Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper respiratory tract of asymptomatic horses from Southern Brazil

  • Alais Maria Dall Agnol
  • Edsel Alves Beuttemmuller
  • Daniela Pilz
  • Raquel Arruda Leme
  • Viviane Saporiti
  • Selwyn Arlington Headley
  • Alice Fernandes Alfieri
  • Amauri Alcindo AlfieriEmail author
Veterinary Microbiology - Short Communication


Equid gammaherpesvirus 2 (EHV-2) and 5 (EHV-5) are members of the Herpesviridae family and have been reported in horse populations worldwide. This study aimed to evaluate the presence of herpesvirus DNA in the upper respiratory tract of horses. Twenty-six nasal swabs were collected from asymptomatic adult horses of two different horse farms (A, n = 18; B, n = 8), both located in Southern Brazil. The EHV-1, EHV-2, EHV-4, and EHV-5 DNA analyses were performed using nested PCR assays targeting the glycoprotein B gene. Four (15.3%) and 12 (46.1%) of the 26 nasal swab samples were positive for the EHV-2 and EHV-5, respectively. Four (15.3%) horses were detected with both viruses simultaneously. DNA of EHV-2 and EHV-5 in both single and mixed infections was identified in horses from both herds. All swab samples were negative for EHV-1 and EHV-4. This study reports the first detection of EHV-2 and EHV-5 in the upper respiratory tracts of horses in Brazil. The high detection rate of EHV-2 and EHV-5 in asymptomatic adult horses demonstrates that these gammaherpesviruses are circulating in Brazil.


Equine Herpesviruses EHV-2 EHV-5 Molecular detection Brazil 



The authors thank the following Brazilian Institutes for financial support: National Council of Technological and Scientific Development (CNPq), the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES), Financing of Studies and Projects (FINEP), and the Araucaria Foundation (FAP/PR). Alfieri, AA; Alfieri, AF; Headley, SA; and Dall Agnol, AM are recipients of CNPq fellowships. Leme, RA is a recipient of CAPES fellowship.


This study was funded by CNPq and CAPES.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  • Alais Maria Dall Agnol
    • 1
    • 2
  • Edsel Alves Beuttemmuller
    • 1
    • 3
  • Daniela Pilz
    • 1
  • Raquel Arruda Leme
    • 1
    • 3
  • Viviane Saporiti
    • 1
  • Selwyn Arlington Headley
    • 4
    • 5
  • Alice Fernandes Alfieri
    • 1
    • 3
  • Amauri Alcindo Alfieri
    • 1
    • 3
    Email author
  1. 1.Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus UniversitárioUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Center for Agroveterinary SciencesUniversidade do Estado de Santa CatarinaLagesBrazil
  3. 3.Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus UniversitárioUniversidade Estadual de LondrinaLondrinaBrazil
  4. 4.Laboratory of Animal Pathology, Department of Veterinary Preventive MedicineUniversidade Estadual de LondrinaLondrinaBrazil
  5. 5.Multi-User Animal Health Laboratory, Tissue Processing Unit, Department of Veterinary Preventive MedicineUniversidade Estadual de LondrinaLondrinaBrazil

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