Abstract
The aim of this study is to report the occurrence of Lymphocystivirus in Brazilian ornamental fish. From 25 ornamental fish species submitted for molecular diagnosis, only one sample (Pomacanthus imperator) was positive, and its viral nucleotide sequence obtained clustered with sequences of genotype VII. To our knowledge, this is the first report on the genetic characterization of Lymphocystivirus in Brazil.
References
Livengood EJ, Chapman FA (2007) The ornamental fish trade: an introduction with perspectives for responsible aquarium fish ownership. In: FA124 Department of Fisheries and Aquatic Sciences. Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, p 1–8. https://edis.ifas.ufl.edu/fa124
Ploeg A (2007) The volume of the ornamental fish trade. In: Fossa S, GMO B, Chuan LL, Ploeg A (eds) International Transport Of Live Fish In The Ornamental Aquatic Industry. Ornamental Fish International Educational Publication 2. OFI, Maarssen, pp 48–64
Rimmer AE, Becker JA, Tweedie A, Lintermans M, Landos M, Stephens F, Whittington RJ (2015) Detection of dwarf gourami iridovirus (Infectious spleen and kidney necrosis virus) in populations of ornamental fish prior to and after importation into Australia, with the first evidence of infection in domestically farmed platy (Xiphophorus maculatus). Prev Vet Med 2122:181–194
Chinchar VG, Waltzek TB, Subramaniam K (2017) Ranavirus and other members of the family Iridoviridae: their place in the virosphere. Virology 17:30184–30188
Cano I, Valverde EJ, Lopez-Jimena B, Alonso MC, Garcia-Rosado E, Sarasquete C, Borrego JJ, Castro D (2010) A new genotype of Lymphocystivirus isolated from cultured gilthead seabream, Sparus aurata L., and Senegalese sole, Solea senegalensis (Kaup). Fish Dis 33:695–700
Alonso MC, Cano I, Garcia-Rosado E, Castro D, Lamas J, Barja JL, Borrego JJ (2005) Isolation of lymphocystis disease virus from sole, Solea senegalensis Kaup, and blackspot sea bream, Pagellus bogaraveo (Brünnich). J Fish Dis 28:221–228
Ciulli S, Pinheiro ACAS, Volpe E, Moscato M, Jung TS, Galeotti M, Stellino S, Farneti R, Prosperi S (2015) Development and application of a real-time PCR assay for the detection and quantitation of lymphocystis disease virus. J Virol Methods 213:164–173
Palmer LJ, Hogan NS, VanDenHeuvel MR (2012) Phylogenetic analysis and molecular methods for the detection of lymphocystis disease virus from yellow perch. Perca flavescens (Mitchell). Fish Dis 35:661–667
Chinchar VG, Hick P, Ince IA, Jancovich JK, Marschang R, Qin Q, Subramaniam K, Waltzek TB, Whittington R, Williams T, Zhang Q, Report Consortium ICTV (2017) ICTV virus taxonomy profile: Iridoviridae. J Gen Virol 98:890–891
Wolf K (1988) Fish viruses and fish viral diseases. Cornell University Press, Ithaca
Colorni A, Diamant A (1995) Splenic and cardiac lymphocystis in the red drum, Scaenops ocellatus (L.). Fish Dis 18:467–471
Cano I, Valverde EJ, Garcia-Rosado E, Alonso MC, Lopez-Jimena B, Ortiz-Delgado JB, Borrego JJ, Sarasquete C, Castro D (2013) Transmission of lymphocystis disease virus to cultured gilthead seabream, Sparus aurata L., larvae. Fish Dis 36:569–576
Anders K. 1989 Lymphocystis disease of fishes. In: Viruses of lower vertebrates (ed. by W. Ahne & D. Kurstak), Springer-Verlag, Berlin; . p. 141–160
Hossain M, Song JY, Kitamura SI, Jung SJ, Oh MJ (2008) Phylogenetic analysis of lymphocystis disease virus from tropical ornamental fish species based on a major capsid protein gene. J Fish Dis 31:473–479
Iwamoto R, Hasegawa O, LaPatra S, Yoshimizu M (2008) Isolation and characterization of the Japanese flounder (Paralichthys olivaceus) lymphocystis disease virus. J Aquat Anim Health 14:114–123
Cardoso PHM, Balian SC (2016) Lymphocystis virus in ornamental fish imported in Brazil. J Contin Educ Anim Sci CRMV-SP 14:6–11
Tidona CA, Schnitzler P, Kehm R, Darai G (1998) Is the major capsid protein of iridoviruses a suitable target for the study of viral evolution? Virus Genes 16:59–66
Xu L, Feng J, Huang Y (2014) Identification of lymphocystis disease virus from paradise fish Macropodus opercularis (LCDV-PF). Arch Virol 159:2445–2449
Kitamura SI, Jung SJ, Oh MJ (2006) Differentiation of Lymphocystis disease virus genotype by multiplex PCR. J Microbiol 44:248–253
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Kumar S, Stecher G, Tamura K (2016) MEGA 7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods 14:587–589
Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772
Campanella JJ, Bitincka L, Smalley J (2003) MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics 4:1–4
Cano I, Ferro P, Alonso MC, Bergmann SM, Römer-Oberdörfer A, Garcia-Rosado E, Castro D, Borrego JJ (2007) Development of molecular techniques for detection of lymphocystis disease virus in different marine fish species. J Appl Microbiol 102:32–40
Kvitt H, Heinisch G, Diamant A (2008) Detection and phylogeny of Lymphocystivirus in sea bream Sparus aurata based on the DNA polymerase gene and major capsid protein sequences. Aquaculture 275:58–63
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This research was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, No. 2014/04327-7 and No. 2012/08846-3).
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de Lucca Maganha, S.R., Cardoso, P.H.M., de Carvalho Balian, S. et al. Detection and molecular characterization of Lymphocystivirus in Brazilian ornamental fish. Braz J Microbiol 51, 531–535 (2020). https://doi.org/10.1007/s42770-019-00196-x
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DOI: https://doi.org/10.1007/s42770-019-00196-x