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Diversity and antimicrobial potential of the culturable rhizobacteria from medicinal plant Baccharis trimera Less D.C.

  • Environmental Microbiology - Research Paper
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Abstract

Plant microbiota is usually enriched with bacteria producers of secondary metabolites and represents a valuable source of novel species and compounds. Here, we analyzed the diversity of culturable root-associated bacteria of the medicinal native plant Baccharis trimera (Carqueja) and screened promising isolates for their antimicrobial properties. The rhizobacteria were isolated from the endosphere and rhizosphere of B. trimera from Ponta Grossa and Ortigueira localities and identified by sequencing and restriction analysis of the 16S rDNA. The most promising isolates were screened for antifungal activities and the production of siderophores and biosurfactants. B. trimera presented a diverse community of rhizobacteria, constituted of 26 families and 41 genera, with a predominance of Streptomyces and Bacillus genera, followed by Paenibacillus, Staphylococcus, Methylobacterium, Rhizobium, Tardiphaga, Paraburkholderia, Burkholderia, and Pseudomonas. The more abundant genera were represented by different species, showing a high diversity of the microbiota associated to B. trimera. Some of these isolates potentially represent novel species and deserve further examination. The communities were influenced by both the edaphic properties of the sampling locations and the plant niches. Approximately one-third of the rhizobacteria exhibited antifungal activity against Sclerotinia sclerotiorum and Colletotrichum gloeosporioides, and a high proportion of isolates produced siderophores (25%) and biosurfactants (42%). The most promising isolates were members of the Streptomyces genus. The survey of B. trimera returned a diverse community of culturable rhizobacteria and identified potential candidates for the development of plant growth-promoting and protection products, reinforcing the need for more comprehensive investigations of the microbiota of Brazilian native plants and habitats.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are grateful to Ph.D. Adriana Pereira da Silva for her assistance in the physicochemical analysis of the soil.

Funding

Partial financial support was received from the National Institute of Science and Technology, INCT-Plant-Growth Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility (CNPq 465133/2014–4, Fundação Araucária-STI 043/2019, CAPES) and the Brazilian National Council for Scientific and Technological Development (CNPq 480080/2013–5). This work was also supported by the infrastructure of the LAMM Facility from Londrina State University. Ana Camila Munis Jardim and Luana de Moura Alves received MSc scholarships from the National Council for the Improvement of Higher Education (CAPES)—Finance code 001. Jéssica Ellen de Oliveira and Giovana Oliveira Gutuzzo received a BASc scholarships from the Araucaria Foundation.

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  1. Laboratório de Genética de Microrganismos, Departamento de Biologia Geral, Universidade Estadual de Londrina, PR-445, Km 380, Campus Universitário, PO Box 6001, Londrina, Paraná, CP 86.051-970, Brazil

    Ana Camila Munis Jardim, Jéssica Ellen de Oliveira, Luana de Moura Alves, Giovana Oliveira Gutuzzo & Elisete Pains Rodrigues

  2. Laboratório de Bioquímica de Microrganismos, Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina, PR-445, Km 380, Campus Universitário, PO Box 6001, Londrina, Paraná, CP 86.051-970, Brazil

    André Luiz Martinez de Oliveira

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All authors contributed to the study conception and design or the acquisition, analysis, or interpretation of data. Material preparation, data collection, and analysis were performed by Ana Camila Munis Jardim, Jéssica Ellen de Oliveira, Luana de Moura Alves, Giovana Oliveira Gutuzzo, André Luiz Martinez de Oliveira, and Elisete Pains Rodrigues. The first draft of the manuscript was written by Ana Camila Munis Jardim and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Jardim, A.C.M., de Oliveira, J.E., Alves, L.d. et al. Diversity and antimicrobial potential of the culturable rhizobacteria from medicinal plant Baccharis trimera Less D.C.. Braz J Microbiol 53, 1409–1424 (2022). https://doi.org/10.1007/s42770-022-00759-5

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