Abstract
Enterococcus faecalis (E. faecalis) is commonly considered to be one of chief culprits of secondary and persistent root canal infections. As antibiotic resistance has become a global issue, in order to reduce the use of antibiotics, metal ions have recently been widely used as an alternative. Silver ions (Ag+) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Triton X-100 (TX-100) and Ag+ were co-used for the first time as a clinical intracanal medication to obtain both enhanced antibacterial effect and low cytotoxicity. The synergistic antibacterial effect of TX-100 + Ag+ was tested on both planktonic and biofilm-resident E. faecalis on dentine. And the cytotoxicity was tested on MC3T3-E1 cells. Results confirmed the antibacterial activity against both planktonic and biofilm-resident E. faecalis was dramatically improved after TX-100 incorporation. TX-100 and Ag+ mixture demonstrated a similar inhibitory effect as the 2% chlorhexidine (CHX), while the cytotoxicity was much lower than 2% CHX (p < 0.05). In conclusion, TX-100 + Ag+ mixture might be developed into a new effective intracanal medication as the 2% CHX.
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This study was financially supported by the National Natural Science Foundation of China (Grant No. 81570969&81771067) and Top Youth Talent in Medicine Programme of Hubei Province, China.
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Mengting Duan performed the experiment and prepared the manuscript; Qing Sun helped carry out the experiment and data analysis; Wei Fan and Bing Fan are the corresponding authors who supervised the study, provided part of the funding support, designed the experiment, and revised the manuscript.
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Duan, M., Sun, Q., Fan, W. et al. Enhanced antibacterial effect against Enterococcus faecalis by silver ions plus Triton X-100 with low concentrations and cytotoxicity. Braz J Microbiol 53, 161–169 (2022). https://doi.org/10.1007/s42770-021-00643-8
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DOI: https://doi.org/10.1007/s42770-021-00643-8