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Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

Brazilian Journal of Microbiology volume 50, pages 633–648 (2019)Cite this article

Abstract

Recalcitrant characteristics and insolubility in water make the disposal of synthetic polymers a great environmental problem to be faced by modern society. Strategies towards the recycling of post-consumer polymers, like poly (ethylene terephthalate, PET) degradation/depolymerization have been studied but still need improvement. To contribute with this purpose, 100 fungal strains from hydrocarbon-associated environments were screened for lipase and esterase activities by plate assays and high-throughput screening (HTS), using short- and long-chain fluorogenic probes. Nine isolates were selected for their outstanding hydrolytic activity, comprising the genera Microsphaeropsis, Mucor, Trichoderma, Westerdykella, and Pycnidiophora. Two strains of Microsphaeropsis arundinis were able to convert 2–3% of PET nanoparticle into terephthalic acid, and when cultured with two kinds of commercial PET bottle fragments, they also promoted weight loss, surface and chemical changes, increased lipase and esterase activities, and led to PET depolymerization with release of terephthalic acid at concentrations above 20.0 ppm and other oligomers over 0.6 ppm. The results corroborate that hydrocarbon-associated areas are important source of microorganisms for application in environmental technologies, and the sources investigated revealed important strains with potential for PET depolymerization.

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Acknowledgments

The authors thank PETROBRAS for their authorization to publish this study, and to MSc. Tulio de Lucca Capelini for technical support. We thank the Center for Environmental Studies (CEA) for providing site and infrastructure to carry out part of this work.

Funding

The authors received financial support from PETROBRAS (2012/00327-7). One of the authors (L.M.P.) received a support grant from the Coordination for the Improvement of Higher Education Personnel (CAPES).

Author information

Authors and Affiliations

  1. Environmental Studies Center, UNESP, São Paulo State University, 24-A Av., 1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil

    Lusiane Malafatti-Picca & Derlene Attili-Angelis

  2. Institute of Chemistry, State University of Campinas, PO Box 6154, Campinas, SP, 13084-971, Brazil

    Michel Ricardo de Barros Chaves & Anita Jocelyne Marsaioli

  3. Biotechnology Department, R&D Center, PETROBRAS, Av. Horácio Macedo, 950, Ilha do Fundão, Rio de Janeiro, RJ, 21941-915, Brazil

    Aline Machado de Castro & Érika Valoni

  4. Division of Microbial Resources, CPQBA - State University of Campinas, Alexandre Cazellato Str., 999, Paulínia, SP, 13148-218, Brazil

    Valéria Maia de Oliveira & Derlene Attili-Angelis

  5. Department of Biochemistry and Microbiology, UNESP, São Paulo State University, 24-A Av., 1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil

    Dejanira de Franceschi de Angelis & Derlene Attili-Angelis

Authors
  1. Lusiane Malafatti-Picca
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  2. Michel Ricardo de Barros Chaves
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  3. Aline Machado de Castro
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  4. Érika Valoni
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  5. Valéria Maia de Oliveira
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  6. Anita Jocelyne Marsaioli
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  7. Dejanira de Franceschi de Angelis
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  8. Derlene Attili-Angelis
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Corresponding author

Correspondence to Lusiane Malafatti-Picca.

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Malafatti-Picca, L., de Barros Chaves, M.R., de Castro, A.M. et al. Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization. Braz J Microbiol 50, 633–648 (2019). https://doi.org/10.1007/s42770-019-00093-3

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  • DOI: https://doi.org/10.1007/s42770-019-00093-3

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