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Canine sporotrichosis: polyphasic taxonomy and antifungal susceptibility profiles of Sporothrix species in an endemic area in Brazil

  • Veterinary Microbiology - Research Paper
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Abstract

Sporotrichosis, a mycosis caused by pathogenic species of the genus Sporothrix, affects diverse species of mammals. Until 2007, Sporothrix schenckii was considered the unique etiologic agent of sporotrichosis. Canine sporotrichosis is a poorly reported disease, and the majority of cases are from Rio de Janeiro, Brazil. There are scarce studies on the characterization of canine isolates of Sporothrix schenckii complex, as well as few antifungal susceptibility data available. The aim of this study was to characterize the clinical isolates of Sporothrix from dogs from Brazil at species level and evaluate their antifungal susceptibility profile. Polyphasic taxonomy was used to characterization at species level (morphological, phenotypical characteristics, and molecular identification). Antifungal susceptibility profiles (amphotericin B, itraconazole, ketoconazole, posaconazole, and terbinafine) were determined using the Clinical and Laboratory Standards Institute broth microdilution method (M38-A2). According to phenotypic identification and molecular analysis, 46 isolates included in this study were identified as S. brasiliensis and one as S. schenckii. Amphotericin B presented the highest minimum inhibitory concentration values, and the other drugs showed effective in vitro antifungal activity. This is the first report of S. schenckii in dogs from Brazil, since S. brasiliensis is the only species that has been described in canine isolates from Rio de Janeiro to date. Nevertheless, no differences were observed in the antifungal susceptibility profiles between the S. brasiliensis and S. schenckii isolates, and it is important to continuously study new canine clinical isolates from Rio de Janeiro, Brazil.

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Acknowledgments

The authors are grateful for access to the Oswaldo Cruz Foundation’s sequencing platform (PDTIS/Fiocruz).

Funding

This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001 and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro—FAPERJ (E-26/200.839/2017). SAP was supported in part by Jovem Cientista do Nosso Estado 2016 (JCNE)—FAPERJ (grant no.: E-26/203.303/2016) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grant no.: 309657/2016-4). RMZ-O was supported in part by CNPq (grant no.: 304976/2013-0) and FAPERJ (grant no.: E-26/103.157/2011). MMO was supported in part by FAPERJ (grant no.: INST E-26/010.001784/2016, JCNE E-26/203.301/2017).

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Authors and Affiliations

  1. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365 – Manguinhos, Rio de Janeiro, 21040-900, Brazil

    Jéssica Sepulveda Boechat, Sandro Antonio Pereira, Ana Caroline de Sá Machado, Paula Gonçalves Viana & Isabella Dib Ferreira Gremião

  2. Mycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil

    Rodrigo Almeida-Paes & Rosely Maria Zancopé-Oliveira

  3. Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil

    Manoel Marques Evangelista de Oliveira

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  1. Jéssica Sepulveda Boechat
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  2. Sandro Antonio Pereira
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  3. Ana Caroline de Sá Machado
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  4. Paula Gonçalves Viana
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Correspondence to Jéssica Sepulveda Boechat.

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The study was approved by the Ethics Committee on Animal Use of the Oswaldo Cruz Foundation (CEUA/Fiocruz; number: LW-17/17).

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Boechat, J.S., Pereira, S.A., de Sá Machado, A.C. et al. Canine sporotrichosis: polyphasic taxonomy and antifungal susceptibility profiles of Sporothrix species in an endemic area in Brazil. Braz J Microbiol 52, 135–143 (2021). https://doi.org/10.1007/s42770-020-00328-8

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