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Evaluation of microbial communities in peels of Brazilian tropical fruits by amplicon sequence analysis

  • Environmental Microbiology - Research Paper
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A Correction to this article was published on 30 November 2019

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Abstract

Elucidation of the distinctive microbial taxonomic profiles of tropical fruit peels is the indispensable component of investigations aimed at the detection of microorganisms responsible for the post-harvest loss. The objective of the present work was to dissect the bacterial and fungal community of five tropical fruit peels (banana, guava, mango, papaya, and passion fruit) in wild (non-cultivated) and conventionally produced samples from Brazil. To that end, 16S rRNA–encoding gene and ITS rDNA amplicon analysis of the five tropical fruit peels were performed to discriminate the bacterial and fungal communities, respectively. The result showed that bacterial communities of the five types of fruit peels were by far more diversified than that of fungal communities, independent of the type of production system involved. Among the investigated fruits, non-cultivated papaya peels hosted the most diversified bacterial community while the least bacterial community diversity was found in the conventionally produced papaya fruit peels. The gene amplicon analysis clearly discriminated the bacterial community into their respective classes, while fungal communities were better classified in their phyla, yet with clearer component discrimination of fungal community based on the type of cultivation system practiced. Conventionally produced banana and non-cultivated passion fruit peels were characteristically dominated by fungal and bacterial groups, respectively. Overall, in conventionally produced fruit peels, bacterial community was mainly composed of Proteobacteria, Actinobacteria, and Bacilli. The result provided a broad microbial diversity profile that could be used as an important input for seeking alternative fruit spoilage control and post-harvest treatments.

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Change history

  • 30 November 2019

    The original version of this article unfortunately contained two mistakes in the “Materials and methods” section, subsection “DNA extraction and PCR” of the article. The correct information is given below.

  • 30 November 2019

    The original version of this article unfortunately contained two mistakes in the ���Materials and methods��� section, subsection ���DNA extraction and PCR��� of the article. The correct information is given below.

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Acknowledgments

This project was supported by the Alexander von Humboldt-Foundation—AvH (Germany) and National Council for Scientific and Technological Development—CNPq (Brazil). Thanks to the Cell Biology Department, Universidade de Brasilia, for allowing us to use their facilities during the DNA extraction.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan

    André Freire Cruz

  2. Applied Biology Department, Adama Science and Technology University, Adama, Oromia, Ethiopia

    Geleta Dugassa Barka

  3. Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, Brazil

    Luiz Eduardo Bassay Blum

  4. Division of Information Science, Nara Institute of Science and Technology, Nara, Japan

    Tetsushi Tanaka, Naoaki Ono & Shigehiko Kanaya

  5. Department of Crop Protection, Geisenheim University, Geisenheim, Germany

    Annette Reineke

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Correspondence to Geleta Dugassa Barka.

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Cruz, A.F., Barka, G.D., Blum, L.E.B. et al. Evaluation of microbial communities in peels of Brazilian tropical fruits by amplicon sequence analysis. Braz J Microbiol 50, 739–748 (2019). https://doi.org/10.1007/s42770-019-00088-0

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