences. Although the samples belong to the same lineage, it was possible to evaluate different mutations found including some that were first described in these sequences, like the S:H655Y and T63N. The results described here can help to elicit how the pandemic started to spread and how it has been evolving in south Brazil.

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We thank the Brazilian Coordination for the Improvement of Higher-Level Personnel (CAPES), Foundation for Research Support of the State of Rio Grande do Sul (FAPERGS), and Brazilian National Council for Scientific Development (CNPq) for scholarships.


This work is an initiative of Rede Corona-ômica BR MCTI/FINEP affiliated to RedeVírus/MCTI (FINEP = 01.20.0029.000462/20, CNPq = 404096/2020–4).

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Authors and Affiliations



JSG, JDF, FRS: conceptualization. JSG, MSS, MF, MD, KS, AWH, VMAGP, FHH, VG, MNW, PRA, BLA, MD, PM, LAB, JDF, FRS: formal analysis and investigation. OAD, FRS: funding acquisition. JDF, FRS: supervision. FRS: project administration. JSG: writing—original draft. CVB, OAD, JDF, FRS: writing—review and editing.

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Correspondence to Juliana Schons Gularte.

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Project approved by the Research Ethics Committee (CEP) at Feevale University. Process number: CAAE: 33202820.7.1001.5348.

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Responsible Editor: Jônatas Abrahão

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Gularte, J.S., da Silva, M.S., Filippi, M. et al. Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020. Braz J Microbiol (2022).

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  • SARS-CoV-2
  • B.1.1.33
  • S:H655Y
  • S:T63N
  • Southern Brazil