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Components of rhizospheric bacterial communities of barley and their potential for plant growth promotion and biocontrol of Fusarium wilt of watermelon

Brazilian Journal of Microbiology volume 50, pages 749–757 (2019)Cite this article

Abstract

This work aimed to characterize antagonistic bacteria from the field-grown barley rhizosphere, and evaluate their potential for growth promotion and biocontrol of Fusarium wilt on watermelon caused by Fusarium oxysporum f. sp. Niveum (FON). Seven bacteria were isolated and screened for plant growth promoting and antagonistic traits. Based on the results of phenotypic characterization and 16S rRNA gene sequencing, the isolates were identified to be related to Bacillus methylotrophicus (DMK-1), Bacillus amyloliquefaciens subsp. plantarum (DMK-7-2), Bacillus cereus (DMK-12), Pseudomonas brassicacearum subsp. brassicacearum (DMK-2), Pseudomonas veronii (DMK-3), Paenibacillus polymyxa (DMK-8), and Ensifer adhaerens (DMK-17). All the isolates were positive for the production of indole-3-acetic acid (IAA) and ammonia (NH3), while negative for the production of hydrogen cyanide (HCN). Six bacteria strains (except DMK-17) were able to phosphate solubilization. All the bacteria strains, except DMK-8, were able to produce iron siderophore complexes, and possessed the proteolytic activity. Greenhouse experiment indicated six strains can decrease diseased percentage caused by FON. All the isolates enhanced plant biomass, six strains increased root volume, six strains increased root system activity in greenhouse test. Inoculation of mixtures of seven plant growth promoting rhizobacteria could be more effective in plant growth promotion and biocontrol of Fusarium wilt in watermelon.

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Acknowledgments

The authors are very grateful to Siqin Chao, Yuting Han, Yingxuan Wang, and Xiaoyue Zhang for their valuable assistance in laboratory and greenhouse experiment.

Funding

This research was supported by the Fundamental Research Funds for the Central Universities of China (Grant No. 2013PY092).

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Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China

    Wenjie Yang

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  1. Wenjie Yang
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Correspondence to Wenjie Yang.

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Yang, W. Components of rhizospheric bacterial communities of barley and their potential for plant growth promotion and biocontrol of Fusarium wilt of watermelon. Braz J Microbiol 50, 749–757 (2019). https://doi.org/10.1007/s42770-019-00089-z

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  • DOI: https://doi.org/10.1007/s42770-019-00089-z

Keywords

  • Barley
  • Biocontrol
  • Fusarium wilt
  • Growth promotion
  • Rhizosphere bacteria