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Molecular genotyping reveals inter-regional relatedness among antimicrobial resistant Salmonella Minnesota strains isolated from poultry farm and humans, Brazil

  • Veterinary Microbiology - Short Communication
  • Published:
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Abstract

Genetic profiles of Salmonella Minnesota isolates were analyzed using pulsed-field gel electrophoresis (PFGE). In total, 13 isolates obtained from the broiler industry collected in the states of Minas Gerais (11) and São Paulo (2), as well as five recovered from cases of foodborne infections in humans in the states of Minas Gerais (2), Santa Catarina (1), and Rio Grande do Sul (2), were submitted to PFGE. These 18 S. Minnesota isolates together with other 12 of poultry origin were also subjected to antimicrobial susceptibility testing. The PFGE analysis of 18 strains of S. Minnesota generated a dendrogram that grouped the isolates with 83–90% similarity into four main clusters. Among them, cluster “A” grouped the majority of isolates (13), including two of human origin that showed 90% similarity with a broiler isolate, both recovered in Minas Gerais. The S. Minnesota isolates showed resistance to tetracycline (80%), cefoxitin (80%), ceftazidime (46.7%), nalidixic acid (23.3%), ciprofloxacin (13.3%), and streptomycin (10%). No resistance to gentamicin, chloramphenicol, meropenem, nitrofurantoin, and sulfamethoxazole-trimethoprim was found. Moreover, 23.3% of the evaluated isolates presented multi-resistance profile, all from Minas Gerais. The results highlight the importance of further studies involving S. Minnesota, which is prevalent in the Brazilian broiler flocks and could provoke foodborne infection in humans.

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Acknowledgements

We are grateful to TECSA Laboratory and to the staff of the Laboratory of Enterobacteria of the Oswaldo Cruz Foundation (FioCruz) for the strains and technical support.

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Authors and Affiliations

  1. Department of Preventive Veterinary Medicine, School of Veterinary, Federal University of Minas Gerais (UFMG), Avenida Antônio Carlos, 6667 - São Luiz, Belo Horizonte, Minas Gerais, CEP: 31270-010, Brazil

    João Paulo Fernandes Ferreira Moreira, Nelson Rodrigo da Silva Martins & Oliveiro Caetano de Freitas Neto

  2. Laboratory of Avian Pathology, Department of Pathology, Theriogenology, and One Health, São Paulo State University (FCAV-Unesp), Jaboticabal, São Paulo, Brazil

    Daniel Farias Marinho do Monte & Angelo Berchieri Junior

  3. Veterinary Science Graduate Program, School of Veterinary, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil

    Camila de Aguiar Lima

  4. Department of Animal Science, Center for Agricultural Sciences, Federal University of Paraiba (CCA/UFPB), Areia, PB, Brazil

    Celso José Bruno de Oliveira

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  1. João Paulo Fernandes Ferreira Moreira
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  2. Daniel Farias Marinho do Monte
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  3. Camila de Aguiar Lima
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  4. Celso José Bruno de Oliveira
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  6. Angelo Berchieri Junior
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  7. Oliveiro Caetano de Freitas Neto
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All authors whose names appear on the submission made substantial contributions to the conception of the work, interpretation of data, and critical review, agreeing to be accountable for all aspects of the work.

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Correspondence to Oliveiro Caetano de Freitas Neto.

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Moreira, J.P.F.F., do Monte, D.F.M., Lima, C.d. et al. Molecular genotyping reveals inter-regional relatedness among antimicrobial resistant Salmonella Minnesota strains isolated from poultry farm and humans, Brazil. Braz J Microbiol 53, 503–508 (2022). https://doi.org/10.1007/s42770-021-00666-1

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