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Arbuscular mycorrhizal fungi inoculation stimulates the production of foliar secondary metabolites in Passiflora setacea DC.

  • Environmental Microbiology - Research Paper
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Abstract

Passiflora setacea DC. growing is of interest to the herbal industries since in its leaves are produced secondary metabolites that confer antioxidant, anxiolytic, and antidepressant properties in Passiflora. Therefore, it is important to search for sustainable alternatives that aim to enhance the production of these compounds to add value to the phytomass, such as the inoculation with arbuscular mycorrhizal fungi (AMF) and the application of coconut coir dust, which has not been reported to P. setacea yet. The aim was to select the efficient combination of AMF and coconut coir dust to increase the compounds’ production and optimize the antioxidant activity in P. setacea leaves. The P. setacea seedlings that were cultivated in substrates without coconut coir dust and colonized by Gigaspora albida N.C. Schenck & G.S. Sm. produced more total saponins (1,707.43%), total tannins (469.98%), and total phenols (85.81%), in comparison to the non-mycorrhizal plants, in addition to enhancing the glomalin-related soil proteins. On the other hand, in general, the use of coir dust as a substrate has not been shown to increase the production of these bioactive compounds. It is concluded that the production of P. setacea seedlings using G. albida is an alternative to offer phytomass to the herbal medicines industry based on passion fruit.

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Acknowledgements

This research was supported by the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) — Finance Code 001. The authors would like to thank Embrapa Cerrados (Brazil) for donating Passiflora setacea seeds.

Funding

This research was supported by the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) — Finance Code 001.

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Authors and Affiliations

  1. Laboratório de Análises, Pesquisas e Estudos em Micorrizas (LAPEM/UPE) – Centro de Pesquisas do Instituto de Ciências Biológicas, Universidade de Pernambuco, Rua Arnóbio Marquês, 310, Santo Amaro – Recife, PE, 50100-130, Brazil

    Brena Coutinho Muniz, Eduarda Lins Falcão & Fábio Sérgio Barbosa da Silva

  2. Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada – ICB/UPE, Rua Arnóbio Marquês, 310, Santo Amaro – Recife, PE, 50100-130, Brazil

    Brena Coutinho Muniz, Eduarda Lins Falcão & Fábio Sérgio Barbosa da Silva

  3. Instituto de Ciências Biológicas – ICB, Universidade de Pernambuco, Rua Arnóbio Marquês, 310, Santo Amaro – Recife, PE, 50100-130, Brazil

    Fábio Sérgio Barbosa da Silva

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  1. Brena Coutinho Muniz
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  2. Eduarda Lins Falcão
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Contributions

Conceptualization: Silva, F.S.B.; data curation: Muniz, B.C., Silva, F.S.B.; formal analysis: Muniz, B.C., Silva, F.S.B.; funding acquisition: Silva, F.S.B.; investigation: Muniz, B.C., Falcão, E.L.; methodology: Muniz, B.C., Falcão, E.L., Silva, F.S.B.; project administration: Silva, F.S.B.; resources: Silva, F.S.B.; supervision: Silva, F.S.B.; visualization: Muniz, B.C., Falcão, E.L., Silva, F.S.B.; writing — original draft preparation: Muniz, B.C., Falcão, E.L., Silva, F.S.B.; writing — review and editing: Muniz, B.C., Falcão, E.L., Silva, F.S.B.

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Correspondence to Fábio Sérgio Barbosa da Silva.

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Muniz, B.C., Falcão, E.L. & da Silva, F.S.B. Arbuscular mycorrhizal fungi inoculation stimulates the production of foliar secondary metabolites in Passiflora setacea DC.. Braz J Microbiol 53, 1385–1393 (2022). https://doi.org/10.1007/s42770-022-00752-y

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