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Detection and molecular characterization of chicken infectious anaemia virus in young chicks in Punjab region of north-western India

  • Veterinary Microbiology - Research Paper
  • Published:
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Abstract

Chicken infectious anaemia (CIA) is an important viral disease of chicken causing significant immunosuppression and severe anaemia worldwide. Occurrence of severe disease and mortality is noticed in young chicks (2–3 weeks). Vertical mode of transmission increases chance of infection and persistence of virus among the infected flocks. The current study was conducted in Punjab state for confirmation and genetic characterization of CAV among chicken flocks of various poultry farms. DNA was extracted from the tissue samples and subjected to polymerase chain reaction (PCR) of VP1 gene and whole genome. PCR products were further sequenced for confirmation of chicken infectious anaemia virus (CIAV) genome in the clinical samples. PCR amplification of DNA from the tissue samples yielded expected product size of 1350 bases of VP1 gene and 2.3 kb of whole genome. Out of 16 commercial poultry farms, 11 were confirmed with presence of CIAV, and out of 65 birds, 39 were found positive (60%) for CIAV genes. Among the various organs, the presence of viral gene was detected at highest level in thymus when compared with other organs. It is concluded that chicken infectious anaemia virus detected from Punjab state is closely related to other Indian isolates and neighbouring countries which necessitates need of more intensive studies with a greater number of samples for implementing effective control measures.

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Acknowledgements

Authors are thankful to the Director of Research, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana for providing necessary facilities.

Funding

The Department of Science and Technology (DST) granted the project and financial assistance was received from DST in the form of WOS-A fellowship to the first author.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, College of Veterinary Science, GADVASU, Ludhiana, Punjab, 141004, India

    S. M. Sreekala & K. Gurpreet

  2. Department of Dairy Microbiology, College of Dairy Science & Technology, GADVASU, Ludhiana, Punjab, 141004, India

    P. N. Dwivedi

Authors
  1. S. M. Sreekala
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  2. K. Gurpreet
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  3. P. N. Dwivedi
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Correspondence to S. M. Sreekala.

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Sreekala, S.M., Gurpreet, K. & Dwivedi, P.N. Detection and molecular characterization of chicken infectious anaemia virus in young chicks in Punjab region of north-western India. Braz J Microbiol 51, 805–813 (2020). https://doi.org/10.1007/s42770-019-00160-9

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