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Genome profiling of fluoroquinolone-resistant uropathogenic Escherichia coli isolates from Brazil

  • Bacterial and Fungal Pathogenesis - Research Paper
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Abstract

Urinary tract infections (UTIs) are a major public health concern in both community and hospital settings worldwide. Uropathogenic Escherichia coli (UPEC) is the main causative agent of UTI and increasingly associated with antibiotic resistance. Herein, we report the draft genome sequence of 9 fluoroquinolone-resistant UPEC isolates from Brazil and examine selected major phenotypic features, such as antimicrobial resistance profile, phylogroup, serotype, sequence type (ST), virulence genes, and resistance marks. Besides the quinolone resistance, beta-lactams, ESBL production, aminoglycosides, and tetracycline resistance were observed. High prevalence of 20 virulence genes was detected in all isolates, such as those encoding type 1 fimbriae, acid tolerance system, and hemolysin E, particularly within E. coli B2 phylogroup, as ST131 and ST1193 strains, among other genomic analyses as genomic islands, resistance plasmids, and integron identification.

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Acknowledgements

We thank Cefar for providing the antimicrobial drug disks used in this study.

Funding

This study is funded by the CAPES, CNPq, and FAPESP Project 2014/06779–2 and 2019/03049-7.

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Authors and Affiliations

  1. Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil

    Patrick da Silva, Bruna C. Lustri & Cristiano G. Moreira

  2. Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil

    Ivana Giovannetti Castilho & Rodrigo T. Hernandes

  3. Clinical Analysis Laboratory, School of Medicine, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil

    Adriano Martison Ferreira

  4. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia

    Mark A. Schembri

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  1. Patrick da Silva
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Correspondence to Cristiano G. Moreira.

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da Silva, P., Lustri, B.C., Castilho, I.G. et al. Genome profiling of fluoroquinolone-resistant uropathogenic Escherichia coli isolates from Brazil. Braz J Microbiol 52, 1067–1075 (2021). https://doi.org/10.1007/s42770-021-00513-3

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