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Rapid detection of the main carbapenemases in Brazil directly from spiked blood culture using the RESIST-3 O.K.N. immunoassay

  • Clinical Microbiology - Research Paper
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Abstract

The emergence of carbapenem-resistant Enterobacterales (CRE) is a matter of public health concern. Carbapenemases are the main mechanism of resistance among CRE, and its rapid detection is essential. The detection of carbapenemases usually requires culture-based methods and molecular assays, which may be costly and need long turnaround times. Recently, an easy and rapid immunochromatographic assay for carbapenemases (OXA-48, KPC, and NDM) detection based in lateral flow immunoassay with specific monoclonal antibodies on a nitrocellulose membrane has been developed. We aimed to evaluate the RESIST-3 O.K.N. in colonies from pure culture as well as in spiked blood cultures with Enterobacterales. All carbapenemase producers (CP) presenting the OXA-48-like, KPC, and NDM enzymes presented positive results in both pure colonies and spiked blood cultures. None of the carbapenemase non-producers (CNP) presented positive results in the tests. A total of 97% CP isolates presented positive results in pure colonies in less than 5 min. For CP directly from blood culture, the mean time to positivity for OXA-48-like and KPC was 1 min, whereas it was 25 min for NDM. Our results indicate that this immunoassay can be used to detect carbapenemases directly from blood culture bottles in a routine diagnostic laboratory, which would reduce the turnaround time of CP detection.

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Acknowledgments

RESIST-3 O.K.N. assays were supplied free of charge for evaluation by Serion Brasil (Curitiba, Brazil).

Funding

This work was supported by INPRA (Instituto Nacional de Pesquisa em Resistência Antimicrobiana), Brazil (INCT/CNPq: 465718/2014-0). P.L.W. and A.S.M. were supported by a grant from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). T.V.D. was supported by a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. LABRESIS (Laboratório de Pesquisa em Resistência Bacteriana), Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil

    Priscila Lamb Wink, Amanda Silva Martins, Everton Inamine, Tanise Vendruscolo Dalmolin & Afonso Luís Barth

  2. Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Priscila Lamb Wink, Tanise Vendruscolo Dalmolin & Afonso Luís Barth

  3. Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Amanda Silva Martins, Everton Inamine & Afonso Luís Barth

  4. Santa Casa de Misericórdia, Porto Alegre, RS, Brazil

    Everton Inamine

Authors
  1. Priscila Lamb Wink
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  2. Amanda Silva Martins
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  3. Everton Inamine
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  4. Tanise Vendruscolo Dalmolin
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  5. Afonso Luís Barth
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Correspondence to Afonso Luís Barth.

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Wink, P.L., Martins, A.S., Inamine, E. et al. Rapid detection of the main carbapenemases in Brazil directly from spiked blood culture using the RESIST-3 O.K.N. immunoassay. Braz J Microbiol 50, 657–662 (2019). https://doi.org/10.1007/s42770-019-00109-y

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  • DOI: https://doi.org/10.1007/s42770-019-00109-y

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