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Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb. Among carbon sources examined, the addition of sucrose maximizes the mycelia production, while fructose could maximize the EPS yield. Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly. FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs. The molecular weight of EPSs was also affected by carbon source, being generally lower compared with that with glucose. However, all EPSs molecule existed as nearly globular shape form in aqueous solution. The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay. Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.

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Funding

This work was supported by the National Science Foundation of China (Grant No U1604176 and 31571778).

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Authors and Affiliations

  1. Technical Center, China Tobacco Guangxi Industrial Co. Ltd, Nanning, 530001, People’s Republic of China

    Yan Wu, Dongye Huang & Zhizhong Hu

  2. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, Henan, People’s Republic of China

    Xuewei Jia, Jianqiang Zheng & Chunping Xu

Authors
  1. Yan Wu
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  2. Xuewei Jia
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  3. Dongye Huang
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  4. Jianqiang Zheng
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  5. Zhizhong Hu
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  6. Chunping Xu
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Correspondence to Chunping Xu.

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Wu, Y., Jia, X., Huang, D. et al. Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source. Braz J Microbiol 50, 625–632 (2019). https://doi.org/10.1007/s42770-019-00071-9

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  • DOI: https://doi.org/10.1007/s42770-019-00071-9

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