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Enhanced antibacterial effect against Enterococcus faecalis by silver ions plus Triton X-100 with low concentrations and cytotoxicity

  • Clinical Microbiology - Research Paper
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Brazilian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Enterococcus faecalis (E. faecalis) is commonly considered to be one of chief culprits of secondary and persistent root canal infections. As antibiotic resistance has become a global issue, in order to reduce the use of antibiotics, metal ions have recently been widely used as an alternative. Silver ions (Ag+) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Triton X-100 (TX-100) and Ag+ were co-used for the first time as a clinical intracanal medication to obtain both enhanced antibacterial effect and low cytotoxicity. The synergistic antibacterial effect of TX-100 + Ag+ was tested on both planktonic and biofilm-resident E. faecalis on dentine. And the cytotoxicity was tested on MC3T3-E1 cells. Results confirmed the antibacterial activity against both planktonic and biofilm-resident E. faecalis was dramatically improved after TX-100 incorporation. TX-100 and Ag+ mixture demonstrated a similar inhibitory effect as the 2% chlorhexidine (CHX), while the cytotoxicity was much lower than 2% CHX (p < 0.05). In conclusion, TX-100 + Ag+ mixture might be developed into a new effective intracanal medication as the 2% CHX.

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All data generated or analyzed during this study are included in this published article.

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Funding

This study was financially supported by the National Natural Science Foundation of China (Grant No. 81570969&81771067) and Top Youth Talent in Medicine Programme of Hubei Province, China.

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Authors and Affiliations

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China

    Mengting Duan, Qing Sun, Wei Fan & Bing Fan

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  1. Mengting Duan
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  2. Qing Sun
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  3. Wei Fan
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  4. Bing Fan
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Contributions

Mengting Duan performed the experiment and prepared the manuscript; Qing Sun helped carry out the experiment and data analysis; Wei Fan and Bing Fan are the corresponding authors who supervised the study, provided part of the funding support, designed the experiment, and revised the manuscript.

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Correspondence to Wei Fan or Bing Fan.

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Duan, M., Sun, Q., Fan, W. et al. Enhanced antibacterial effect against Enterococcus faecalis by silver ions plus Triton X-100 with low concentrations and cytotoxicity. Braz J Microbiol 53, 161–169 (2022). https://doi.org/10.1007/s42770-021-00643-8

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  • DOI: https://doi.org/10.1007/s42770-021-00643-8

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