Abstract
The aim of our study is to determine the discriminatory power of the Fourier transform infrared spectroscopy (FTIR), using multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) as molecular typing references. The study included seventeen isolates (OXA-23- and OXA-58-producing Acinetobacter baumannii) previously recovered from clinical specimens during the period May 2010–April 2011. Molecular typing was performed by PFGE and MLST. The specimens were analyzed in quadruplicate using the IR Biotyper (Bruker GmbH, Bremen, Germany). For each isolate, the average of the spectra was used for the analysis of the data. Comparing FTIR data with MLST, the results obtained by IR Biotyper are very consistent with those from MLST, since the software was able to differentiate the three ST assigned to the strains. Comparing FTIR data with PFGE, most results could be confirmed, as IR Biotyper clearly differentiated ST-80 SLV OXA-58-producing A. baumannii (pulsotype 3) from the rest of strains of OXA-58-producing A. baumannii (pulsotypes 1 and 2). All the OXA-23-producing A. baumannii isolates (pulsotype 4) grouped together by FTIR. FTIR proved to be an effective tool to investigate local epidemiology, and can achieve the same typeability and discriminatory power as genome-based methods.
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Raw data were generated at Hospital Universitario Puerta del Mar. Derived data supporting the findings of this study are available from the corresponding author on request.
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Acknowledgements
Dr. Felipe Fernández-Cuenca, Hospital Universitario Virgen Macarena, is acknowledged for his collaboration in MLST and PFGE typing.
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I.G.L., F.G.S., and M.R.I. planned the study. I.G.L. performed the FTIR analysis. I.G.L., F.G.S., and M.R.I. analyzed and evaluated the results. I.G.L. and F.G.S. wrote the manuscript. All authors revised the final version of the manuscript.
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Guerrero-Lozano, I., Galán-Sánchez, F. & Rodríguez-Iglesias, M. Fourier transform infrared spectroscopy as a new tool for surveillance in local stewardship antimicrobial program: a retrospective study in a nosocomial Acinetobacter baumannii outbreak. Braz J Microbiol 53, 1349–1353 (2022). https://doi.org/10.1007/s42770-022-00774-6
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DOI: https://doi.org/10.1007/s42770-022-00774-6