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Antimicrobial compounds produced by Lysinibacillus odysseyi epiphytic bacteria associated with red algae

Brazilian Journal of Microbiology volume 51, pages 1683–1690 (2020)Cite this article

Abstract

Seaweed epiphytic bacteria are highly host specific and their association is little known. The marine environment of Andaman Islands is rich in algal diversity and their association with microbial communities remains unexplored. We investigated the epiphytic bacterial communities from the intertidal red alga Gracilaria canaliculata. A total of four epiphytic bacterial isolates were cultured and screened for antimicrobial activity. Of the four isolates, one potential isolate, Gr102, exhibited strong antimicrobial activity in 0.50 μg/ml concentration against enteropathogenic Escherichia coli, Klebsiella pneumoniae, and Shigella flexneri. This potential isolate was characterized by genomic sequencing, fatty acid methyl ester analysis, and matrix-assisted laser desorption ionization-time of flight spectrometry, and the strain Gr102 was identified as Lysinibacillus odysseyi KC149512. Purified fractions obtained were analyzed by gas chromatography and mass spectrometry. Antimicrobial compounds such as furan, lupenol, diazene, and butenyl methyl ketone were identified. Based on the high level of activity, red algae epiphytic bacteria are potential sources of producing bioactive secondary metabolites.

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Acknowledgments

This work is a part of the Ph.D. thesis of the first author. The authors thank the Pondicherry University authorities for providing the facilities. Thanks to Dr. Anas Abdul Aziz, scientist, MMRF, NIO (RC), Cochin, for permitting the laboratory facilities. Also, thanks to Mr. Craig Kunitsky, MIDI, Laboratories, Newark, USA, for analyzing MALDI-TOF in this study.

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  1. Department of Ocean Studies and Marine Biology, Pondicherry University,Brookshabad Campus, Port Blair, Andaman and Nicobar Islands, 744112, India

    Perumal Karthick & Raju Mohanraju

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  1. Perumal Karthick
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  2. Raju Mohanraju
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Correspondence to Perumal Karthick.

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Karthick, P., Mohanraju, R. Antimicrobial compounds produced by Lysinibacillus odysseyi epiphytic bacteria associated with red algae. Braz J Microbiol 51, 1683–1690 (2020). https://doi.org/10.1007/s42770-020-00341-x

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  • DOI: https://doi.org/10.1007/s42770-020-00341-x

Keywords

  • Andaman
  • Antimicrobial
  • L. odysseyi
  • Gracilaria