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Human serum proteins bind to Sporothrix schenckii conidia with differential effects on phagocytosis

  • Bacterial and Fungal Pathogenesis - Short Communication
  • Published:
Brazilian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Serum is an important source of proteins that interact with pathogens. Once bound to the cell surface, serum proteins can stimulate the innate immune system. The phagocytosis of Sporothrix schenckii conidia by human macrophages is activated through human serum opsonisation. In this study, we have attempted to characterise human blood serum proteins that bind to the cell wall of S. schenckii conidia. We systematically observed the same four proteins independent of the plasma donor: albumin, serum amyloid protein (SAP), α-1 antitrypsin (AAT), and transferrin were identified with the help of tandem mass spectrometry. Phagocytosis depended on the concentration of the SAP or α-1 antitrypsin that was used to opsonise the conidia; however, transferrin or albumin did not have any effect on conidia internalisation. The presence of mannose did not affect macrophage phagocytosis of the conidia opsonised with SAP or α-1 antitrypsin, which suggests that these proteins are not recognised by the mannose receptor.

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Acknowledgements

We acknowledege the skilful and invaluable assistance of Gabriela Perera Slazar, Unidad de Investigación, Marco Gudiño Zayas and Angelica Serrano Ahumada, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, UNAM. This work ws supported by DGAPA IN214013, UNAM.

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Authors and Affiliations

  1. Departamento de Investigación en Microbiología, Instituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Ciudad de México, 14502, Mexico

    Silvia Guzman Beltrán

  2. Unidad de Investigación en Medicina Experimental, Micología Básica, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México “Dr. Eduardo Liceaga”, Ciudad de México, Mexico

    Jazmín Sanchez Morales & Haydee Torres Guerrero

  3. Unidad de Investigación en Medicina Experimental, Patología Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México “Dr. Eduardo Liceaga”, Ciudad de México, Mexico

    Augusto González Canto

  4. Unidad Periferica de la Facultad de Medicina, Unidad de Investigación en Medicina Traslacional. Inmunobioquímica Molecular y Cardiopatías, Ciudad de México, 14080, Mexico

    Alma Escalona Montaño

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  1. Silvia Guzman Beltrán
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  2. Jazmín Sanchez Morales
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Correspondence to Haydee Torres Guerrero.

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Guzman Beltrán, S., Sanchez Morales, J., González Canto, A. et al. Human serum proteins bind to Sporothrix schenckii conidia with differential effects on phagocytosis. Braz J Microbiol 52, 33–39 (2021). https://doi.org/10.1007/s42770-020-00276-3

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  • DOI: https://doi.org/10.1007/s42770-020-00276-3

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