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The secondary metabolites from Beauveria bassiana PQ2 inhibit the growth and spore germination of Gibberella moniliformis LIA

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Fungal secondary metabolites with antimicrobial properties are used for biological pest control. Their production is influenced by several factors as environment, host, and culture conditions. In the present work, the secondary metabolites from fermented extracts of Beauveria bassiana PQ2 were tested as antifungal agents against Gibberella moniliformis LIA. The L18 (21 × 37) orthogonal array from Taguchi methodology was used to assess 8 parameters (pH, agitation, sucrose, yeast extract, KH2PO4, MgSO4, NH4NO3, and CaCl2) in B. bassiana PQ2 submerged fermentation. The ability of the fermented extracts to slow down the growth rate of G. moniliformis LIA was evaluated. The results from 18 trials were analyzed by Statistica 7 software by evaluating the signal-to-noise ratio (S/N) to find the lower-the-better condition. Optimal culture conditions were pH, 5; agitation, 250 rpm; sucrose, 37.5 g/L−1; yeast extract, 10 g/L−1; KH2PO4, 0.8 g/L−1; MgSO4, 1.2 g/L−1; NH4NO3, 0.1 g/L−1; and CaCl2, 0.4 g/L−1, being the agitation at the highest level the most significant factor. The optimal conditions were validated in a sparged bottle bioreactor resulting in a higher S/N value (12.48) compared to the estimate. The extract obtained has the capacity to inhibit the germination of G. moniliformis spores at 24 h. HPLC-ESI-MS2 allowed to identify the water-soluble red pigment as oosporein (m/z 304.9). The secondary metabolites from B. bassiana PQ2 are a suitable alternative to control the growth and sporulation of G. moniliformis.

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Data Availability

The dataset used in the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors want to thank to Tecnológico Nacional de México and to the Autonomous University of Nuevo León for the financial support.

Funding

The work was partially financed by the Autonomous University of Nuevo León with the project PAICyT-UANL (CT1525-21) and by Tecnológico Nacional de México with the projects No. 6691.18-P and 10394.21-P.

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Authors and Affiliations

  1. Food Research Laboratory, Facultad de Estudios Profesionales Zona Huasteca, Universidad Autónoma de San Luis Potosí, Ciudad Valles, 79060, San Luis Potosí, México

    José Guadalupe Ávila-Hernández & María Luisa Carrillo-Inungaray

  2. Departamento de Ingenierías, Tecnológico Nacional de México/I. T. de Ciudad Valles, Ciudad Valles, 79010, San Luis Potosí, México

    Pedro Aguilar-Zárate, Mariela R. Michel, Jorge Enrique Wong-Paz & Diana Beatriz Muñiz-Márquez

  3. School of Agronomy, Chemistry and Biochemistry Laboratory, Campus Ciencias Agropecuarias, Universidad Autónoma de Nuevo León, General Escobedo, 66050, Nuevo León, México

    Romeo Rojas-Molina & Guillermo Cristian G. Martínez-Ávila

  4. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, 25280, Coahuila, México

    Juan Alberto Ascacio-Valdés

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  1. José Guadalupe Ávila-Hernández
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Contributions

JGAV and MLCI performed the experimental work, data analysis, and wrote the manuscript. MRM, JEWP, DBMM, and RRM provided lab resources and reviewed the original draft. JAAV performed the chromatographic analysis. GCGMA and PAZ conceptualized the work, data analysis, and fund acquisition. All the authors approved the final manuscript.

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Correspondence to Pedro Aguilar-Zárate or Guillermo Cristian G. Martínez-Ávila.

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Ávila-Hernández, J.G., Aguilar-Zárate, P., Carrillo-Inungaray, M.L. et al. The secondary metabolites from Beauveria bassiana PQ2 inhibit the growth and spore germination of Gibberella moniliformis LIA. Braz J Microbiol 53, 143–152 (2022). https://doi.org/10.1007/s42770-021-00668-z

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