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Teschovirus and other swine and human enteric viruses in Brazilian watersheds impacted by swine husbandry

  • Environmental Microbiology - Research Paper
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Abstract

Several emerging viral agents related to gastroenteritis are distributed in human and animal populations and may contaminate the environment due to anthropic activities. The objective of this study was to analyze the seasonal contamination by enteric virus and coliforms in water from streams in the Vale do Taquari, draining a large number of pig farms. Microbiological contamination was evidenced by the detection of total and thermotolerant coliforms, reaching their peak in December. Hepatitis E virus (HEV), Enterovirus-G (EV-G) genome, and Sapelovirus-A (SV-A) genome were not detected. On the other hand, Rotavirus (RV) was detected in 3% (1/32) of the samples, whereas Teschovirus-A (PTV) was detected in 6% (2/32). This is the first detection of PTV in environmental samples in Brazil, pointing that the virus is being shedded from swine herds to watersheds. Human mastadenovirus (HAdV) was the most frequent detected viral agent in 9.3% (3/32) with values of 2.54 × 105, 7.13 × 104, and 3.09 × 105 genome copies/liter (gc/L). The circulation of coliforms and viral pathogens is noticeable due to anthropic activities and to the management of animal waste from the pig farming. In this way, enteric viruses can assist in monitoring the quality of watersheds and in tracking sources of contamination.

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Acknowledgements

This work was supported by Feevale University, the Coordination for the Improvement of Higher Level Personnel (CAPES), Rio Grande do Sul Secretariat for Economic Development, Science and Technology, and the Brazilian National Research Council (CNPq). FRS is a CNPq fellow.

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Authors and Affiliations

  1. Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, Rio Grande do Sul, Brazil

    F. G. Souza, J. S. Gularte, M. Demoliner, A. F. Lima, J. C. Siebert, Caroline Rigotto, Andreia Henzel, A. K. A. Eisen & F. R. Spilki

  2. Laboratório de Microbiologia Molecular, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, Novo Hamburgo, Rio Grande do Sul, 93352-000, Brazil

    F. G. Souza

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  1. F. G. Souza
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  2. J. S. Gularte
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  3. M. Demoliner
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  4. A. F. Lima
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  5. J. C. Siebert
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  6. Caroline Rigotto
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  8. A. K. A. Eisen
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  9. F. R. Spilki
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Correspondence to F. G. Souza.

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Souza, F.G., Gularte, J.S., Demoliner, M. et al. Teschovirus and other swine and human enteric viruses in Brazilian watersheds impacted by swine husbandry. Braz J Microbiol 51, 711–717 (2020). https://doi.org/10.1007/s42770-019-00197-w

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  • DOI: https://doi.org/10.1007/s42770-019-00197-w

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