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High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

  • Bacterial Fungal and Virus Molecular Biology - Research Paper
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Abstract

Salmonella Dublin is a strongly adapted serovar that causes enteritis and/or systemic disease with high rates of mortality in cattle and occasionally infects humans. Despite the importance of this serovar, there is a lack of studies in Brazil. The aim of this study was to characterize the genetic diversity of 112 S. Dublin strains isolated from humans and animals in Brazil by CRISPR and CRISPR-MVLST and the relatedness among strains by MLST. In addition, the frequency of some important virulence genes was verified. The strains studied belonged to nine different sequence types, being all of them single- or double-locus variants of the ST10. CRISPR discriminated the strains into 69 subtypes with a similarity ≥ 84.4% and CRISPR-MVLST into 72 subtypes with a similarity ≥ 84.7%. The virulence genes ratB, lpfA, mgtC, avrA, sopB, sopE2, sifA, sseA, ssrA, csgA, fliC, and sinH were found in all the strains studied, while spvB, spvC, sodCl, rpoS, sipA, sipD, invA, and hilA were detected in ≥ 93.7% of the strains. In conclusion, the high similarity among the strains reinforces the clonal nature of the strains of this serovar that may have descended from a common ancestor that little differed over 33 years in Brazil. CRISPR and CRISPR-MVLST showed to be good alternatives to type S. Dublin strains. MLST suggested that S. Dublin strains from Brazil were phylogenetically related to strains from other parts of the globe. Moreover, the high frequency of virulence genes among the strains studied reinforces the capacity of S. Dublin to cause invasive diseases.

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Acknowledgments

The authors thank São Paulo Research Foundation - FAPESP for financial support and Dr. Marc Allard from FDA/CFSAN for providing whole-genome sequencing of the strains studied.

Funding

This study was financed by São Paulo Research Foundation (FAPESP) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. During the course of the work, F.P. Vilela was supported by a scholarship from FAPESP (Proc. 2017/05756-7 and 2019/06947-6) and F. Campioni was supported by a postdoctoral fellowship from FAPESP (Proc. 2013/25191-3). Part of this work was also financed by a FAPESP grant (Proc. 2016/24716-3) under the supervision of J.P. Falcão that also received a productive fellowship (CNPq 303475/2015-3 and CNPq 304399/2018-3) from National Council for Scientific and Technological Development (CNPq).

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Authors and Affiliations

  1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto – USP, Av. do Café, s/n, Ribeirão Preto, SP, 14040-903, Brazil

    Felipe Pinheiro Vilela, Juliana Pfrimer Falcão & Fábio Campioni

  2. Fundação Oswaldo Cruz – FIOCRUZ, Pavilhão Rocha Lima, Av. Brasil, 4365, Rio de Janeiro, RJ, 21040-900, Brazil

    Dália dos Prazeres Rodrigues & Renata Garcia Costa

  3. Centro de Bacteriologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, São Paulo, SP, 01255-000, Brazil

    Monique Ribeiro Tiba Casas

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  1. Felipe Pinheiro Vilela
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  2. Dália dos Prazeres Rodrigues
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  3. Renata Garcia Costa
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  4. Monique Ribeiro Tiba Casas
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  5. Juliana Pfrimer Falcão
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Correspondence to Juliana Pfrimer Falcão or Fábio Campioni.

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Vilela, F.P., dos Prazeres Rodrigues, D., Costa, R.G. et al. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil. Braz J Microbiol 51, 497–509 (2020). https://doi.org/10.1007/s42770-019-00156-5

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